Assessment of genetic variability of olive varieties by microsatellite and AFLP markers

Bandelj, Dunja; Jakše, Jernej; Javornik, Branka

doi:10.1023/b:euph.0000019552.42066.10

Cited by 94 publications

(74 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Common genetic background was confirmed for all Tuscan olive varieties for oil use. The clustering analysis with new genic markers confirmed that Slovenian varieties "Zelenjak" and "Črnica" are closed related with Tuscan olives, as previously noted (Bandelj et al, 2004). Both genomic and functional EST-SSRs also showed that the predominant local variety "Istrska belica" had very low genetic similarities with other varieties.…”

Section: Discussionsupporting

confidence: 78%

“…Averages for H o and H e were 0.649 and 0.604, respectively. Slightly greater results for observed and expected heterozygosity (H o = 0.769, H e = 0.705) were obtained in a study, where 19 varieties, also used in this study, were tested with genomic SSRs (Bandelj et al, 2004). The comparable ability of genomic and EST-SSRs in the detection of genetic diversity in olives was also confirmed by De la .…”

Section: Discussionsupporting

confidence: 71%

“…The first cluster, which showed higher genetic similarities with respect to the other groups, contained all Tuscan varieties for oil use ("Leccino", "Leccio del corno", "Pendolino", "Leccione, "Maurino", "Frantio"). This cluster also contained the Italian variety "Coratina" as well as "Athena' and "Zelenjak", which were probably brought from central Italy to the northern Adriatic coast as shown in a previous study (Bandelj et al, 2004). "Črnica" and "Štorta" were also close to the Tuscan group, which are known as Slovenian varieties for oil production and table use, re- spectively.…”

Section: Polymorphism and Genetic Diversity Analysissupporting

confidence: 57%

“…Comparing results of the clustering analysis with those obtained by genomic SSRs (Bandelj et al, 2004) revealed a similar distribution of olive varieties in related groups. Common genetic background was confirmed for all Tuscan olive varieties for oil use.…”

Section: Discussionmentioning

confidence: 64%

See 3 more Smart Citations

Identification and validation of novel EST-SSR markers in olives

Arbeiter

Hladnik

Jakše

et al. 2017

Sci. agric. (Piracicaba, Braz.)

Self Cite

View full text Add to dashboard Cite

The olive (Olea europaea L.) is a leading oil crop in the Mediterranean area. Limited information on the inheritance of agronomic significant traits hinders progress in olive breeding programs, which encourages the development of markers linked to the traits. In this study, we report on the development of 46 olive simple sequence repeat (SSR) markers, obtained from 577,025 expressed sequence tags (ESTs) in developing olive fruits generated in the framework of the Slovenian national olive transcriptome project. Sequences were de novo assembled into 98,924 unigenes, which were then used as a source for microsatellites searching. We identified 923 unigenes that contained 984 SSRs among which dinucleotide SSRs (36 %) were the most abundant, followed by tri-(33 %) and hexa-(21 %) nucleotides. Microsatellite repeat motif GA (37 %) was the most common among dinucleotides, while microsatellite repeat motif GAA was the most abundant trinucleotide SSR motif (16 %). Gene ontology annotations could be assigned to 27 % of the unigenes. A hundred and ten expressed sequence tag-derived-simple sequence repeats (EST-SSRs) with annotated genes were selected for primer designing and finally, 46 (42 %) polymorphic EST-SSRs were successfully amplified and used to validate genetic diversity among 24 olive varieties. The average number of alleles per locus, observed heterozygosity, expected heterozygosity, and polymorphic information content were 4.5, 0.649, 0.604 and 0.539, respectively. Twenty-seven EST-SSRs showed good diversity properties and were recommended for further olive genome investigation.

show abstract

Section: Discussionsupporting

confidence: 78%

Section: Discussionsupporting

confidence: 71%

Section: Polymorphism and Genetic Diversity Analysissupporting

confidence: 57%

Section: Discussionmentioning

confidence: 64%

See 2 more Smart Citations

Identification and validation of novel EST-SSR markers in olives

Arbeiter

Hladnik

Jakše

et al. 2017

Sci. agric. (Piracicaba, Braz.)

Self Cite

View full text Add to dashboard Cite

show abstract

“…At the molecular level, our results ( Tables 1, 2) and comparable studies in the literature presented high polymorphism ratio (70.2% in 9 RAPD primers) among fig genotypes grown in the Mediterranean countries which commonly ranged between 39% -81% within the same marker (39% in 12 RAPD primers [14]; 67% in 7 RAPD primers [19]; 72% in 6 RAPD primers [10]; 70% in 13 RAPD primers [30]; 77% in 6 RAPD primers [7]; 81% in 7 RAPD primers [21]). Indeed, the high polymorphism that was observed here is indicative of more divergent genotypes, and consequently a potential for success in future selection programs [31]. Additionally, the average of 6.33 amplicons (loci) per primer presented in this study ( Table 2) was sufficient to produce useful fin-gerprints for genotype and clone discrimination [14,17].…”

Section: Discussionmentioning

confidence: 88%

Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.)

Basheer-Salimia

Awad²,

Ward³

2012

AJPS

View full text Add to dashboard Cite

Both morphological characters and PCR-based RAPD approaches were used to determine the genetic diversity and relatedness among nine fig genotypes grown at the northern region of the West-Bank, Palestine. Although we tested 28 primers for the RAPD technique, only 9 produced reasonable amplification products. A total of 57 DNA loci were detected in which 70.2% were polymorphic. DNA fragments presented a minimum of 3 and a maximum of 9 polymorphic bands using primers OPT-10 and OPA-18, respectively. Primers exhibited collective resolving power values (Rp) of 18.826. The Mwazi genotype showed the highest genetic distances among all of the other genotypes. Morphologically, considerable variations were found using 41 quantitative and qualitative traits. Adloni could be a very promising genotype for fresh consumption due to its very late maturation period, extended harvesting period, variable fruit size, and easy skin peeling. In addition, 7 genotypes presented firm fruits, which are a very important criterion for exporting purposes. Dendrogram constructed by UPGMA based on RAPD banding patterns appear somewhat contradictory to the morphological descriptors particularly with Swadi and Biadi genotypes (closed genetically and distanced morphologically), which might be attributed to the phenotypic modifications caused by environmental differences across regions. These preliminary results will make a fundamental contribution to further genetic improvement of fig crops for the region.

show abstract