Assessment of glycerol usage by five different purple non-sulfur bacterial strains for bioplastic production

Padovani, Giulia; Emiliani, Giovanni; Giovanelli, Alessio; Traversi, Maria Laura; Carlozzi, Pietro

doi:10.1016/j.jece.2017.12.050

Cited by 23 publications

(12 citation statements)

References 28 publications

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“…The cell dry weight (CDW) concentration, carbohydrate and protein content of the biomass, elemental analysis of the biomass (C, H, N, O) and the heat of combustion of ash-free biomass (H B ) were determined in accordance with Carlozzi and Sacchi (2001). The content of PHB in the biomass was determined in accordance with Padovani et al (2018). The bacteriochlorophyll (Bchl) was measured according to Carlozzi et al (2006) and the amount of TVFAs was determined at 530 nm, in accordance with Mato et al (2005).…”

Section: Methodsmentioning

confidence: 99%

Hydroxytyrosol rich-mixture from olive mill wastewater and production of green products by feeding Rhodopseudomonas sp. S16-FVPT5 with the residual effluent

Carlozzi

Seggiani

Capperucci

et al. 2019

Journal of Biotechnology

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This study disserts on the exploitation of olive mill wastewater (OMW) for the production of both bio-based polyβ-hydroxybutyrate (PHB) and hydrogen (H 2) by using the residual effluent as feedstock for growing purple bacteria after the recovery of hydroxytyrosol-rich mixtures. In particular, Rhodopseudomonas sp. S16-FVPT5 was fed with either the virgin OMW or dephenolized-OMW (d-OMW). For polyphenols removal, the OMW was treated with activated carbon; subsequently, acidified ethanol (pH = 3.1) at 50°C was used as extractor solvent for obtaining hydroxytyrosol-rich mixtures. The maximum hydroxytyrosol content in the resultant polyphenolic mixture was 2.02 g/L. The highest co-production of PHB (315 mg PHB/L) and H 2 (2236 mL H 2 /L) were achieved feeding Rhodopseudomonas sp. S16-FVPT5 with pure d-OMW. The highest hydrogen yield (4.55 L(H 2)/L d-OMW) was obtained feeding the bacterium with d-OMW, diluted at 25%; by increasing the content of d-OMW into the culture broth the hydrogen yield progressively decreased. Lower results were obtained by feeding the bacterium with a synthetic medium, the cumulative hydrogen was 1855 mL H 2 /L); the PHB was 101 mg PHB/L. The highest theoretical light conversion efficiency was 2.36% with the synthetic medium and 1.99% when feeding Rhodopseudomonas sp. S16-FVPT5 with d-OMW diluted with water 50%, v/v.

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Section: Methodsmentioning

confidence: 99%

Hydroxytyrosol rich-mixture from olive mill wastewater and production of green products by feeding Rhodopseudomonas sp. S16-FVPT5 with the residual effluent

Carlozzi

Seggiani

Capperucci

et al. 2019

Journal of Biotechnology

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“…Cell dry-weight concentration (CDWC) and bacteriochlorophyll (Bchl) concentration were determined in accordance with Carlozzi and Sacchi [16]. The PHB content accumulated in the bacterial cells was measured by HPLC (High-Performance Liquid Chromatography) using the Thermo Finnigan-Spectra System 6000 LP (Thermofinnigan, San José, CA, USA), following the procedure reported elsewhere [15]. The organic acid concentration in the bacteria cultures was determined as reported elsewhere [19] by using the HPLC (Thermo Finnigan-Spectra System 6000 LP) with a C18 analytical column (250 × 4.6 mm) operating at 25 • C. The cells were separated from the culture suspension by disposable syringe filter units (MFS-13 mm, pore size 0.45 µm) and the supernatant sample was injected into the C18 analytical column for the analysis of the lactic acid concentration.…”

