Assessment of genetic fidelity of micropropagated date palm (Phoenix dactylifera L.) plants by RAPD and ISSR markers assay

Kumar, Nitish; Modi, Arpan; Singh, Amritpal S.; Gajera, Bhavesh; Patel, Armi; Patel, M. P.; Subhash, Narayanan

doi:10.1007/s12298-010-0023-9

Cited by 54 publications

(21 citation statements)

References 21 publications

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“…RAPD has been successfully applied in genetic variation investigations of Mucuna pruriens due to its usefulness as an informative and cheap tool for plant breeding programs (Patil et al 2016). There are also many researchers using these markers as one of their techniques to study the genetic diversity of their desired plant species (Desai et al 2015;Kumar et al 2009Kumar et al , 2010Mei et al 2015;Patel et al 2015;Patil et al 2016;Pu et al 2009;Saleh 2015;Zhao et al 2013;Zhao et al 2015). RAPD and ISSR are dominant markers that function well in DNA fingerprinting studies of plant species.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of genetic diversity of Clinacanthus nutans (Acanthaceaea) using RAPD, ISSR and RAMP markers

Ismail

Arsad

Samian

et al. 2016

Physiol Mol Biol Plants

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Three polymerase chain reaction (PCR) techniques were compared to analyse the genetic diversity of Clinacanthus nutans eight populations in the northern region of Peninsular Malaysia. The PCR techniques were random amplified polymorphic deoxyribonucleic acids (RAPD), inter-simple sequence repeats (ISSR) and random amplified microsatellite polymorphisms (RAMP). Leaf genomic DNA was PCR amplified using 17 RAPD, 8 ISSR and 136 RAMP primers . However, only 10 RAPD primers, 5 ISSR primers and 37 RAMP primers produced reproducible bands. The results were evaluated for polymorphic information content (PIC), marker index (MI) and resolving power (RP). The RAMP marker was the most useful marker compared to RAPD and ISSR markers because it showed the highest average value of PIC (0.25), MI (11.36) and RP (2.86). The genetic diversity showed a high percentage of polymorphism at the species level compared to the population level. Furthermore, analysis of molecular variance revealed that the genetic diversity was higher within populations, as compared to among populations of C. nutans. From the results, the RAMP technique was recommended for the analysis of genetic diversity of C. nutans.

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Section: Introductionmentioning

confidence: 99%

Evaluation of genetic diversity of Clinacanthus nutans (Acanthaceaea) using RAPD, ISSR and RAMP markers

Ismail

Arsad

Samian

et al. 2016

Physiol Mol Biol Plants

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“…Molecular marker techniques like Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) are rapid, sensitive and reliable in analysis of genetic stability of in vitro regenerants. These two marker systems have been successfully applied to detect the somaclonal variations in a number of species such as Cymbopogon martini (Bhattacharya et al 2010), Phoenix dactylifera (Kumar et al 2010) and Simmondsia chinensis (Kumar et al 2011). …”

Section: Introductionmentioning

confidence: 99%

Micropropagtion of Terminalia bellerica from nodal explants of mature tree and assessment of genetic fidelity using ISSR and RAPD markers

Dangi

Khurana-Kaul

Kothari

et al. 2014

Physiol Mol Biol Plants

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The present study reports an efficient in vitro micropropagation protocol for a medicinally important tree, Terminalia bellerica Roxb. from nodal segments of a 30 years old tree. Nodal segments taken from the mature tree in MarchApril and cultured on half strength MS medium gave the best shoot bud proliferation response. )] in the culture medium aided to minimize browning and improve explant survival during shoot bud induction. Highest multiplication of shoots was achieved on medium supplemented with 6-benzyladenine (BA, 8.8 μM) and α-naphthalene acetic acid (NAA, 2.6 μM) in addition to antioxidants. Shoot elongation was obtained on MS medium containing BA (4.4 μM)+phloroglucinol (PG, 3.9 μM). Elongated shoots were transferred to half strength MS medium containing indole-3-butyric acid (IBA, 2.5 μM) for root development. The acclimatization of plantlets was carried out under greenhouse conditions. The genetic fidelity of the regenerated plants was checked using inter simple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD) analysis. Comparison of the bands among the regenerants and mother plant confirmed true-to-type clonal plants.

