Assessment of genetic variability among rabbit breeds by random amplified polymorphic DNA (RAPD)-PCR.

Rangoju, P K; Kumar, Sushil; Kolte, Atul P.; Gulyani, R.; Singh, V. K.

doi:10.4995/wrs.2007.611

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…Rangoju et al [10] used RAPD markers to assess the genetic variability among three rabbit breeds: Soviet Chinchilla, White Giant, and Grey Giant. They reported no significant genetic variability among and within these three rabbit breeds.…”

Section: Introductionmentioning

confidence: 99%

Origin, Genetic Diversity, and Population Structure of Rabbits (Oryctolagus cuniculus) in Kenya

Owuor

Mamati

Kasili

2019

BioMed Research International

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To evaluate the origin, genetic diversity, and population structure of domesticated rabbits in Kenya, a 263-base pair region of mtDNA D-loop region of 111 rabbits sampled from Kakamega, Vihiga, and Bungoma counties in the western region, Laikipia and Nyandarua counties in the central region, and Kitui, Machakos, and Makueni in the eastern region of the country were analyzed. The average haplotype (0.40702) and nucleotide (0.01494) diversities observed were low, indicating low genetic diversity of domesticated rabbits in Kenya. This study resolved 5 unique haplotypes in the mtDNA D-loop region. A population genetic structure distinguishing Europe grouping and domesticated rabbits in Kenya was obtained on incorporating 32 known haplotypes. Domesticated rabbits in Kenya clustered together with rabbits from other geographic regions, suggesting common origin. The results suggested that the Kenyan domesticated rabbits may have originated from Europe. Integration of exotic breeds into breeding programmes could have contributed to the low genetic diversity. These results provide useful information for breeding and conservation decisions by the relevant stakeholders in the agriculture industry in Kenya.

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Section: Introductionmentioning

confidence: 99%

Origin, Genetic Diversity, and Population Structure of Rabbits (Oryctolagus cuniculus) in Kenya

Owuor

Mamati

Kasili

2019

BioMed Research International

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“…In their study, all primers produced polymorphic bands in the range of 5 to 11. Rangoju et al, (2007) assessed genetic variability and phylogenetic relationship among three rabbit breeds using six random primers (OPA-01, OPA-08, OPA-10 ,OPA-18 , OPB-03 and OPB-05). They found that, the maximum number of bands was (13.2 ± 0.4), while minimum number of bands were (6.4 ± 0.2) in all the breeds.…”

Section: Resultsmentioning

confidence: 99%

Assessment of Genetic Variability among Some Rabbit Breeds Using Random Amplified Polymorphic DNA Technique (RAPD)

2015

Annals of Agricultural Science, Moshtohor

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Random Amplified Polymorphic DNA (RAPD) marker was employed to assess the genetic variation and phylogenetic relationship among four rabbit breeds viz. New Zealand White, Gabali, Baladi Red and Baladi Black reared in Egypt. Initially, a total of 14 random primers of arbitrary sequence were used but 10 of them generated reproducible, scoreable and polymorphic bands. Out of 131 bands scored using these primers, 74(56.48%) were recognized as Polymorphic and 57 (43.52%) as monomorphic bands. The highest percentage of polymorphic bands was recognized for primers OPB-02 (94%) and OPB-07 (92%). While, the lowest percentage of polymorphic bands was recognized for primers OPA-02(16%) and OPF-12 (28%). The band sharing frequencies (BSF) was found higher between Baladi Red-Baladi Black (0.80± 0.038) , followed by Gabali-Baladi Black (0.71±0.079), New Zealand White-Baladi Black (0.70±0.096), New Zealand Whit-Baladi Red (0.69±0.088)and the least BSF was found between New Zealand Whit-Gabali (0.64±0.081). Overall, there was no significant difference (P>0.05) in BSF values between breeds. The highest genetic distance was found between Baladi Red-Baladi Black (0.87) followed by Gabali, Baladi Red (0.86), Gabali, Baladi Black(0.82), New Zealand White, Gabali (0.80), New Zealand White,Baladi Red (0.75) and the lowest genetic distance was found between New Zealand White,Baladi Black(0.73). One primer (OPA-20) in Gabali , two primers(OPA-02,OPB-14) in Baladi Red, three primers (OPA-02,OPB-14, OPA-20) in New Zealand White and Baladi Black were found to be specific for these breeds. The study suggests that RAPD can be successfully utilized for detecting genetic variation among the studied rabbit breeds.

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“…This increases the potential that they represent complex species. The assessment of the genetic variation of a species is an important gauge for its preservation, maintenance, and further genetic improvement [19] . The population genetic structure is due to many factors like ecological and geographical, which may lead to divergence and division of the populations.…”

Section: Introductionmentioning

confidence: 99%

Phylogeographic and Genetic Diversity of Porcellionides Pruinosus and Porcellio Laevis by Using the Mitochondrial Cytochrome C Oxidase Subunit 1 Sequence

Allam

Fangary

Marie

2021

Egyptian Journal of Zoology

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Among Oniscidea suborder, Porcellionidae is the richest and widely-distributed Family. However, the phylogeny investigations of its taxa were not completely covered. Therefore, a phylogeographic population and genetic diversity study was carried out here on two cosmopolitan terrestrial isopod species (Porcellionides pruinosus and Porcellio laevis) by utilizing mitochondrial cytochrome c oxidase subunit 1 (CO1) gene. The sequencing of CO1 among the understudied populations showed nucleotide length ranged from 646 base pairs (bp) to 660 bp. The average contents of nucleotide bases A+T in Porcellionides pruinosus and Porcellio laevis were 63.8% and 63.3%, respectively. Also, the average content of pyrimidines (C+T) bases in Porcellionides pruinosus and Porcellio laevis was higher than the purines content. Nucleotide bases A+T contents were higher than nucleotide bases C+G contents in all populations. The pairwise genetic distances among the understudied Porcellionides pruinosus populations ranged from 0.000 to 0.002. The most related populations were in Qeft and Deshna, where the genetic distance was 0.000. The pairwise genetic distances among the understudied populations of Porcellio laevis ranged from 0.017 to 0.033. The most related populations were in Qeft and Hurghada, where the genetic distance was 0.017. Furthermore, Porcellio laevis populations need more investigations, as it seems to be polyphyletic comparing to Porcellionides pruinosus populations.

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Assessment of genetic variability among rabbit breeds by random amplified polymorphic DNA (RAPD)-PCR.

Cited by 3 publications

References 14 publications

Origin, Genetic Diversity, and Population Structure of Rabbits (Oryctolagus cuniculus) in Kenya

Origin, Genetic Diversity, and Population Structure of Rabbits (Oryctolagus cuniculus) in Kenya

Assessment of Genetic Variability among Some Rabbit Breeds Using Random Amplified Polymorphic DNA Technique (RAPD)

Phylogeographic and Genetic Diversity of Porcellionides Pruinosus and Porcellio Laevis by Using the Mitochondrial Cytochrome C Oxidase Subunit 1 Sequence

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