Assessment of genetic diversity in Clitoria ternatea populations from different parts of India by RAPD and ISSR markers

Bishoyi, Ashok Kumar; Pillai, Vipul V.; Geetha, K.; Maiti, Satyabrata

doi:10.1007/s10722-014-0145-y

Cited by 24 publications

(15 citation statements)

References 21 publications

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“…Moreover, the resolution of the selected ISSR primer ampli cation was found to be good quality as revealed by gel images indicating the usefulness of the markers for this investigation. The obtained results were strongly supported by the proven hypothesis of earlier reports, i.e., ISSR markers are veri ed as more relevant for assessing the genetic diversity where genome information of the said species is unknown (Bishoyi et al 2014;Bishoyi et al 2016a;Gupta et al 2017). The present study also revealed that occurrence CT/TC repeats are more frequent in A. paniculata genome in comparison to other possible repeats (out of a total of 15 primers, 8 primers are CT/ TC repeats) and these repeats contribute more than 60% to the total number of amplicons analyzed.…”

Section: Issr-pcr Analysissupporting

confidence: 85%

“…In this situation, it is essential to catalog the medicinal plant resources and maintain their germplasm through various strategies. Evaluation of genetic diversity using molecular markers aids in realistic conservation & crop improvement strategies and helps in deciding which germplasm to conserve on a priority basis (Morikawa and Leggett 1990;Nybom and Bartish 2000;Bishoyi et al 2014). A better understanding of genetic diversity and its distribution in the natural population is essential for plant conservation and its e cient utilization towards human welfare (Morikawa and Leggett 1990;Bishoyi et al 2016a;Bishoyi et al 2018).…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity Analysis Revealed the Hot Spot of Acmella Paniculata Existing in Natural Populations of Gujarat

Patel

Purohit

Sapra

et al. 2022

Preprint

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Genetic diversity analysis contributes to the conservation, protection and utilization of genetic resources toward efficient management of germplasm. In this investigation, Inter Simple Sequence Repeats (ISSR) markers were used to analyze the genetic diversity of 32 genotypes of Acmella paniculata collected from 12 different states of Gujarat, India. There were 113 loci amplified by 15 different ISSR markers with an average of 7.53 loci per primer. From the total 113 amplicons; 81 were polymorphic, 43 were monomorphic and 9 were found to be unique showing an average of 60.18% polymorphism. The primer (CT)8T produced a high value (0.65) of polymorphic information content (PIC); whereas, primer (TC)8RA produced a low value (0.21). The resolving power (RP) value of the primers ranged from 5.25 in primer (TC)8RA to 23.19 in primer (AC)8YT with an average of 12.17. The Jaccard’s similarity coefficients value ranged from 0.48 to 0.94. The highest percentage of similarity (94%) was found between accessions AP6 and AP16 which were collected from Panchmahal and Narmada respectively, as they are geographically closely located. Whereas, accessions AP2 from Banaskantha and AP7 from Panchmahal were most distantly related with the similarity value of 48%, as expected to their collection locations. The study revealed that there is no correlation between geographical distance and genetic diversity among the populations studied. The resultant data generated from ISSR molecular marker-based genetic diversity analysis of A. paniculata in Gujarat can provide a reference for the conservation and efficient management of the important Indian genetic resources.

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Section: Issr-pcr Analysissupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Analysis Revealed the Hot Spot of Acmella Paniculata Existing in Natural Populations of Gujarat

Patel

Purohit

Sapra

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

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“…Previous research also supports the capability of ISSRs to reveal polymorphisms compared to other random primers ( Zietkiewicz et al, 1994 ). The number of bands produced per primer varied significantly due to the number of annealing sites within the genome and primer structure ( Bishoyi et al, 2014 ). Fruit trees showing a high percentage of polymorphism are expected to be rich sources of genes for fulfilling the breeding objectives.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity analysis and population structure in apricot (Prunus armeniaca L.) grown under north-western himalayas using ISSR markers

Sheikh

Sharma

Shah

et al. 2021

Saudi Journal of Biological Sciences

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Investigation of genetic variability and population relationship of 50 accessions of the apricot ( Prunus armeniaca L.) was carried out using ISSR markers. The results revealed that the number of alleles per locus varied from 4 to 8 with a mean value of 6.75, and the mean effective number of alleles (Ne) per locus was 1.54. Similarly, the polymorphic information content (PIC) values ranged from 0.464 to 0.424, with a mean value of 0.424. The mean heterozygosity, marker index, resolving power, and effective multiplex ratio (EMR) ranged from 0.001 to 0.002, 0.01–0.06, 1.76–3.84, and 1–4.12. The dendrogram clustered genotypes into two main clades based on their origins. The population structure revealed two sub-populations with some admixtures. The average expected heterozygosity and population differentiation within two sub-populations was 0.1428 and 0.216, respectively. The results outcome reveals that the four ISSR markers comprehensively separated the indigenous germplasm from the exotic germplasm. The genetic divergence within indigenous genotypes and exotic genotypes could allow for future insights into apricot breeding programs.

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“…But in a similar study of using RFLP primers Capo-chichi et al, (2003) reported that UPGMA dendrogram generated two major clusters based on existing phenological difference with maturity. The clustering of Mucuna germplasm in two major cluster according geographical locations reported in velvet bean using RAPD primers (Padmesh et al, 2006) and also Bishoyi et al, (2014) clustering pattern was reported that all the accession of Clitoria ternatea grouped according to geographical origin rather than their morphological variation of flower colour.…”

Section: Genetic Similarity and Cluster Analysismentioning

confidence: 91%

Genetic diversity analysis in underutilized medicinal climber Mucuna pruriens (L.) DC. germplasm revealed by inter simple sequence repeats markers

Chinapolaiah

Bindu

Khadke

et al. 2018

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Velvet bean is an important medicinal legume, its seeds are prominent source of L-Dopa. The present investigation on genetic diversity assessment of 58 germplasm of velvet bean by using 11 ISSR markers. Out of 63 amplified products 59 were showed polymorphism and 4 were monomorphic with an average of 5.7 bands amplified per primer. According to band statistics and efficiency parameters showed the primers UBC 827, UBC 834 and UBC 836 were more efficient. The highest genetic similarity values (0.90) were observed between IIHR MP 102 and IIHR MP 74-3. In dendrogram germplasm grouped into two major clusters at 63 per cent similarity. Among the germplasm, IIHR Selection 4, IIHR Selection 10, IIHR MP 9, IC 33243 and IIHR MP 7 were found to be distinctly divergent, can be used in the further breeding programme.

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Assessment of genetic diversity in Clitoria ternatea populations from different parts of India by RAPD and ISSR markers

Cited by 24 publications

References 21 publications

Genetic Diversity Analysis Revealed the Hot Spot of Acmella Paniculata Existing in Natural Populations of Gujarat

Genetic Diversity Analysis Revealed the Hot Spot of Acmella Paniculata Existing in Natural Populations of Gujarat

Genetic diversity analysis and population structure in apricot (Prunus armeniaca L.) grown under north-western himalayas using ISSR markers

Genetic diversity analysis in underutilized medicinal climber Mucuna pruriens (L.) DC. germplasm revealed by inter simple sequence repeats markers

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