Assessment of genetic diversity of Iranian <i>Ascochyta rabiei</i> isolates using rep‐<scp>PCR</scp> markers

Azizpour, Nadia; Rouhrazi, Kiomars

doi:10.1111/jph.12587

Cited by 4 publications

(3 citation statements)

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“…The genetic diversity of A . rabiei isolates has been assessed using several types of DNA markers, including random amplified polymorphic DNA markers (on 53 isolates from Syria and Lebanon; Udupa et al, 1998 ), sequence‐tagged microsatellite site (STMS) markers (on 36 Australian isolates; Phan et al, 2003b ), amplified fragment length polymorphism, simple‐sequence repeat (SSR) markers (on 64 isolates from the Northwest Plain zone of India; Varshney et al, 2009 ), and rep‐PCR markers (on 29 isolates from Iran; Azizpour & Rouhrazi, 2017 ). However, this limited number of markers on a low number of isolates does not illustrate the true picture of A .…”

Section: Genetic Diversity Population Structure and Emergence Of Aggr...mentioning

confidence: 99%

“…Knowledge about the pathogenic variability and the amount of genetic variation among individual isolates of a country can support the success of germplasm resistance screening programmes, germplasm conservation, chickpea breeding, and prediction of the evolutionary potential of A. rabiei (McDonald & Linde, 2002). The genetic diversity of A. rabiei isolates has been assessed using several types of DNA markers, including random amplified polymorphic DNA markers (on 53 isolates from Syria and Lebanon; Udupa et al, 1998), sequence-tagged microsatellite site (STMS) markers (on 36 Australian isolates; Phan et al, 2003b), amplified fragment length polymorphism, simplesequence repeat (SSR) markers (on 64 isolates from the Northwest Plain zone of India; Varshney et al, 2009), and rep-PCR markers (on 29 isolates from Iran; Azizpour & Rouhrazi, 2017). However, this limited number of markers on a low number of isolates does not illustrate the true picture of A. rabiei diversity and the population structures of a region/country as these markers are not uniformly distributed over the genome.…”

Section: G Ene Ti C D Iver S It Y P Opul Ati On S Truc Ture and Emer...mentioning

confidence: 99%

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Ascochyta rabiei: A threat to global chickpea production

Singh

Kumar

Purayannur

et al. 2022

Molecular Plant Pathology

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The necrotrophic fungus Ascochyta rabiei causes Ascochyta blight (AB) disease in chickpea. A. rabiei infects all aerial parts of the plant, which results in severe yield loss. At present, AB disease occurs in most chickpea‐growing countries. Globally increased incidences of A. rabiei infection and the emergence of new aggressive isolates directed the interest of researchers toward understanding the evolution of pathogenic determinants in this fungus. In this review, we summarize the molecular and genetic studies of the pathogen along with approaches that are helping in combating the disease. Possible areas of future research are also suggested. Taxonomy kingdom Mycota, phylum Ascomycota, class Dothideomycetes, subclass Coelomycetes, order Pleosporales, family Didymellaceae, genus Ascochyta , species rabiei. Primary host A. rabiei survives primarily on Cicer species. Disease symptoms A. rabiei infects aboveground parts of the plant including leaves, petioles, stems, pods, and seeds. The disease symptoms first appear as watersoaked lesions on the leaves and stems, which turn brown or dark brown. Early symptoms include small circular necrotic lesions visible on the leaves and oval brown lesions on the stem. At later stages of infection, the lesions may girdle the stem and the region above the girdle falls off. The disease severity increases at the reproductive stage and rounded lesions with concentric rings, due to asexual structures called pycnidia, appear on leaves, stems, and pods. The infected pod becomes blighted and often results in shrivelled and infected seeds. Disease management strategies Crop failures may be avoided by judicious practices of integrated disease management based on the use of resistant or tolerant cultivars and growing chickpea in areas where conditions are least favourable for AB disease development. Use of healthy seeds free of A. rabiei , seed treatments with fungicides, and proper destruction of diseased stubbles can also reduce the fungal inoculum load. Crop rotation with nonhost crops is critical for controlling the disease. Planting moderately resistant cultivars and prudent application of fungicides is also a way to combat AB disease. However, the scarcity of AB‐resistant accessions and the continuous evolution of the pathogen challenges the disease management process. Useful websites https://www.ndsu.edu/pubweb/pulse‐info/resourcespdf/Ascochyta%20blight%20of%20chickpea.pdf https://saskpulse.com/files/newsletters/180531_ascochyta_in_chickpeas‐compressed.pdf http://www.pulseaus.com.au/growing‐pulses/bmp/chickpea/ascochyta‐blight http://agriculture.vic.gov.au/agriculture/pests‐diseases...

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Section: Genetic Diversity Population Structure and Emergence Of Aggr...mentioning

confidence: 99%

Section: G Ene Ti C D Iver S It Y P Opul Ati On S Truc Ture and Emer...mentioning

confidence: 99%

Ascochyta rabiei: A threat to global chickpea production

Singh

Kumar

Purayannur

et al. 2022

Molecular Plant Pathology

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“…The existence of two mating types of D. rabiei was reported in Syria, Canada, Turkey, Iran, Tunisia, Spain, and USA (Ali et al, 2012;Armstrong et al, 2001;Atik et al, 2011;Azizpour & Rouhrazi, 2017;Bayraktar et al, 2007;Kaiser & Küsmenoglu, 1997;Navas-Cortes et al, 1998; http://dx.doi.org/10.22268/AJPP-038.1.017024 © 2020 Arab Society for Plant Protection ‫النبات‬ ‫لوقاية‬ ‫العربية‬ ‫الجمعية‬ Peever et al, 2004;Rhaiem et al, 2007). In most countries, Mating Type 1 is predominating in the pathogen populations.…”

Section: Introductionmentioning

confidence: 99%

Role of sexual reproduction in the aggressiveness of Didymella rabiei affecting chickpea

Attar¹,

Ahmad²,

Kayim³

et al. 2020

AJPP

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Chickpea production is affected by Ascochyta blight disease in many countries and popular cultivars are put out of production due to the evolution of highly aggressive pathogen population. Sexual reproduction is believed to play an important role in the evolution of aggressive population in Didymella rabiei. Three crosses were made using four parental isolates (AR01 as MAT1-2 and AR02, AR03 and AR04 as MAT1-1) with varying levels of aggressiveness collected from Syria. Twenty randomly selected progenies per cross together with their respective parents were tested on susceptible (ILC-263) and resistant (ICC-12004) chickpea genotypes for their aggressiveness. Moreover, the mating type frequency was determined using multiplex Mating type markers. Latent period and disease severity were used to measure isolate aggressiveness in the progenies. The mean latent period ranged from 6-12 days in all progenies. The progenies showed significant differences on their aggressiveness in the three crosses. High level of aggressiveness of the progenies was generated from AR01 by AR04 crosses. The frequency of the two mating types was almost equal in all crosses. Our findings showed that sexual reproduction can create progenies that can adapt to resistant cultivars and could affect the effectiveness of commonly used fungicides to manage Ascochyta blight.

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