Assessment of Glucose Oxidase Based Enzymatic Fuel Cells Integrated With Newly Developed Chitosan Membranes by Electrochemical Impedance Spectroscopy

Bahar, Tahsin; Yazici, M. Süha

doi:10.1002/elan.201900743

Cited by 6 publications

(6 citation statements)

References 50 publications

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“…The most challenging aspect of this technique is interpreting the results displayed on the EIS spectrum, also known as the Nyquist plot. According to the existing literature, the total polarization resistance can be calculated by locating the intersection of the Nyquist and the x-axis during the anodic reaction [107,125].…”

Section: Anodic Reactionmentioning

confidence: 99%

Sustainably Sourced Mesoporous Carbon Molecular Sieves as Immobilization Matrices for Enzymatic Biofuel Cell Applications

Torrigino,

Nagel,

Peng

et al. 2023

Catalysts

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Ordered mesoporous carbon CMK-3 sieves with a hexagonal structure and uniform pore size have recently emerged as promising materials for applications as adsorbents and electrodes. In this study, using sucrose as the sustainable carbon source and SBA-15 as a template, CMK-3 sieves are synthesized to form bioelectrocatalytic immobilization matrices for enzymatic biofuel cell (EFC) electrodes. Their electrochemical performance, capacitive features, and the stability of enzyme immobilization are analyzed and compared to commercially available multi-walled carbon nanotubes (MWCNT) using cyclic voltammetry and electrochemical impedance spectroscopy (EIS). The anodic reaction in the presence of glucose oxidase (GOx) and ferrocene methanol (FcMeOH) on the sustainably sourced CMK-3-based electrodes produces bioelectrocatalytic current responses at 0.5 V vs. saturated calomel electrode (SCE) that are twice as high as on the MWCNT-based electrodes under saturated glucose conditions. For the cathodic reaction, the MWCNT-based cathode performs marginally better than the CMK-3-based electrodes in the presence of bilirubin oxidase (BOD) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS2−). The CMK-3-based EFCs assembled from the GOx anode and BOD cathode results in a power output of 93 μW cm−2. In contrast, the output power of MWCNT-based EFCs is approximately 53 μW cm−2. The efficiency of CMK-3 as a support material for biofuel cell applications is effectively demonstrated.

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Section: Anodic Reactionmentioning

confidence: 99%

Sustainably Sourced Mesoporous Carbon Molecular Sieves as Immobilization Matrices for Enzymatic Biofuel Cell Applications

Torrigino,

Nagel,

Peng

et al. 2023

Catalysts

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show abstract

“…Many materials were reported as potential candidates for the development of biomembranes such as cellulose [ 13 , 14 ], polycarbonate [ 15 ], hyaluronic acid [ 16 ], collagen [ 17 , 18 ] and chitosan [ 19 , 20 ]. However, chitosan-based materials are of particular interest due to their particular structural and functional characteristics, i.e., biocompatibility, high permeability, antibacterial and antimicrobial properties, adsorptive characteristics, and low cost [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Investigation of GOx Stability in a Chitosan Matrix: Applications for Enzymatic Electrodes

Chmayssem

Shalayel

Marinesco

et al. 2023

Sensors

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In this study, we designed a new biosensing membrane for the development of an electrochemical glucose biosensor. To proceed, we used a chitosan-based hydrogel that entraps glucose oxidase enzyme (GOx), and we crosslinked the whole matrix using glutaraldehyde, which is known for its quick and reactive crosslinking behavior. Then, the stability of the designed biosensors was investigated over time, according to different storage conditions (in PBS solution at temperatures of 4 °C and 37 °C and in the presence or absence of glucose). In some specific conditions, we found that our biosensor is capable of maintaining its stability for more than six months of storage. We also included catalase to protect the biosensing membranes from the enzymatic reaction by-products (e.g., hydrogen peroxide). This design protects the biocatalytic activity of GOx and enhances the lifetime of the biosensor.

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“…Many materials were reported as potential candidates for the development of (bio)membranes such as cellulose [13,14], polycarbonate [15], hyaluronic acid [16], collagen [17,18] and chitosan [19,20]. However, chitosan-based materials are of particular interest due to their particular structural and functional characteristics, i.e., biocompatibility, high permeability, antibacterial and antimicrobial properties, adsorptive characteristics and low cost [21].…”

Section: Introductionmentioning

confidence: 99%

Investigation of GOx Stability in Chitosan Matrix: Application for Enzymatic Electrodes

Chmayssem¹,

Shalayel²,

Marinesco³

et al. 2022

Preprint

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In this study, we designed a new biosensing membrane for the development of an electrochemical glucose biosensor. To proceed, we used a chitosan-based hydrogel that entraps glucose oxidase enzyme (GOx) and we crosslinked the whole matrix using glutaraldehyde, which is known for its quick and reactive crosslinking behavior. Then, the stability of the designed biosensors was investigated over time according to different storage conditions (in PBS solution at temperatures of 4 °C and 37 °C and in the presence or absence of glucose). In some specific conditions, we found that our biosensor is capable to keep its stability for more than six months of storage. We also included catalase to protect the biosensing membranes from the enzymatic reaction by-products (e.g., hydrogen peroxide). This design protects the biocatalytic activity of GOx and enhances the biosensor lifetime.

show abstract

Assessment of Glucose Oxidase Based Enzymatic Fuel Cells Integrated With Newly Developed Chitosan Membranes by Electrochemical Impedance Spectroscopy

Cited by 6 publications

References 50 publications

Sustainably Sourced Mesoporous Carbon Molecular Sieves as Immobilization Matrices for Enzymatic Biofuel Cell Applications

Sustainably Sourced Mesoporous Carbon Molecular Sieves as Immobilization Matrices for Enzymatic Biofuel Cell Applications

Investigation of GOx Stability in a Chitosan Matrix: Applications for Enzymatic Electrodes

Investigation of GOx Stability in Chitosan Matrix: Application for Enzymatic Electrodes

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