Assessment of genetic diversity in Colletotrichum falcatum Went accessions based on RAPD and ISSR markers

Abstract

Sugarcane is susceptible to red rot disease caused by phytopathogenic fungus Colletotrichum falcatum Went which ultimately affect the economy of farmers as well as sugar based industry. One of the various ways to control this devastating disease is to develop disease resistance sugarcane cultivar and this requires the complete understanding of genetic makeup of pathogen. Although South Gujarat is well known sugarcane cultivating area, less published data can be found about PCR-based genetic diversity in prevalent C. falcatum accessions. So, present investigation aims at finding molecular variation among the ten accessions of C. falcatum using RAPD and ISSR molecular markers. A total of 35 RAPD and 39 ISSR primers were screened across 10 C. falcatum accessions, of which 15 RAPD and 21 ISSR primers have showed consistent amplification. Statistics related to genetic variation were estimated using NTSYS-PC by means of Dice’s coefficient. The results revealed 80.6% and 68.07% polymorphism and similarity coefficient ranged from 0.43 to 0.91 and 0.73 to 0.93 in RPAD and ISSR analysis respectively. The dendrogram generated using RAPD, ISSR and combined RAPD-ISSR grouped accessions into different clusters which reveal considerable level molecular variation among the C. falcatum accessions. It is also evident from PCA plots that accessions are rather dispersed with tested marker systems indicating good genetic base. So, in nut shell, we found considerable genetic variation and relatedness within C. falcatum accessions collected from different areas of south Gujarat, India using RAPD and ISSR markers.

Genetic Variability of Isolates of Ramulispora sorghi From Cáceres-MT, Brazil

“…Analyzing the genetic variability of six isolates of Stenocarpella maydis by means of 42 molecular markers of the ISSR type, Fedrigo (2014), obtained a good amplification profile of the primers where PIC values ranged from 0.31 to 0.45 with mean value of 0.36. Patel et al (2018), investigating isolates of Colletotrichum falcatum in sugarcane cultivars found the highest PIC value of 0.84 to 0.46, with an average value of 0.76 for 21 markers of the ISSR type. The results found in all the studies, compared to the results of R. sorghi refer to the ISSR markers the ability to present the information of genetic divergence existing in a population.…”

Genetic diversity using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers in Tinospora cordifolia from the Rayalseema region in Andhra Pradesh

“…Genetic diversity of twelve populations of three mushroom species were evaluated using seven RAPD markers that were able to differentiate the percent variability within and among different populations of these mushroom species (Dwivedi et al, 2018). Sugarcane when genetic diversity was estimated contain similarity coefficient which ranged from 0.43 to 0.91 using thirty five RAPD markers while the ISSR primers could explain the similarity coefficient from 0.73 to 0.93 which is less compared to RAPD markers (Patel et al, 2018). Eighteen synthetic Elite-II hexaploid wheat lines were evaluated using RAPD and SSR based molecular markers and inferred genetic variability of these synthetic lines is due to the novel genetic background derived from Aegilops tauschi accessions used as parental lines (Ahmad, 2014).…”

Developmental, morphological and molecular variation of commercial Ganoderma spp. accessions from southern Vietnam

“…ISSR markers have been successfully used to study genetic composition of different fungus species (Jing et al 2017;Du et al 2018;Patel et al 2018) except Ganoderma. In this study, the potential ability of ISSR markers was used to examine the genetic diversity of nine popular Ganoderma genotypes collected from different provinces in southern Vietnam.…”

“…Among them, ISSR markers are highly preferred because they are based on Polymerase Chain Reaction (PCR) markers and possess common advantages, such as they are simple, rapid and low cost, require minimum laboratory skill, require small DNA quantity, accommodate high number of fragments in each reaction, and do not require prior knowledge of genetic genome of targeted plants. Furthermore, ISSR is a preferred method as a highly variable, reproducible marker (Wolfe and Liston 1998) leading to expanded use in genetic diversity research, population genetic studies, genetic markers, crop identification, source analysis, identification, genetic change identification, and crossbreeding in different fungus species, such as Piptoporus betulinus (Jing et al 2017), Lepista nuda (Du et al 2018), and Colletotrichum falcatum Went (Patel et al 2018) . Nevertheless, ISSR markers have not been commonly used for genetic characterization of Ganoderma.…”