Assessment of genetic diversity in Dalbergia sissoo clones through RAPD profiling

Bakshi, Meena; Sharma, A. K.

doi:10.1007/s11676-011-0183-7

Cited by 8 publications

(6 citation statements)

References 6 publications

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“…The average number of polymorphic bands is 13.6 per locus, a similar value (13.8) obtained by Zheng et al (2017) on Chinese bermudagrass (Cynodon dactylon) and this a little higher than the mean number of polymorphic loci reported for 152 studies of various plant species using SRAP (Robarts and Wolfe 2014), The moderate level of polymorphic loci (56%) obtained from this study suggesting that SRAP marker is considered reliable to detect polymorphism both at individual and population level. The values were generally lower than those obtained in other previous studies using more random markers such as RAPD, ISSR and SSR (Rout et al 2003;Bakshi and Sharma 2011;Cheng et al 2016;Zheng et al 2017). (Yulita and Rahmat 2019) considered SRAP and the other mentioned markers-e.g.…”

Section: Genetic Diversity Of Dalbergia Latifoliacontrasting

confidence: 66%

Unraveling genetic variations of Dalbergia latifolia (Fabaceae) from Yogyakarta and Lombok Island, Indonesia

et al. 2020

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Abstract. Yulita KS, Atikah TD, Wardani W, Susila. 2020. Unraveling genetic variations of Dalbergia latifolia (Fabaceae) from Yogyakarta and Lombok Island, Indonesia. Biodiversitas 21: 833-841. Dalbergia latifolia Roxb. (Fabaceae) or sonokeling is a timber species extracted for its beautiful heartwood used mainly for musical instruments. D. latifolia was listed in Appendix 2 of CITES since 1997, and the demand for its timber is increasing. The species is considered vulnerable (VU A1cd ver 2.3) to extinction according to the IUCN Red List, while cultivations were mainly done from a root cutting. To anticipate the further loss of population size while maintaining sustainable use of the species, it is essential to evaluate the genetic diversity since the genetic diversity level could serve as an indicator of plant adaptability to its environment. This present study was the first to report the genetic diversity of five populations of D. latifolia from Yogyakarta and Lombok Island, West Nusa Tenggara, Indonesia. Ten combinations of Sequence Random Amplified Polymorphism (SRAP) primers were used to amplify 48 samples of D. latifolia from five populations in Yogyakarta and Lombok Island. The 137 SRAP loci were used for a cluster, and population genetic analysis, with mean NPL, was of 13.6/locus, PLP of 56.18%, H of 0.16, and PIC for all population was of 0.28. The lowest gene diversity (H = 0.11) was observed in population WL 1 of Lombok Island, while the highest (H = 0.20) was recorded for population KP of Yogyakarta. The Yogyakarta and Lombok populations were genetically structured at a medium level (Gst = 0.27), with low genetic distance (0.0310-0.1281), and a moderate level of variation observed among individual trees (ca. 0.5 coefficient similarity). The results of this present study were expected to provide necessary information on further research of D. latifolia from Indonesia to support the sustainable use of D. latifolia in Indonesia and the preservation of this species.

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Section: Genetic Diversity Of Dalbergia Latifoliacontrasting

confidence: 66%

Unraveling genetic variations of Dalbergia latifolia (Fabaceae) from Yogyakarta and Lombok Island, Indonesia

et al. 2020

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“…The results of genetic diversity indices were comparable to Dalbergia sissoo Roxb. (PIC 0.30) [51], but much lower than these reported for other Dalbergia species, such as Dalbergia monticola Bosser & R. Rabev. (He 0.83) [52], Dalbergia nigra (Vell.)…”

Section: Discussionmentioning

confidence: 92%

Genetic Diversity and Population Structure Analysis of Dalbergia Odorifera Germplasm and Development of a Core Collection Using Microsatellite Markers

