Assessment of Genetic Diversity in Durum and Bread Wheat Genotypes Based on Drought Tolerance and SSR Markers

Hassan, Mahdi

doi:10.9787/pbb.2021.9.2.89

Cited by 12 publications

(9 citation statements)

References 58 publications

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“…The results obtained using these SSRs are prospective markers that may be utilized to aid in selection for drought tolerance through molecular plant breeding. Furthermore, the findings are consistent with the effectiveness of SSR markers related to diversity in drought tolerance in wheat reported by Honore et al [62], Ateş Sönmezo glu and Terzi [19], Slim et al [23], Belete et al [7], and El-Rawy and Hassan [11]. In the present study, the A and D genomes contains the highest mean numbers of alleles per locus compared to the B genome.…”

Section: Discussionsupporting

confidence: 92%

“…It is commonly recognized that phenotypic and molecular methodologies have been used to investigate the variety and differentiation of wheat genetic resources, either separately or in combination [7][8][9][10][11]. There are several phenotypic tools for measuring genetic diversity; among them, crop ideotypes, phenology, and yield attributes are used extensively because of their reliability in measuring genetic variation within a gene pool [4,7,12].…”

Section: Introductionmentioning

confidence: 99%

“…A higher rate of polymorphism, along with the multi-allelic nature of SSR markers, aids in establishing relationships among genotypes, even with fewer markers [20]. Some recent studies using SSR markers that successfully analyzed wheat genetic diversity showed that genotypes were significantly diverged and should be considered in drought-tolerance breeding programs [7,11,19,21,22].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Genetic Diversity of Bread Wheat Genotypes for Drought Tolerance Using Canopy Reflectance-Based Phenotyping and SSR Marker-Based Genotyping

et al. 2022

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This study investigated the genetic diversity of bread-wheat genotypes using canopy reflectance-based vegetation indices (VIs) and simple sequence repeat (SSR) marker-based genotyping for drought tolerance. A total of 56 wheat genotypes were assessed using phenotypic traits (combination of VIs and yield traits) and 30 SSR markers. The data of the phenotypic traits were averaged over two growing seasons under irrigated and drought-stressed conditions. The hierarchical clustering of the wheat genotypes unveiled three drought-tolerant groups. Cluster 1 genotypes showed minimal phenotypic alterations, conferring superior drought tolerance and yield stability than clusters 2 and 3. The polymorphism information content values for the SSR markers ranged from 0.434 to 0.932, averaging 0.83. A total of 458 alleles (18.32 alleles per locus) were detected, with the most polymorphic markers, wmc177 and wms292, having the most alleles (24). A comparative study of SSR diversity among phenotypic clusters indicated that genotypes under cluster 1 had higher genetic diversity (0.879) and unique alleles (47%), suggesting their potential in future breeding programs. The unweighted neighbor-joining tree grouped the wheat genotypes into five major clusters. Wheat genotypes from all phenotypic clusters were distributed throughout all SSR-based clusters, indicating that genetically heterogeneous genotypes were allocated to different drought-tolerant groups. However, SSR-based clusters and model-based populations showed significant co-linearity (86.7%). The findings of the present study suggest that combining reflectance-based indirect phenotyping with SSR-based genotyping might be an effective technique for assessing genetic diversity to improve the drought tolerance of bread-wheat genotypes.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of Genetic Diversity of Bread Wheat Genotypes for Drought Tolerance Using Canopy Reflectance-Based Phenotyping and SSR Marker-Based Genotyping

et al. 2022

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“…The MT and S categories included five genotypes each, while the T category included three genotypes. HC analysis has been used for ranking the drought-tolerant wheat genotypes by many scholars [6,28,29,67,68] without validating their categories. So, we here verified categories through LD analysis, which showed that prior and posterior categories were completely identical in the thirteen genotypes used (Table 4).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, SSR markers are a suitable choice for genetic diversity studies of wheat genotypes. Some recent studies have highlighted using SSR markers, which have succeeded in wheat genetic diversity analysis and shown that genotypes have diverged to a large extent-an essential consideration in breeding programs for drought tolerance [21,25,[28][29][30][31]. The discovery of QTLs (quantitative trait loci) has made a revolution in the selection process for genes conferring quantitative traits (such as drought tolerance) in MAS, which are mostly located in the A and B genomes on chromosomes 2B, 3A, 4A, 4B, 7A, and 7B [7,8,28,32].…”

Section: Introductionmentioning

confidence: 99%

Combining Genetic and Phenotypic Analyses for Detecting Bread Wheat Genotypes of Drought Tolerance through Multivariate Analysis Techniques

Sallam,

Ghazy,

Al-Doss

et al. 2024

Life

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Successfully promoting drought tolerance in wheat genotypes will require several procedures, such as field experimentations, measuring relevant traits, using analysis tools of high precision and efficiency, and taking a complementary approach that combines analyses of phenotyping and genotyping at once. The aim of this study is to assess the genetic diversity of 60 genotypes using SSR (simple sequence repeat) markers collected from several regions of the world and select 13 of them as more genetically diverse to be re-evaluated under field conditions to study drought stress by estimating 30 agro-physio-biochemical traits. Genetic parameters and multivariate analysis were used to compare genotype traits and identify which traits are increasingly efficient at detecting wheat genotypes of drought tolerance. Hierarchical cluster (HC) analysis of SSR markers divided the genotypes into five main categories of drought tolerance: four high tolerant (HT), eight tolerant (T), nine moderate tolerant (MT), six sensitive (S), and 33 high sensitive (HS). Six traits exhibit a combination of high heritability (>60%) and genetic gain (>20%). Analyses of principal components and stepwise multiple linear regression together identified nine traits (grain yield, flag leaf area, stomatal conductance, plant height, relative turgidity, glycine betaine, polyphenol oxidase, chlorophyll content, and grain-filling duration) as a screening tool that effectively detects the variation among the 13 genotypes used. HC analysis of the nine traits divided genotypes into three main categories: T, MT, and S, representing three, five, and five genotypes, respectively, and were completely identical in linear discriminant analysis. But in the case of SSR markers, they were classified into three main categories: T, MT, and S, representing five, three, and five genotypes, respectively, which are both significantly correlated as per the Mantel test. The SSR markers were associated with nine traits, which are considered an assistance tool in the selection process for drought tolerance. So, this study is useful and has successfully detected several agro-physio-biochemical traits, associated SSR markers, and some drought-tolerant genotypes, coupled with our knowledge of the phenotypic and genotypic basis of wheat genotypes.

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Microsatellites as Potential Molecular Markers for Genetic Diversity Analysis in Plants

Sagar

Kapoor

Mahajan

2023

Molecular Marker Techniques

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Assessment of Genetic Diversity in Durum and Bread Wheat Genotypes Based on Drought Tolerance and SSR Markers

Cited by 12 publications

References 58 publications

Assessment of Genetic Diversity of Bread Wheat Genotypes for Drought Tolerance Using Canopy Reflectance-Based Phenotyping and SSR Marker-Based Genotyping

Assessment of Genetic Diversity of Bread Wheat Genotypes for Drought Tolerance Using Canopy Reflectance-Based Phenotyping and SSR Marker-Based Genotyping

Combining Genetic and Phenotypic Analyses for Detecting Bread Wheat Genotypes of Drought Tolerance through Multivariate Analysis Techniques

Microsatellites as Potential Molecular Markers for Genetic Diversity Analysis in Plants

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