Assessment of Genetic Diversity in Iraqi Camel Breeds using Cytochrome b

Ayied, Asaad Y.; Al-Badran, Adnan Issa; Al-Zaalan, Ayoob Radhi

doi:10.17582/journal.aavs/2018/6.7.273.277

Cited by 1 publication

(1 citation statement)

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“…These SNPs are novel to the studied Iraqi cattle. The reason behind that is that Iraq considers as an origin of sheep (Ayied & Zaqeer, 2019), camel (Ayied et al, 2018) and cattle (Faraj et al, 2019;Owaid et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Single Nucleotide Polymorphisms in the Promoter of CYP19 Gene in Cattle Bred in Iraq

Faraj

Ayied

Al-Rishdy

2020

Basrah J. Agric. Sci.

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The present study was undertaken to characterize the genetic diversity of the aromatase cytochrome P450 (CYP19) gene in 34 cows (15 local, 14 Holstein, and 5 Crosses) in Iraq. The objectives of the present study are to detect SNPs (mutations) in promoter p1.1 of the CYP19 gene in cattle bred in Iraq using sequencing techniques. We identified five single-nucleotide polymorphisms (SNP) loci of the CYP19 gene that were detected, namely G933T, G994C, A1044G, A1062T, and C1468A. The results showed the presence of 3, 4, and 2 polymorphic sites leading to the construction of 4, 5, and 3 different haplotypes for Holstein, local, and crosses respectively. Haplotype diversity were 0.791, 0.752, and 0.700 respectively. While nucleotide diversity was 0.0017, 0.0022, and 0.0013 respectively. Besides, we carried out a phylogenetic analysis of these sequences to address the evolutionary relationship between the animal species. These fragments were assigned in the GenBank database under the accession numbers: LC490756, LC490757, LC491437, LC491438, LC491439, LC491588, and LC491589.

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Section: Discussionmentioning

confidence: 99%