Assessment of genotoxic effects by Lindane

Pool‐Zobel, Beatrice L.; Guigas, Claudia; Klein, R.G.; Neudecker, C.; Renner, H.W.; Schmezer, Peter

doi:10.1016/0278-6915(93)90077-c

Cited by 148 publications

(68 citation statements)

References 16 publications

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“…The lymphocytes were checked for their viability before the start and after the end of the reaction using Trypan Blue Exclusion test [24]. The viability of the cells was found to be greater than 93%.…”

Section: Viability Assessment Of Lymphocytesmentioning

confidence: 99%

Resveratrol–Cu(II) induced DNA breakage in human peripheral lymphocytes: Implications for anticancer properties

2005

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Resveratrol (3,40 ,5-trihydroxy stilbene), a plant derived polyphenol found in mulberries, grapes and red wine is considered to possess chemopreventive properties against cancer. It is recognized as a naturally occurring antioxidant but also catalyzes oxidative DNA degradation in vitro in the presence of transition metal ions such as copper. Using a cellular system of lymphocytes isolated from human peripheral blood and Comet assay, we have confirmed that resveratrol-Cu(II) system is indeed capable of causing DNA degradation in cells such as lymphocytes. Also, trans-stilbene, which does not have any hydroxyl groups, is inactive in the lymphocyte system. Pre-incubation of lymphocytes with resveratrol indicates that it is capable of either traversing the cell membrane or binding to it. Our results are in partial support of our hypothesis that anticancer properties of various plant derived polyphenols may involve mobilization of endogenous copper and the consequent prooxidant action.

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Section: Viability Assessment Of Lymphocytesmentioning

confidence: 99%

Resveratrol–Cu(II) induced DNA breakage in human peripheral lymphocytes: Implications for anticancer properties

2005

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“…Also, effects of allicin, if any, were probed by analyzing the housekeeping genes like b-actin by reverse transcription (RT)-PCR and R18 by quantitative real-time RT-PCR. In addition, viability was also ascertained using trypan blue exclusion test [13].…”

Section: Viability Assessment Of Monocytesmentioning

confidence: 99%

Suppression of Mycobacterium tuberculosis induced reactive oxygen species (ROS) and TNF‐α mRNA expression in human monocytes by allicin

et al. 2006

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Chronic inflammation associated with tumor necrosis factor (TNF)-a and reactive oxygen species (ROS) is the hallmark of tuberculosis. Mycobacterium tuberculosis (MTB) directly stimulates human monocytes to secrete TNF-a. We show the augmented expression of TNF-a mRNA in MTB-infected monocytes by cellular activation and ROS was suppressed by allicin in a dose-dependent manner. Also, allicin enhanced the glutathione peroxidase activity, which correlated inversely with the downregulation of ROS and TNF-a in MTB-infected monocytes. Hence, allicin may prove to be a valuable natural antioxidant in combating tuberculosis.

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“…To monitor the genotoxiciry of environmental contaminants, some scientists proposed that when using endemic organisms such as bullheads and carp as sentinels, SCG should be useful because of its extreme sensitivity ~). If the bullheads and carp are suitable to detect pollutants in water, other [32] very valuable for monitoring genotoxic compounds [30] in the terrestrial ecosystem positive [33] Genotoxicity depends largely on the pH [34] positive: more hepatocyte damaged than lymphocytes [25] [41 [35] [36]…”

Section: The Methodsmentioning

confidence: 99%

Single-cell gel electrophoresis (SCG)-A review and discussion

Takeshita

Morimoto

1997

Environ Health Prev Med

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Single-cell gel electrophoresis (SCG) is a simple, sensitive and effective technique. Being able to reflect quantitatively the genotoxicity of many hazardous agents, it is promising for application in environmental genotoxic monitoring and the study of carcinogenesis. In clinics, it can be used to evaluate the DNA repair ability and monitor DNA breaks during cancer therapy. As a biomarker, it has its own merits and limitations, being different from other biomarkers such as sister chromatid exchange (SCE) test and micronudei (MN) assay. In many studies, it is more sensitive than SCE or MN.Combination studies with other biomarkers like SCE, MN, chromosomal aberration, bcl-2 and genetic polymorphisms have begun to demonstrate its great importance for the understanding of carcinogenesis and the genotoxicities of environmental factors.

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Assessment of genotoxic effects by Lindane

Cited by 148 publications

References 16 publications

Resveratrol–Cu(II) induced DNA breakage in human peripheral lymphocytes: Implications for anticancer properties

Resveratrol–Cu(II) induced DNA breakage in human peripheral lymphocytes: Implications for anticancer properties

Suppression of Mycobacterium tuberculosis induced reactive oxygen species (ROS) and TNF‐α mRNA expression in human monocytes by allicin

Single-cell gel electrophoresis (SCG)-A review and discussion

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