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“…In commercial micropropagation it is very important to test the genetic conformity/true-to-type of the regenerated plants. Several researchers have used molecular markers (AFLP, ISSR and RAPD) to confirm the genetic conformity of the tissue-cultured plants (Kumar et al, 2010;Othmani et al, 2009cOthmani et al, , 2010. The use of RAPD primers showed micropropagated date cv.…”

Section: Molecular Characterizationmentioning

confidence: 99%

“…Barhee and Khalas which display dwarfism and abnormal flower development (Al-Kaabi;Zaid;Ainsworth, 2007;Zaid;Al-Kaabi, 2003). Kumar et al (2010) evaluated 27 micropropagated date plants with 160 RAPD and 21 ISSR primers, in which 30 RAPD and 12 ISSR primers produced a total of 347 reproducible monomorphic band; the results suggested that the micropropagated plants are true-to-type. Aslam, Khan and Naqvi (2015) established the somatic embryo derived regeneration protocol for the six cvs.…”

Section: Molecular Characterizationmentioning

confidence: 99%

Date palm micropropagation: Advances and applications

Al-Khayri

Naik

2017

Ciênc. agrotec.

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Date palm (Phoenix dactylifera L.) is a fruit tree resilient to adverse climatic conditions predominating in hot arid regions of the Middle East and North Africa. The date fruit contains numerous chemical components that possess high nutritional and medicinal values. Traditional propagation by offshoots is inefficient to satisfy current demands for date palm trees. Alternatively, micropropagation provides an efficient means for large-scale propagation of date palm cultivars. Both somatic embryogenesis and organogenesis, either directly or indirectly though the callus phase, have been demonstrated in date palm in vitro regeneration. Culture initiation commonly utilizes shoot-tip explants isolated from young offshoots. Recently, the immature inflorescences of adult trees were utilized as an alternative nondestructive source of explants. In addition to the nature of the explant used, successful plant regeneration depends on the cultivar, composition of the culture medium and physical status. Challenges of date palm micropropagation include long in vitro cycle, latent contamination, browning, somaclonal variation as well as ex vitro acclimatization and transplanting. A remarkable amount of research investigating these factors has led to optimized protocols for the micropropagation of numerous commercially important cultivars. This has encouraged the development of several international commercial tissue culture laboratories. Molecular characterization provides an assurance of genetic conformity of regenerated plantlets, a key feature for commercial production. This article describes date palm micropropagation protocols and also discusses recent achievements with respect to somaclonal variation, molecular markers, cryopreservation and future prospects.Index terms: Cryopreservation; somatic embryogenesis; somaclonal variation; organogenesis; molecular marker. RESUMOA tamareira (Phoenix dactylifera L.) é uma arvore frutífera adaptada à condições climáticas adversas predominantemente em regiões áridas do Oriente Médio e Norte Africano. As tâmaras possuem vários componentes químicos com alto valor medicinal e nutricional. A propagação tradicional por estacas não é suficiente para satisfazer a demanda por mudas e assim, a micropropagação apresenta-se como uma alternativa eficiente para a produção de mudas em larga escala. Embriogênese somática e organogênese, tanto direta quanto indireta via calos, tem sido usada para obter a regeneração in vitro de tamareira. O inicio do cultivo in vitro normalmente utiliza meristemas excisados de brotações jovens. Recentemente, inflorescências imaturas de árvores adultas são usadas como fonte alternativa de explantes não destrutiva. Além da origem do explante, o sucesso da regenerção depende do cultivar, da composição do meio de cultura e de condições físicas. Desafios na micropropagação de tamareira incluem um longo ciclo in vitro, contaminação, escurecimento do tecido, variação somaclonal além do enraizamento e aclimatização ex vitro. Diversos estudos investigando esses fatore...

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Assessment of genetic fidelity of micropropagated date palm (Phoenix dactylifera L.) plants by RAPD and ISSR markers assay

Cited by 54 publications

References 21 publications

Evaluation of genetic diversity of Clinacanthus nutans (Acanthaceaea) using RAPD, ISSR and RAMP markers

Evaluation of genetic diversity of Clinacanthus nutans (Acanthaceaea) using RAPD, ISSR and RAMP markers

Micropropagtion of Terminalia bellerica from nodal explants of mature tree and assessment of genetic fidelity using ISSR and RAPD markers

Date palm micropropagation: Advances and applications

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