Assessment of Human Sperm Morphology by Strict Criteria: Comparison of Wet Preparation Versus Stained With the Modified Diff-Quik Method

Oral, Engin; Yetis, O; Elibol, Feyhan; Şenol, Hülya; İrez, Tülay; Aksu, Feridun

doi:10.1080/01485010290031628

Cited by 14 publications

(7 citation statements)

References 16 publications

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“…The effect of different staining techniques on sperm morphology and morphometry has been investigated (Davis and Gravance, 1993;Lacquet et al, 1996;Oral et al, 2002). A few studies acknowledge the fact that some staining techniques either increased or decreased the sperm head dimensions (Katz et al, 1986;Meschede et al, 1993;Franken et al, 2000;Hidalgo et al, 2006), but that in the case of The SCA w system recognizes the acrosome (yellow), head (blue) and midpiece (green).…”

Section: Discussionmentioning

confidence: 99%

Morphometric dimensions of the human sperm head depend on the staining method used

et al. 2010

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Different staining techniques change the morphometric dimensions of the human sperm head, probably due to the fact that either the fixatives or stains are not iso-osmotic in relation to human semen. Since these changes in sperm head dimensions are not uniform, care should be taken when selecting a staining technique. Ideally, stained spermatozoa should have dimensions as close to spermatozoa in fresh semen as possible, as was found with the SB staining method, resulting in accurate evaluations of sperm head morphometry.

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Section: Discussionmentioning

confidence: 99%

Morphometric dimensions of the human sperm head depend on the staining method used

et al. 2010

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“…The first step is evaluation of motility, the number of sperm and the proportions of live and dead sperm (Guzick et al 2001, Hidalgo et al 2006. The next step is morphological evaluation, which is increasingly expanded to include detailed morphometry of the spermatozoon (Oral et al 2002). There are situations, however, in which a motile and morphologically normal spermatozoon is incapable of fertilisation, e.g.…”

Section: Introductionmentioning

confidence: 99%

Comparison of different chromatin staining techniques for bull sperm

Andraszek¹,

Banaszewska

Czubaszek³

et al. 2014

Arch. Anim. Breed.

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Morphological analysis of semen is a very important step in fertility assessment, but many semen defects are not detectable at the morphological level. These include pathological changes in sperm chromatin structure. During mammalian spermiogenesis, histone proteins associated with DNA structure are replaced by specific protamines, with which chromatin does not form nucleosomal complexes. In the fully developed, mature sperm, the histones are replaced with protamines. Disruptions of nucleoprotein structure can be treated as possible indicators of the biological value of spermatozoa. The experimental material consisted of sperm from one-and-a-half-year-old bulls, isolated post mortem from the tail of the epididymis. The smears were stained with silver nitrate (AgNO 3 ), acridine orange (AO), aniline blue (AB) and chromomycin A3 (CMA3). Sperm dimensions largely depend on individual variability among the bulls. In most cases, differences in sperm dimensions were identified between individuals, which was confirmed in the statistics. Sperm with elevated, abnormal histone levels were proportionally quite scarce (1.4 %). Studies of nuclear proteins in the context of infertility demonstrate the important influence of normal chromatin structure on sperm functions.

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“…The standard semen analysis is the crucial tool in diagnosis and treatment of human and animal infertility (22). The most frequently assessed, and the most important semen parameters include concentration, motility, and morphology of the sperm (13).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the capacity of these cells to move is crucial at each moment of fertilisation since it indirectly facilitates the passage of the spermatozoon through the zona pellucida of the oocyte (6). Of the three parameters, sperm morphology is the most objective index of in vivo and in vitro fertility (22). Abnormal morphological structure of spermatozoa can be indicative of pathology underlying spermatogenesis disorders.…”

Section: Discussionmentioning

confidence: 99%

Use of Silver Nitrate for the Assessment of Sperm Measurements in Selected Farm and Free-Living Animal Species

Andraszek

Banaszewska

Szostek

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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The study was conducted on spermatozoa of selected farm and free-living animal species, isolated post mortem from the tail of the epididymis, and stained with silver nitrate -AgNO3. The material was collected from pigs, goats, wild boar, and European roe deer. Twenty morphologically normal spermatozoa randomly selected from each animal and well visible under the microscope, were analysed. The following measurements were considered: head length, width, perimeter and area, acrosome area, mid-piece length, tail length, and overall sperm length. AgNO3 staining differentiated the acrosomal (light hue) and distal (dark hue) part of the sperm head, and a light-hued mid-piece was visible within the sperm tail. Silver nitrate staining revealed species and variety-related differences, particularly in reference to the sperm head. Clear-cut differentiation within the head and tail area made it possible to perform detailed morphometric measurements of the spermatozoa.

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Assessment of Human Sperm Morphology by Strict Criteria: Comparison of Wet Preparation Versus Stained With the Modified Diff-Quik Method

Cited by 14 publications

References 16 publications

Morphometric dimensions of the human sperm head depend on the staining method used

Morphometric dimensions of the human sperm head depend on the staining method used

Comparison of different chromatin staining techniques for bull sperm

Use of Silver Nitrate for the Assessment of Sperm Measurements in Selected Farm and Free-Living Animal Species

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