Assessment of genetic diversity in Tamarind (Tamarindus indica L. ) using random amplified polymorphic DNA markers

Abstract

Determination of genetic variation is important to the plant breeders for development of high yielding variety. The aim of the current study was to investigate the genetic diversity of nine tamarind cultivars, out of nine four flowering cultivars using random amplified polymorphic DNA (RAPD) markers. Ten Random amplified polymorphic DNA (RAPD) primers were used to assess the genetic diversity in four flowering cultivars and five non-flowering of tamarind trees. The average genetic similarity level among the four flowering cultivars and five non-flowering accessions grouped into six clusters groups at 0.76%. RAPD profiles of all the tamarind were compared and a total of 58 scorable bands were produced with seven primers ranging from one for OPG-13 to twelve for OPA-R15. Genotypes which were morphological closely related were found to be unrelated at the molecular level. A sizeable amount of intrapopulation diversity recorded in the present study which can be utilized in hybridization programmes to efficiently introgress the desirable trait of interest.

Estructura genética espacial y diversidad genética de una población natural de Ramorinoa girolae en la provincia de San Juan (Argentina): Un análisis exploratorio

“…Some examples performed in monotypic species with low restricted distribution revealed that genetic polymorphism is variable. Some examples of relatively high values of this parameter are described in other monotypic species of Fabaceae such as Tamarindus indicus (Pi=76.0%, AFLP marker) (Kumar et al 2015) and Plathymenia reticulata (70.8%, RAPD marker) (Lacerda et al 2001). However, another highly threatened species shows low values of genetic polymorphism.…”

“…To date, genetic studies in arboreal species have only been performed for a limited number of species (Lacerda et al 2001;Juárez-Muñoz et al 2002;Casiva et al 2002;Juchum et al 2007; Kumar et al 2015;Martínez Araneda et al 2011). One of the main causes of the scarce of studies is the absence of molecular tools and genetic markers applicable to a wide range of species.…”

Genetic Diversity Assessment of Selected Annona muricata L. Germplasm in Sri Lanka

“…Advantage of species with rich genetic diversity like Annona muricata is that it can easily adapt and conform to a wide range of environmental conditions in comparison to none genetically diverse species as those of the domesticated crops [33]. The determination of genetic variability is important for breeders when developing varieties with better characteristics and productivity [40]. The results generated from this study can be combined with a detailed morphological characterization of soursop and this information can be used in future breeding programs to develop elite soursop cultivars and varieties in Sri Lanka.…”

“…According to the dendrogram, fifty soursop genotypes were separated into four major clusters at 80% genetic similarity level (Fig. 3) Cluster three included fourteen genotypes collected from ARS, Girandurukotte (27,28,29,30,31,32,33,34,35,36,37,38,39,40) grouped according to the geographical origin of the genotypes. The cluster provided evidence for the close proximity and similar morphology (leaf type, fruit size, pulp colour, brix value) of the genotypes within the same geographical location sharing a common origin.…”

Determination of Genetic Diversity of Tamarind (Tamarindus indica) Accessions in Eastern Region of Kenya Using Inter Simple Sequence Repeat (ISSR) Markers

“…The wide genetic variation is also aided by the large geographical distribution, adaptation, and cross-pollination [5;6]. Trees with wide variation within the population are preferred in selecting the best trees in relation to crop improvement [6]. Very little is known about tamarind's genetic improvement, and farmers choose cultivars based on observable desirable traits, especially the taste and color of the pulp [5].…”