Assessment of genetic variability and molecular characterization of heat stress tolerant genes in Arachis hypogaea L. through qRT-PCR

Kokkanti, Rekha Rani; Vemuri, Hindu; Latha, P.; Vasanthi, R.P.; Sudhakar, P.; Usha, R.

doi:10.1016/j.bcab.2019.101242

Cited by 7 publications

(4 citation statements)

References 38 publications

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“…Plant heat shock factors (HSFs) play a key role in groundnut response to various environmental stresses by regulating the expression of stress responsive genes, such as heat shock proteins (HSPs), dehydration responsive element-binding proteins (DREBs), late embryogenesis abundant proteins (LEA), and abscisic acid response element-binding proteins AREB [ 130 ]. CRT element-binding factors (CBFs) were also found to respond to various plant stresses [ 131 , 132 , 133 , 134 ].…”

Section: The Exploitation Of the Cwrs In Specific Cultivated Speciesmentioning

confidence: 99%

Crop Wild Relatives: A Valuable Source of Tolerance to Various Abiotic Stresses

Kapazoglou¹,

Gerakari

Lazaridi

et al. 2023

Plants

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Global climate change is one of the major constraints limiting plant growth, production, and sustainability worldwide. Moreover, breeding efforts of the past years have focused on improving certain favorable crop traits, leading to genetic bottlenecks. The use of crop wild relatives (CWRs) to expand genetic diversity and improve crop adaptability seems to be a promising and sustainable approach for crop improvement in the context of the ongoing climate challenges. In this review, we present the progress that has been achieved towards CWRs exploitation for enhanced resilience against major abiotic stressors (e.g., water deficiency, increased salinity, and extreme temperatures) in crops of high nutritional and economic value, such as tomato, legumes, and several woody perennial crops. The advances in -omics technologies have facilitated the elucidation of the molecular mechanisms that may underlie abiotic stress tolerance. Comparative analyses of whole genome sequencing (WGS) and transcriptomic profiling (RNA-seq) data between crops and their wild relative counterparts have unraveled important information with respect to the molecular basis of tolerance to abiotic stressors. These studies have uncovered genomic regions, specific stress-responsive genes, gene networks, and biochemical pathways associated with resilience to adverse conditions, such as heat, cold, drought, and salinity, and provide useful tools for the development of molecular markers to be used in breeding programs. CWRs constitute a highly valuable resource of genetic diversity, and by exploiting the full potential of this extended allele pool, new traits conferring abiotic-stress tolerance may be introgressed into cultivated varieties leading to superior and resilient genotypes. Future breeding programs may greatly benefit from CWRs utilization for overcoming crop production challenges arising from extreme environmental conditions.

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Section: The Exploitation Of the Cwrs In Specific Cultivated Speciesmentioning

confidence: 99%

Crop Wild Relatives: A Valuable Source of Tolerance to Various Abiotic Stresses

Kapazoglou¹,

Gerakari

Lazaridi

et al. 2023

Plants

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“…In another study, peanut seedlings of diverse genotypes for heat tolerance were screened using temperature induction response techniques. About 2 days old peanut seedlings were exposed to sublethal temperature from 28°C to 54°C for 5 h, followed by the lethal temperature at 54°C for 3 h. The expression patterns of stress-responsive genes were analyzed in selected heat-tolerant genotypes; genes related to HSP90, DREB2A, and LEA4-2 were highly induced ( Kokkanti et al, 2019 ) that can be used as markers for screening. Lipid peroxidation can cause severe membrane injury (MI) during abiotic stress.…”

Section: High-throughput Phenomics To Accelerate Data Collection In G...mentioning

confidence: 99%

“…Genes related to HSP90, dehydration-responsive element binding -2A (DREB2A), and late embryogenesis abundant 4-2 (LEA4-2) were highly induced during heat stress in a few peanut genotypes ( Kokkanti et al, 2019 ). Heat stress generates ROS, such as superoxide radicals and H 2 O 2, which results in oxidative damage to biomolecules, lipid peroxidation, and reduced activities of ROS-scavenging enzymes ( Dat et al, 2000 ).…”

