Assessment of Genetic Purity of Inter-Specific F1 Hybrids Involving Vigna Radiata and Vigna Umbellata

Abstract

Genetic purity test of true hybrids from controlled crosses before further generations of selfing or crossing and selection is essential for Mungbean improvement. The present study was conducted to transfer mungbean yellow mosaic (MYM) disease resistance in mungbean from ricebean and to assess the genetic purity of developed inter-specific F1 hybrids using morphological features and microsatellite markers. Inter-specific crosses were made involving two genotypes of mungbean viz., K 851 and TM 96-2 and one genotype of ricebean (RBL 1) where the crossability results revealed significant differences. Crossability was recorded 8.2% (TM 96-2 × RBL 1) and 4.6% (K 851 × RBL 1). Pollen fertility was recorded 1.6% and 3.4% in TM 96-2 × RBL 1 and K 851 × RBL 1, respectively. Morphological features such as epicotyl colour, hypocotyl length, petiole length, germination habit, etc., were used as indicators of true hybridity. Further, the microsatellite markers were used to confirm the genetic purity of the developed inter-specific hybrid. These hybrids exhibited resistance against mungbean yellow mosaic disease under natural epiphytotic field conditions. The present study will be useful in developing high yielding varieties or lines of mungbean coupled with stable MYMV resistance.

Introgression of disease resistance in urdbean (Vigna mungo) by involving its related species

“…VR0200. Bhanu et al (2017) also confirmed the hybridity of V. radiata × V. umbellata interspecific hybrids using morphological and adzukibean specific SSR markers. Abbas et al (2015) used SSR and RAPD markers to confirm the hybridity of interspecific hybrids between moongbean and urdbean.…”

Breeding for Enhancing Legumovirus Resistance in Mungbean: Current Understanding and Future Directions

“…Some of the researchers have successfully studied the gene flow from wild to cultivated Vigna, and they have noticed that there is enough opportunity to facilitate gene transfer. The resistant gene of YMD has been successfully incorporated into urdbean [175] and mungbean [176,177]. Likewise, Mathivatahana et al [178] used rice bean as a donor for MYMV-resistance in mungbean.…”

Interspecific hybridization and crossability studies of cultivated varieties of Vigna mungo L. Hepper with Vigna mungo var silvestris

“…The crosses were attempted by using black gram cultivars as the female parents as success rate was higher in these crosses as reported by Biswas and Dana (1975) The pollen fertility of the parents ranged from 92.88 to 94.25 per cent. Bhanu et al (2017) recorded a pollen fertility of 1.6 to 3.4 per cent for the hybrids obtained from the green gram x rice bean cross. The fertility status of the parents and hybrids are presented in Table 4 and Fig.…”