Assessment of genetic diversity in<i>Triticum urartu</i>Thumanjan ex Gandilyan accessions using start codon targeted polymorphism (SCoT) and CAAT-box derived polymorphism (CBDP) markers

Gholamian, Fataneh; Etminan, Alireza; Changizi, Mahdi; Khaghani, Shahab; Gomarian, Masoud

doi:10.1080/13102818.2019.1691466

Cited by 20 publications

(11 citation statements)

References 36 publications

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“…In the same context, the average PIC value for SCoT markers (0.71) was higher than ISSR (0.69), suggesting a good capacity of the SCoT system to present the polymorphic level in the investigated genotypes. Similar results were revealed by [ 51 ]. Furthermore, the higher number of alleles per locus and number of effective alleles, the more polymorphic was the population and reflected the size of the population variation.…”

Section: Discussionsupporting

confidence: 92%

Detection of genetic divergence among some wheat (Triticum aestivum L.) genotypes using molecular and biochemical indicators under salinity stress

Gowayed

El-Moneim

2021

PLoS ONE

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Wheat has remarkable importance among cereals in Egypt. Salt stress affects plant growth, development, and crop productivity. Therefore, salinity tolerance is an essential trait that must be incorporated in crops. This research aimed to investigate molecular and biochemical indicators and defence responses in seedlings of 14 Egyptian wheat genotypes to distinguish the most contrasting salt-responsive genotypes. Analysis of ISSR and SCoT markers revealed high polymorphism and reproducible fingerprinting profiles for evaluating genetic variability within the studied genotypes. The HB-10 and SCoT 1 primers had the highest values for all the studied parameters. All the tested primers generated a set of 66 polymorphic bands among tolerant and sensitive genotypes. The transcript profiles of eight TaWRKY genes showed significant induction under the salinity treatments. Moreover, the expression of TaWRKY6 for genotypes Sids 14 and Sakha 93 sharply increased and recorded the highest expression, while the expression of TaWRKY20 for Misr 1 recorded the lowest expression. Under salt stress, the total sugar, proline, and phenolic contents increased significantly, while the chlorophyll content decreased significantly. Additionally, five peroxidase and polyphenol oxidase isoforms were observed in treated leaves and clustered into five different patterns. Some isoforms increased significantly as salinity levels increased. This increase was clearer in salt-tolerant than in salt-sensitive genotypes. Eighteen protein bands appeared, most of which were not affected by salinity compared with the control, and specific bands were rare. Generally, the Sids 14, Sakha 93, Sohag 4, and Gemmeiza 12 genotypes are considered salt tolerant in comparison to the other genotypes.

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Section: Discussionsupporting

confidence: 92%

Detection of genetic divergence among some wheat (Triticum aestivum L.) genotypes using molecular and biochemical indicators under salinity stress

Gowayed

El-Moneim

2021

PLoS ONE

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“…The authors indicated that Rp and MI could be the most important indices in determining marker efficiency, while the ISSR had a higher resolution than SCoT markers, as this was consistent with our findings. Also, the results of the present study confirmed the usefulness of these markers for identifying the genetic diversity of wheat and of its wild relative genotypes [41][42][43].…”

Section: Discussionsupporting

confidence: 82%

Assessment of genetic diversity among Iranian Aegilops triuncialis accessions using ISSR, SCoT, and CBDP markers

Khodaee

Azizinezhad

Etminan

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Crop wild relatives (CWRs) are commonly used as a suitable genetic reservoir for plant breeding. They can be used for enhancing the tolerance of plant varieties to biotic and abiotic stresses. Studying the genetic diversity of related wheat species in Iran could be useful to improve different traits of bread wheat, since the country is one of the major centers of genetic diversity and distribution of Aegilops species. Therefore, the aim of the present study was to determine the relationship among 48 Aegilops triuncialis accessions using three DNA marker systems, including start codon targeted (SCoT), CAAT box-derived polymorphism (CBDP), and inter-simple sequence repeat (ISSR) markers. Results A total of 359 amplified DNA fragments were generated using 13 CBDP, 14 SCoT, and 16 ISSR primers that produced 96, 147, and 152 bands, respectively. The discriminating power of the three markers was assessed using polymorphism information content (PIC), marker index (MI), and resolving power (Rp). The mean values of PIC for ISSR, SCoT, and CBDP markers were 0.3, 0.26, and 0.34, respectively, indicating the efficiency of the three markers in detecting polymorphism among the studied accessions. ISSR markers had the highest values of MI, Rp, and polymorphism percentage as compared to SCoT and CBDP markers. Based on the Shannon index and heterozygosity values, genetic diversity in the Alborz population was more than in other populations. The accessions were classified into six, five, and five groups based on ISSR, SCoT, and CBDP using the UPGMA method. According to the results of cluster and PCoA analyses, the variation patterns corresponded with the geographical distribution of the Ae. triuncialis accessions. Conclusions The three markers provided a comprehensive pattern of the genetic diversity among the Iranian Ae. triuncialis accessions. This information could allow for a future insight into wheat breeding programs.

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“…According to Etminan et al and Khodaee et al [25,62], Rp and MI were the most relevant indices for measuring marker efficiency, although the ISSR had a higher resolution than SCoT markers, contradicting our findings. Additionally, the current study's findings verified the utility of these markers for detecting wheat and its wild cousin genotypes' genetic diversity [63,64].…”

Section: Issr Resultssupporting

confidence: 72%

SCoT, ISSR, and SDS-PAGE Investigation of Genetic Diversity in Several Egyptian Wheat Genotypes under Normal and Drought Conditions

Shaban

Arab

Basuoni

et al. 2022

International Journal of Agronomy

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Using agronomic parameters, ISSR (inter simple sequence repeat), and SCoT (start codon targeted) markers, ten potential wheat genotypes were examined for genetic diversity under normal and drought conditions. Significant agronomic features have been identified, as well as a low drought susceptibility index. Using seven SCoT and seven ISSR primers, a total of 112 amplified DNA fragments were synthesized, resulting in 61 and 51 bands, respectively. For SCoT and ISSRs, the percentage of polymorphism was 93.4% and 78.4%, respectively. Two markers, ISSR and SCoT, were found to be effective in detecting polymorphism among the examined genotypes, with mean PIC values of 0.61 and 0.62, respectively. In terms of marker index (MI), resolving power (Rp), and polymorphism percentage, SCoT markers exhibited the most significant values. The examination of seed storage proteins revealed 21 subunits with a mass ranging from 22 to 110 kDa. A cluster analysis of the data and morphological features contributed to identifying different molecular and biochemical bands that could be linked to genotype 4’s drought-resistance capabilities.

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Assessment of genetic diversity inTriticum urartuThumanjan ex Gandilyan accessions using start codon targeted polymorphism (SCoT) and CAAT-box derived polymorphism (CBDP) markers

Abstract: Gomarian (2019) Assessment of genetic diversity in Triticumurartu Thumanjan ex Gandilyan accessions using start codon targeted polymorphism (SCoT) and CAAT-box derived polymorphism (CBDP) markers,

Cited by 20 publications

References 36 publications

Detection of genetic divergence among some wheat (Triticum aestivum L.) genotypes using molecular and biochemical indicators under salinity stress

Detection of genetic divergence among some wheat (Triticum aestivum L.) genotypes using molecular and biochemical indicators under salinity stress

Assessment of genetic diversity among Iranian Aegilops triuncialis accessions using ISSR, SCoT, and CBDP markers

SCoT, ISSR, and SDS-PAGE Investigation of Genetic Diversity in Several Egyptian Wheat Genotypes under Normal and Drought Conditions

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