Section: Methodsmentioning

confidence: 99%

Photofermentative Poly-3-Hydroxybutyrate Production by Rhodopseudomonas sp. S16-VOGS3 in a Novel Outdoor 70-L Photobioreactor

et al. 2018

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In the present study, the performance of a 70 L photobioreactor, operating outdoors, was investigated using a purple bacterial strain as Rhodopseudomonas sp. S16-VOGS3 for producing poly-3-hydroxybutyrate (PHB). The novel photobioreactor was equipped with 5 rows L-shaped; the bottom of every row was placed in a stainless-steel tank containing water with controlled temperature. The photofermentation trials were carried out under fed-batch mode and under a semi-continuous regimen using lactic acid as the carbon source. The effect of the irradiance and the carbon/nitrogen ratio on the PHB accumulation was investigated, in order to evaluate the optimal bacterial growth. The results showed the feasibility of the prototype photobioreactor for the production of PHB by Rhodopseudomonas sp. S16-VOGS3 under the natural light/dark cycle. During the fed-batch growth (144 h long), the cumulative PHB increased quickly reaching a maximum value of 377 mg/L and decreased to 255 mg/L during the semi-continuous regimen (336 h long).

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“…CDWC and bacteriochlorophyll (Bchl) concentration were determined in accordance with Carlozzi and Sacchi [15]. The PHB content accumulated in the bacterial cells was measured by High-Performance Liquid Chromatography (HPLC) Thermo Finnigan-Spectra System 6000 LP, following the method reported elsewhere [14]. About 6 mg of lyophilized biomass was weighed and utilized for acid digestion to crotonic acid by boiling the biomass in 1 mL of pure sulfuric acid in screw-cap glass test tubes for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, the same bacterial strain used in the present study (Rhodopseudomonas sp. S16-VOGS3) was identified as a potential candidate for employing crude glycerol coming from biodiesel industry [14]. The authors obtained (indoors) a PHB-rich biomass with a content of 18% on dry-weight biomass, demonstrating that a degradable plastic material as PHB could be efficiently produced by feeding Rhodopseudomonas sp.…”

Section: Photobioreactor Performancementioning

confidence: 99%

“…In this study, a wild strain of Rhodopseudomonas sp. S16-VOGS3, previously selected as PHBproducer [14], was used for producing PHB in a novel outdoor L-shaped row photobioreactor (L-SRP), under the natural light/dark cycle. The photo-fermentation trial was carried out initially in batch mode and then semi-continuous mode, using lactic acid as carbon source.…”

Section: Introductionmentioning

confidence: 99%

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Photofermentative Poly-3-Hydroxybutyrate Production by <em>Rhodopseudomonas</em> Sp. S16-VOGS3 in a Novel Outdoor L-Shaped Row Photobioreactor

Carlozzi¹,

Seggiani²,

Cinelli³

et al. 2018

Preprint

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In the present study, the performance of a 70 L photobioreactor, operating outdoors, was investigated using a purple bacterial strain as Rhodopseudomonas sp. S16-VOGS3 for producing poly-3-hydroxybutyrate (PHB). The novel photobioreactor was equipped with 5L-shaped rows; the bottom of every row was placed in a stainless-steel tank containing water with controlled temperature. The photofermentation trials were carried out under fed-batch mode and under a semi-continuous regimen using lactic acid as carbon source. The effect of the irradiance and the C/N ratio on the PHB accumulation was investigated, in order to evaluate the optimal bacterial growth. The results showed the feasibility of the prototype photobioreactor for the production of PHB by Rhodopseudomonas sp. S16-VOGS3 under the natural light/dark cycle. During the fed-batch growth (144 h long), the cumulative PHB increased quickly reaching the maximum value of 377 mg/L and decreased to 255 mg/L during the semi-continuous regimen (336 h long).

show abstract

Assessment of glycerol usage by five different purple non-sulfur bacterial strains for bioplastic production

Cited by 23 publications

References 28 publications

Hydroxytyrosol rich-mixture from olive mill wastewater and production of green products by feeding Rhodopseudomonas sp. S16-FVPT5 with the residual effluent

Hydroxytyrosol rich-mixture from olive mill wastewater and production of green products by feeding Rhodopseudomonas sp. S16-FVPT5 with the residual effluent

Photofermentative Poly-3-Hydroxybutyrate Production by Rhodopseudomonas sp. S16-VOGS3 in a Novel Outdoor 70-L Photobioreactor

Photofermentative Poly-3-Hydroxybutyrate Production by <em>Rhodopseudomonas</em> Sp. S16-VOGS3 in a Novel Outdoor L-Shaped Row Photobioreactor

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