Liu

Zhang

Liu

et al. 2019

Genes

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Dalbergia odorifera T. Chen (Fabaceae) is a woody tree species indigenous to Hainan Island in China. Due to its high medicinal and commercial value, this tree species has been planted over 3500 ha2 in southern China. There is an urgent need for improvement of the D. odorifera germplasm, however, limited information on germplasm collection, conservation, and assessment of genetic resources is available. Therefore, we have built a database of 251 individuals collected across the whole of southern China, which included 42 wild trees and 210 cultivated trees, with the following objectives. (1) Evaluate genetic diversity and population structure of the database using 19 microsatellite markers and (2) develop a core collection for improvement and breeding programs. Totally, the 19 microsatellite markers harbored 77 alleles across the database with the polymorphic information content (PIC) ranging from 0.03 to 0.66. Medium genetic diversity level was inferred by Nei’s gene diversity (0.38), Shannon’s information index (0.65), and observed (0.33) and expected heterozygosity (0.38). Structure analysis showed that four was the optimum cluster size using the model-based Bayesian procedure, and the 251 D. odorifera individuals were grouped into five populations including four pure ones (RP1-4) and one mixed one (MIX) based on their maximum membership coefficients. Among these populations, the expected heterozygosity varied from 0.30 (RP3) to 0.38 (RP4). Analysis of molecular variance (AMOVA) showed 11% genetic variation existed among populations, and moderate population differentiation was inferred by the matrix of pairwise Fst (genetic differentiation among populations), which was in the range of 0.031 to 0.095. Moreover, a core collection of 31 D. odorifera individuals including six wild and 25 cultivated trees was developed, which was only 12.4% of the database but conserved the whole genetic diversity. The results of this study provided additional insight into the genetic structure of the large D. odorifera germplasm, and the core collection will be useful for the efficient and sustainable utilization of genetic resources, as well as efficient improvement in breeding programs.

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“…Dalbergia sissoo (shisham) is a multipurpose tree and one of the seven most valuable timber species of India, thus making it an important tree legume for large-scale industrial plantation (Bakshi and Sharma 2011). The bark and wood of D. sissoo are used as an expectorant, antihelminthic, and antipyretic; roots are used as astringent, and leaves are used for eye infection and gonorrhea (Kritikar and Basu 1975).…”

Section: Introductionmentioning

confidence: 99%

“…The bark and wood of D. sissoo are used as an expectorant, antihelminthic, and antipyretic; roots are used as astringent, and leaves are used for eye infection and gonorrhea (Kritikar and Basu 1975). It enriches the soils through nitrogen fixation and with fast decomposing leaves (Bakshi and Sharma 2011). This species also plays an important role in environmental conservation and ecosystem balance (Bari et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Rapid multiplication of Dalbergia sissoo Roxb.: a timber yielding tree legume through axillary shoot proliferation and ex vitro rooting

Vibha

Shekhawat

Mehandru

et al. 2013

Physiol Mol Biol Plants

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An efficient and improved method for in vitro propagation of mature tree of Dalbergia sissoo, an ecologically and commercially important timber yielding species, has been developed through axillary shoot proliferation. Bud breaking occurred from nodal shoot segments derived from rejuvenated shoots produced during early spring from a 20-25-year-old lopped tree, on MS medium containing 8.88 μM benzylaminopurine (BAP). Multiple shoots differentiated (20-21shoots/node) on re-culture of explants on halfstrength agar gelled amended MS medium with a combination of 2.22 μM of BAP and 0.002 μM of thidiazuron (TDZ) with 1.0 mM each of Ca(NO 3 ) 2 , K 2 SO 4 , KCl, and NH 4 (SO 4 ) 2 . The maximum shoot multiplication (29-30 shoots/node) was achieved on subculturing in the above mentioned but liquid medium. Furthermore, the problem of shoot tip necrosis and defoliation observed on solid medium were overcome by the use of liquid medium. Ex vitro rooting was achieved on soilrite after basal treatment of microshoots with 984 μM of indole-3-butyric acid (IBA) for 2 min. About 90 % microshoots were rooted on soilrite within 2-3 weeks under the greenhouse conditions. From 20 nodal shoot segments, about 435 hardened plants were acclimatized and transplanted. This is the first report for rapid in vitro propagation of mature trees of D. sissoo on liquid medium followed by ex vitro rooting.

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Assessment of genetic diversity in Dalbergia sissoo clones through RAPD profiling

Cited by 8 publications

References 6 publications

Unraveling genetic variations of Dalbergia latifolia (Fabaceae) from Yogyakarta and Lombok Island, Indonesia

Unraveling genetic variations of Dalbergia latifolia (Fabaceae) from Yogyakarta and Lombok Island, Indonesia

Genetic Diversity and Population Structure Analysis of Dalbergia Odorifera Germplasm and Development of a Core Collection Using Microsatellite Markers

Rapid multiplication of Dalbergia sissoo Roxb.: a timber yielding tree legume through axillary shoot proliferation and ex vitro rooting

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