Assessment of Genetic Variation in Different Kenaf (Hibiscus cannabinus) Genotypes using Morpho-agronomic Traits and RAPD Markers

Abstract

To cite this paper: Faruq, G., M.M. Rahman, H. Zabed and A. Latif, 2015. Assessment of genetic variation in different kenaf (Hibiscus cannabinus) genotypes using morpho-agronomic traits and RAPD markers. AbstractTwenty five kenaf (Hibiscus canabinus L.) genotypes originated in different parts of the world were studied in Malaysian tropical environment to assess genetic variation using morpho-agronomic traits and random amplified polymorphic DNA (RAPD) markers. A total of 13 morpho-agronomic traits was selected for study and found that the genotypes varied significantly (p<0.01) in these traits. The major production traits such as stick weight (SW) and fiber weights (FW) were found highly correlated with other traits. Agglomerative hierarchical cluster analysis and principal component analysis (PCA) of the morpho-agronomic traits yielded four major cluster groups of the studied genotypes. The low fiber and stick yield producing early maturing genotypes were in cluster I, middle fiber weight, plant height and matured genotypes were in cluster II, and III and high fiber and stick weight producing late flowering genotypes were in cluster IV PCA of the phenotypic data using covariance matrix revealed that first three components accounted for 97.20% of total variation of the genotypes. The assessment of genetic diversity using RAPD marker revealed high genetic polymorphisms of the makers (7.2) with Jaccard's similarity coefficient of variation from 0.000 to 0.952. These findings of phenotypic and genetic variations in morphoagronomic traits and polymorphism level at DNA expressed the usefulness of these genotypes as parent materials for future improvement in kenaf breeding programs.

Comparative cytogenetics of kenaf (Hibiscus cannabinusL.) breeding lines reveal chromosomal variability and instability

“…Hence, regarding the assessment of kenaf’s molecular diversity, most studies to date rely on molecular markers. These encompass simple and inter-simple sequence repeats (SSR, ISSR; Tao et al 2005; Satya et al 2013), randomly amplified polymorphic DNA (RAPD; Cheng et al 2002; Faruq et al 2015), amplified fragment length polymorphism (AFLP; Cheng et al 2004; Coetzee et al 2008; Kim et al 2010), sequence-related amplified polymorphism (SRAP; Tao et al 2005; Qi et al 2011; Zhang et al 2011), and single nucleotide polymorphism (SNP) markers (Zhang et al 2020). Nevertheless, kenaf breeding is still impaired by the lack of genetic understanding of the kenaf germplasm and native breeding resources.…”

Agronomic Behavior of Mexican Roselle Cultivars Produced under Protected Agricultural Conditions

“…The generalized method for defining the variability of roselle cultivars is the study of morpho–agronomic traits because the morphological characteristics determine its classification [ 25 ]. Direct measurements of growth, height, leaf area and cultivation time are used for the quantitative analysis of plant growth and for the calculation of crop growth rates of commercial importance [ 26 ].…”