Assessment of glutathione-S-transferase and glutathione reductase in patients with squamous-cell carcinoma of buccal mucosa

Rawal, Rakesh; Patel, Devendra D.; Patel, Beena P.; Patel, Mona; Wadhwa, Manish; Patel, Prabhudas S; Jm, Bhatavdekar

doi:10.1002/(sici)1097-0215(19991210)83:6<727::aid-ijc5>3.0.co;2-p

Cited by 12 publications

(8 citation statements)

References 13 publications

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“…Our results also show greater staining intensity of GST Alpha, Mu, and Pi in more poorly differentiated relative to well-differentiated SCC tumors. Our pilot study data compare favorably to findings reported by Rawal et al who observed that the more poorly differentiated SCC tumors possessed higher GST activities (Rawal et al 1999). Several studies have provided mechanistic insights into how increased GST activities could provide a survival advantage to human cancers (Paumi et al 2001; Goto et al 2001).…”

Section: Discussionsupporting

confidence: 90%

“…Saroja et al demonstrated that relative to histologically normal mucosa, tissue derived from Stage IV oral SCC tissues demonstrate significantly higher levels of total GST activities (Saroja et al 1999). Similarly, Rawal et al determined that relative to corresponding adjacent histologically normal tissues, oral SCC samples possess significantly higher GST levels (Rawal et al 1999). Notably, neither of these previous reports determined GST expression in precancerous oral lesions.…”

Section: Introductionmentioning

confidence: 99%

“…Low GST levels could enhance the mutation-associated initiation phase of carcinogenesis, while the acquisition of increased GST function by oral SCC lesions could facilitate tumor cell survival during cancer progression. This premise is supported by the fact that highly progressed tumors frequently overexpress cytoprotective enzymes, including GSTs, which partially account for some of their resistance to various chemotherapeutic agents (Saroja et al 1999; Rawal et al 1999). …”

Section: Introductionmentioning

confidence: 99%

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Functional Activities and Immunohistochemical Cellular Distribution of Glutathione S-Transferases in Normal, Dysplastic, and Squamous Cell Carcinoma Human Oral Tissues

Giannini

Morse

Weghorst

et al. 2008

Clinical medicine. Oncology

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Clinical data show a strong correlation between tobacco and alcohol use and the development of oral squamous cell carcinoma (SCC). While this association implies that the oral mucosa actively metabolizes carcinogens, there is little information which depicts the carcinogen metabolizing enzymes within the oral cavity. Glutathione S-transferases (GSTs) primary function is to detoxify carcinogens by increasing their water solubility, GSTs represent key carcinogen metabolizing enzymes. Notably, individuals with a null phenotype for certain GST isoforms are at an increased risk to develop cancer. This study investigated the function and distribution of GSTs in human oral tissues. Our results from this pilot study showed a trend towards higher GST activities in SCC tissues relative to normal mucosa. Also, relative to normal tissues, the SCC and epithelial dysplasia samples showed a more intense and uniform GST intracellular distribution. GST activities are increased in many high grade cancers. Similarly, our data suggest that GST upregulation occurs in at least a subset of precancerous and malignant oral lesions.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Functional Activities and Immunohistochemical Cellular Distribution of Glutathione S-Transferases in Normal, Dysplastic, and Squamous Cell Carcinoma Human Oral Tissues

Giannini

Morse

Weghorst

et al. 2008

Clinical medicine. Oncology

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“…It has been shown that, oxidative stress is a major mediator of senescence and changes to intracellular GSH/GSSG status are critical for senescence [20]. Earlier reports from our laboratory, have documented significantly altered activities of detoxification enzymes in malignant tissues as compared to adjacent normal tissues of oral cancer patients [10,21]. These alterations in tissue enzyme levels may be a reflection of the imbalances in the detoxification pathway genes leading to altered glutathione pool.…”

Section: Discussionmentioning

confidence: 89%

Telomere attrition and telomerase activity are associated with GSTM1 polymorphism in oral cancer

Sainger

Shah

Telang

et al. 2009

CBM

Self Cite

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Telomere attrition is an important event during tumorigenesis regulated by factors including oxidative stress, mitochondrial function, DNA adducts etc. Critically short telomeres act as signal for telomerase activity in the cancer cells. To determine whether null genotype of GSTM1 gene has any association with telomere length shortening and telomerase activity, we analyzed telomere length, telomerase activity and GSTM1 polymorphism in oral tissues. We observed that malignant tissues exhibited shorter telomere length. Telomerase activity was observed in about 75% malignant tissues. 40% of the oral cancer patients exhibited GSTM1 polymorphism. Further, shorter telomere lengths were observed in patients having GSTM1 polymorphism. Also, the GSTM1 genotype showed negative correlation with telomerase activity and telomere length. Our study proposes role of GSTM1 polymorphism in telomere attrition and subsequent telomerase activity in the cancer cells. The results are suggestive of possible link between absence of GSTM1 gene and telomere length alterations.

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“…Particularly, GST-P has been recognized to be one of the best tumor markers in hepatocarcinogenesis and in oral carcinogenesis from rodents (Zhang et al 1992;Li et al 1997). Furthermore, it has been proposed that GST-P might be a useful marker for the detection of pre-neoplastic and neoplastic cells, in which an increased GST-P expression has been depicted in a wide variety of human tumors (Rawal et al 1999;Ribrag et al 2003). Our own research group has used this biomarker with success in chemoprevention trials (Agner et al 2004;Barbisan et al 2003).…”

Section: Resultsmentioning

confidence: 99%

Expression of placental glutathione S-transferase in rat tongue mucosa exposed to cigarette smoke

Ribeiro

Assis²

2007

J Mol Hist

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Glutatione S-transferases (GSTs) are a family of enzymes involved in detoxification of xenobiotics. Placental GST, known as GST-P, has been detected in tissues following exposure to carcinogenic agents being regarded a reliable biomarker of exposure and susceptibility in early phases of carcinogenesis. The aim of this study was to investigate the expressivity of GST-P positive foci in the rat tongue mucosa exposed to cigarette smoke by means of immunohistochemistry. A total of twelve male Wistar rats were distributed into two groups: negative control and experimental group exposed to cigarette smoke during 75 days. After experimental period, no histopathological changes in the tongue mucosa were evidenced in the negative control and the experimental group. However, a total of five GST-P positive foci were detected in two out of six animals exposed to cigarrette smoke. None control animals were noticed GST-P positive foci. These data indicate that expression of GST-P may reflect the carcinogenic effect of cigarette smoke as well as the genetic susceptibility of animals in relation to continuous carcinogens exposure.

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Assessment of glutathione-S-transferase and glutathione reductase in patients with squamous-cell carcinoma of buccal mucosa

Cited by 12 publications

References 13 publications

Functional Activities and Immunohistochemical Cellular Distribution of Glutathione S-Transferases in Normal, Dysplastic, and Squamous Cell Carcinoma Human Oral Tissues

Functional Activities and Immunohistochemical Cellular Distribution of Glutathione S-Transferases in Normal, Dysplastic, and Squamous Cell Carcinoma Human Oral Tissues

Telomere attrition and telomerase activity are associated with GSTM1 polymorphism in oral cancer

Expression of placental glutathione S-transferase in rat tongue mucosa exposed to cigarette smoke

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