“…People living in the Akaki catchment are using river water for irrigation, livestock watering, washing, cooking and other domestic needs [23], which may expose them to toxic heavy metals in the rivers. This is of concern, since various epidemiological studies have reported that exposure to toxic heavy metals is associated with health disorders and diseases including cardiovascular, gastrointestinal, nervous system, and kidney disease [12,15,[31][32][33][34][35][36][37][38]. Excess iron in the body leads to free radical formation through Fenton reaction, which causes adverse health effects including hemochromatosis, liver cirrhosis and siderosis [39].…”