Section: Molecular Basis Of Stress Tolerancementioning

confidence: 99%

Sustaining yield and nutritional quality of peanuts in harsh environments: Physiological and molecular basis of drought and heat stress tolerance

et al. 2023

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Climate change is significantly impacting agricultural production worldwide. Peanuts provide food and nutritional security to millions of people across the globe because of its high nutritive values. Drought and heat stress alone or in combination cause substantial yield losses to peanut production. The stress, in addition, adversely impact nutritional quality. Peanuts exposed to drought stress at reproductive stage are prone to aflatoxin contamination, which imposes a restriction on use of peanuts as health food and also adversely impact peanut trade. A comprehensive understanding of the impact of drought and heat stress at physiological and molecular levels may accelerate the development of stress tolerant productive peanut cultivars adapted to a given production system. Significant progress has been achieved towards the characterization of germplasm for drought and heat stress tolerance, unlocking the physiological and molecular basis of stress tolerance, identifying significant marker-trait associations as well major QTLs and candidate genes associated with drought tolerance, which after validation may be deployed to initiate marker-assisted breeding for abiotic stress adaptation in peanut. The proof of concept about the use of transgenic technology to add value to peanuts has been demonstrated. Advances in phenomics and artificial intelligence to accelerate the timely and cost-effective collection of phenotyping data in large germplasm/breeding populations have also been discussed. Greater focus is needed to accelerate research on heat stress tolerance in peanut. A suits of technological innovations are now available in the breeders toolbox to enhance productivity and nutritional quality of peanuts in harsh environments. A holistic breeding approach that considers drought and heat-tolerant traits to simultaneously address both stresses could be a successful strategy to produce climate-resilient peanut genotypes with improved nutritional quality.

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“…The standardized normal Z distribution is an efficient tool employed to cluster the genotypes/ RIL's into different groups (susceptible and tolerant) based on the percent seedling survival and the percent reduction in recovery growth under induction temperature over the control (Srikanthbabu et al, 2002;Bhavana et al, 2019;Kokkanti et al, 2019;Chaudhary et al, 2020). The RIL's having a higher seedling survival with a lesser percent reduction in recovery growth are considered tolerant; and the RIL's with a lower seedling survival percentage and having a higher percent reduction in recovery growth are considered susceptible to high temperature (Figure 2).…”

Section: Identification Of Contrasting Ril's Based On Tir Studymentioning

confidence: 99%

In Vitro Selection of Recombinant Inbred Lines for Thermotolerance Using Temperature Induction Response in Finger Millet (Eleusine coracana L.)

Y A

2023

MAJ

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Climate change is leading to rising global temperatures, causing shifts in weather patterns and more frequent extreme heat events. These temperature shifts disrupt plant growth and development, threatening global ecosystems and food security. Finger millet is a climate-resilient crop, gaining attention for its relative ability to tolerate high temperatures and drought conditions compared to other cereal food crops, but yield improvement is stagnated. The newer sources of stress tolerance in finger millet can be obtained through the development of Recombinant Inbred Lines (RIL’s). In the present study, 222 RIL’s (F6) developed for thermotolerance were screened using Temperature induction response (TIR). The study revealed wide variability in the seedling traits such as seedling survival (%), reduction in the shoot, root, and total seedling length (%), as well as the seedling vigour index (SVI). By employing the standardized Z distribution, the RIL’s were categorized into tolerant and susceptible based on the percent seedling survival and percent reduction in recovery growth. Five RIL’s viz. 6.1.11, 6.5.10, 6.12.5, 6.13.8, and 6.20.24 were identified as tolerant while five viz. 6.3.2, 6.4.12, 6.7.2, 6.10.14 and 6.17.8a were recognized as susceptible. The identified RILs can be further used as donor source for crop improvement studies

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Assessment of genetic variability and molecular characterization of heat stress tolerant genes in Arachis hypogaea L. through qRT-PCR

Cited by 7 publications

References 38 publications

Crop Wild Relatives: A Valuable Source of Tolerance to Various Abiotic Stresses

Crop Wild Relatives: A Valuable Source of Tolerance to Various Abiotic Stresses

Sustaining yield and nutritional quality of peanuts in harsh environments: Physiological and molecular basis of drought and heat stress tolerance

In Vitro Selection of Recombinant Inbred Lines for Thermotolerance Using Temperature Induction Response in Finger Millet (Eleusine coracana L.)

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