Assessment of Heterosis Based on Parental Genetic Distance Estimated With SSR and SNP Markers in Upland Cotton (Gossypium Hirsutum L.)

Abstract

Background: Heterosis has been extensively utilized in different crops and made a significant contribution to global food security. Genetic distance (GD) is one of the valuable criteria for selecting parents in hybrid breeding. The objectives of this study were to estimate the GD between parents using both simple sequence repeat (SSR) markers and single nucleotide polymorphism (SNP) markers and to investigate the efficiency of the prediction of hybrid performance based on GD. The experiment comprised of four male parents, 282 female parents and 1128 F1, derived from NCII mating scheme. The hybrids, their parents with two check cultivars were evaluated for two years. Performance of F1, mid-parent heterosis (MPH), and best parent heterosis (BPH) were evaluated for ten agronomic and fiber quality traits. Results: Heterosis was observed in all hybrids and, the traits like plant height, boll number, boll weight and lint percentage exhibited higher heterosis than the fiber quality traits. Correlations were significant between parental and F1 performances. The F­1 performances between three hybrid sets (Elite×Elite, Exotic×Elite, and Historic×Elite) showed significant differences in eight traits. The correlation of the GD assessed by both SSR and SNP markers was significantly positive. The cluster analysis based on GD results estimated using SNP showed that all the female parents divided into five groups and the F1 performance between these five groups showed significant differences in seven traits. The correlation between GD and F1 performance, MPH and BPH were significant for lint percentage and micronaire. Conclusions: Our results suggested that GD between parents could be helpful in heterosis prediction for certain traits. This study reveals that molecular marker analysis can serve as a basis for assigning germplasm into heterotic groups and to provide guidelines for parental selection in hybrid cotton breeding.

In-Depth Genetic Diversity and Population Structure of Endangered Peruvian Amazon Rosewood Germplasm Using Genotyping by Sequencing (GBS) Technology

“…Genetic distance is a degree of genomic differences between species or populations and it is calculated by some numerical method [38][39][40][41][42][43][44][45][46][47][48]. Very recent studies confirmed genetic distance as a valuable criterion for the selection of parents that can be used in breeding activities [49,50]. Germplasm resources proposing the highest level of genetic distance must be properly conserved and utilize in future breeding programs for their improvement [29].…”