Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models

Rhee, Jihye; Han, Eun‐Jung; Nam, Kuk Jin; Lim, Kang Hyeon; Rah, Yoon Chan; Park, Saemi; Koun, Soonil; Park, Hae-Chul; Choi, June

doi:10.1016/j.ijporl.2019.109611

Cited by 7 publications

(7 citation statements)

References 52 publications

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“…Given that zebrafish are morphologically similar to hair cells in the human inner ear, zebrafish are widely used to assess ototoxicity. In their most recent study, Rhee et al [ 17 ] used the zebrafish model to assess damage and developmental toxicity of hair cells caused by PM 2.5 exposure. They observed significant hair cell damage after exposure to PM 2.5 , which was dose-dependent and more severe after prolonged exposure.…”

Section: Discussionmentioning

confidence: 99%

“…In 2002, Ritz et al [7] first discovered the association between ambient air pollution and congenital malformations, which has since been confirmed by numerous researchers [8][9][10][11][12][13]. Although evidence from in vivo and in vitro studies [14][15][16][17][18] seem to indicate that air pollution exposure during pregnancy causes congenital ear malformations, evidence from epidemiological studies remain limited.…”

Section: Introductionmentioning

confidence: 99%

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Association Between Maternal Exposure to SO2 and Congenital Ear Malformations in Offspring: A Population-Based Case-Control Study in Liaoning Province, China

Li²,

Gong³

et al. 2022

Int J Public Health

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Objectives: To examine associations between maternal sulfur dioxide (SO2) exposure and congenital ear malformations risk in offspring.Methods: We surveyed 1676 cases with congenital ear malformations and 7950 controls from the Maternal and Child Health Certificate Registry of Liaoning Province between 2010 and 2015. SO2 concentrations were obtained from the Municipal Environment Protection Bureau of Liaoning Province. Multivariable logistic regression models and Restricted cubic splines (RCS) model were used to assess the aforementioned association.Results: There were significant associations between maternal SO2 exposure and congenital ear malformations risk during the 3 months before conception (OR Q4 vs. Q1 = 1.93, 95% CI = 1.43–2.59) and the 3 months after conception (OR Q4 vs. Q1 = 1.63, 95% CI = 1.22–2.18). Similar results were obtained in the analysis of single-month exposure windows, except for the third month before conception and the third month after conception. Moreover, these findings were broadly consistent across subgroups and robust in sensitivity analyses. There were non-linear dose-response associations between SO2 exposure and congenital ear malformations based on restricted cubic spline model analysis.Conclusion: Maternal SO2 exposure is associated with increased congenital ear malformations risk in offspring.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Association Between Maternal Exposure to SO2 and Congenital Ear Malformations in Offspring: A Population-Based Case-Control Study in Liaoning Province, China

Li²,

Gong³

et al. 2022

Int J Public Health

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“…The average number of hair cells in two main regions, the anterior lateral line (ALL) system, including the supraorbital [SO1 and SO2], otic [O1], and occipital [OC1] nerves, and the posterior lateral line (PLL) system, including the neuromasts of the trunk and tail of zebrafishes (p 3–6), was determined using a fluorescence microscope at 7 dpf (LSM5 PASCAL; Carl Zeiss, Germany). 9,17,18,25,27 The hair cells were identified by our two experts, and they were evaluated in all experimental and control conditions (n = 29–40 for each of the concentrations)…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Zebrafish (Danio rerio) has several advantages for assessing developmental toxicity owing to its high fecundity rate and a well characterized developmental morphological scoring system. [13][14][15][16][17][18][19] Zebrafish is known to be a good model to study the development and teratogenicity of organ systems and structures due to its inexpensive, rapid development, and transparency. 14,16,19 The hair cells in the inner ear of humans are important organs that recognize sound from the external environment and perceive the sense of rotation and movement.…”

Section: Introductionmentioning

confidence: 99%

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In vivo assessment of the toxicity of electronic cigarettes to zebrafish (Danio rerio) embryos, following gestational exposure, in terms of mortality, developmental toxicity, and hair cell damage: Toxicity of E-cigs to zebrafish embryos

et al. 2020

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With the ban of conventional cigarettes from public spaces, electronic cigarette (E-cig) liquids have emerged as a nicotine replacement treatment for smoking cessation. However, consumers possess little knowledge of the ingredients and health effects of E-cig liquids following exposure. This study evaluated hair cell damage and developmental toxicities following gestational exposure to E-cig liquids. Zebrafish embryos were exposed to E-cig liquids at different concentrations (0.1%, 0.2%, and 0.4%). Embryonic developmental toxicity and hair cell damage was evaluated at 6 and 7 d, respectively, after fertilization. The average number of hair cells in the anterior lateral line (ALL) and posterior lateral line (PLL) following E-cig exposure was compared to that of the control. Morphological abnormalities and heart rate were evaluated. E-cig liquids significantly damaged the hair cells in the ALL, compared to the control (control; 52.85 ± 5.29 cells, 0.1% E-cig; 49.43 ± 7.70 cells, 0.2% E-cig; 40.68 ± 12.00 cells, 0.4% E-cig; 32.14 ± 20.75%; n = 29–40; p < 0.01). At high concentrations, E-cig liquids significantly damaged the hair cells in the PLL (control; 36.88 ± 5.43 cells, 0.1% E-cig; 33.06 ± 5.21 cells, 0.2% E-cig; 30.95 ± 8.03 cells, 0.4% E-cig; 23.72 ± 15.53%, n = 29–40; p < 0.01). No morphological abnormalities in body shape, somites, notochord, tail, and pectoral fin were observed; however, abnormalities were observed in the dorsal fin and heart rate at high concentrations. Thus, gestational exposure to E-cigs significantly damaged hair cells in a concentration-dependent manner and induced developmental toxicities to the dorsal fin and heart rate at high concentrations.

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Maternal exposure to fine particulate matter before and during pregnancy, and the risk of birth defects: A population-based study

Zhen,

Li,

et al. 2025

Ecotoxicology and Environmental Safety

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Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models

Cited by 7 publications

References 52 publications

Association Between Maternal Exposure to SO2 and Congenital Ear Malformations in Offspring: A Population-Based Case-Control Study in Liaoning Province, China

Association Between Maternal Exposure to SO2 and Congenital Ear Malformations in Offspring: A Population-Based Case-Control Study in Liaoning Province, China

In vivo assessment of the toxicity of electronic cigarettes to zebrafish (Danio rerio) embryos, following gestational exposure, in terms of mortality, developmental toxicity, and hair cell damage: Toxicity of E-cigs to zebrafish embryos

Maternal exposure to fine particulate matter before and during pregnancy, and the risk of birth defects: A population-based study

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Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models

Cited by 7 publications

References 52 publications

Association Between Maternal Exposure to SO2 and Congenital Ear Malformations in Offspring: A Population-Based Case-Control Study in Liaoning Province, China

Association Between Maternal Exposure to SO2 and Congenital Ear Malformations in Offspring: A Population-Based Case-Control Study in Liaoning Province, China

In vivo assessment of the toxicity of electronic cigarettes to zebrafish (Danio rerio) embryos, following gestational exposure, in terms of mortality, developmental toxicity, and hair cell damage: Toxicity of E-cigs to zebrafish embryos

Maternal exposure to fine particulate matter before and during pregnancy, and the risk of birth defects: A population-based study

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Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models