Assessment of <i>in vitro</i> cytotoxicity of imidazole ionic liquids and inclusion in targeted drug carriers containing violacein

Berti, Ignacio Rivero; Rodenak-Kladniew, Boris; Onaindia, Celeste; Adam, Claudia G.; Islan, Germán Abel; Castro, Guillermo R.

doi:10.1039/d0ra05101b

Cited by 19 publications

(6 citation statements)

References 43 publications

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“…While the concentrations of violacein showed the best antiviral activity at 1.25 µM against HSV-1 (i.e., strains KOS, VR-733, RV-SA11) with inhibition percentages in the range of 17.75%-24.27% and compared with acyclovir with inhibition percentages close to 100% Besides, the antiviral violacein concentration assayed was 35.5 to 8.9 times lower compared to the acyclovir (Andrighetti-Fröhner et al, 2003). In general, violacein cytotoxicity in nontumoral cell lines is in the range of 5 µM-10 μM, meanwhile tumoral cell lines are more sensitive to the biodye with a cytotoxic range of 0.71 µM to about 5 µM (de Sousa Leal et al, 2015;Rivero Berti et al, 2020). Several mechanisms of violacein cytotoxicity including disturbance of mammalian cell membrane and polarization of mitochondrial membrane, disruption of actin function, p44/42 mitogen activated protein kinase (MAPK), apoptosis mediated by caspase 3, etc.…”

Section: Violacein Cytotoxicitymentioning

confidence: 95%

“…Despite the different surface characteristics of the three tested viruses, all shared hydrophobic moieties on their surfaces that could interact with water-insoluble molecules, such as violacein. Since the interaction of violacein with molecules with hydrophobic motifs (i.e., non-ionic surfactants, cyclodextrins, aromatic ionic liquids, lipid carriers) were previously reported (de Azevedo et al, 2000;Rivero Berti et al, 2019;Rivero Berti et al, 2020;Rivero Berti et al, 2022). It is expected that hydrophobic interactions appear to be the unspecific major mechanism of interaction between the virus and violacein.…”

Section: Violacein Antiviral Activitiesmentioning

confidence: 99%

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Potential biocide roles of violacein

Berti

Gantner

Rodrı́guez

et al. 2023

Front. Nanotechnol.

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Violacein is a pigment produced by Gram-negative bacteria, which has shown several beneficial biological activities. The most relevant activities of violacein include the interference in the physiological activities of biological membranes, inhibition of cell proliferation, antioxidant, and anti-inflammatory activities. Moreover, the antiviral activities of violacein against some enveloped and non-enveloped viruses have also been reported. Violacein showed a wide spectrum of protease inhibition, both experimentally and in silico. Other in silico studies have suggested that violacein binds to the SARS-CoV-2 spike. Empirical physicochemical studies indicate that violacein (or, occasionally, its derivatives) may be administered orally to treat different disorders. In addition, different alternatives to product violacein, and molecular devices for delivery of this pigment are reviewed.

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Section: Violacein Cytotoxicitymentioning

confidence: 95%

Section: Violacein Antiviral Activitiesmentioning

confidence: 99%

Potential biocide roles of violacein

Berti

Gantner

Rodrı́guez

et al. 2023

Front. Nanotechnol.

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“…Thus, Viol could be a promising new drug but also an excellent therapeutic agent if a suitable drug delivery system is developed. Since Viol is a hydrophobic molecule, lipid systems can be considered as a potential best drug carrier ( Rivero Berti et al, 2020 ). Among them, liposomes, and lipid nanoparticles are probably the most popular structures employed for the development of drug delivery systems because of their simple preparation, non-toxic, easy scale-up, and reproducibility.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Active Release of Violacein Present in Nanostructured Lipid Carrier by Lipase Encapsulated in 3D-Bioprinted Chitosan-Hydroxypropyl Methylcellulose Matrix With Anticancer Activity

et al. 2022

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Violacein (Viol) is a bacterial purple water-insoluble pigment synthesized by Chromobacterium violaceum and other microorganisms that display many beneficial therapeutic properties including anticancer activity. Viol was produced, purified in our laboratory, and encapsulated in a nanostructured lipid carrier (NLC). The NLC is composed of the solid lipid myristyl myristate, an oily lipid mixture composed of capric and caprylic acids, and the surfactant poloxamer P188. Dormant lipase from Rhizomucor miehei was incorporated into the NLC-Viol to develop an active release system. The NLC particle size determined by dynamic light scattering brings around 150 nm particle size and ζ≈ −9.0 mV with or without lipase, but the incorporation of lipase increase the PdI from 0.241 to 0.319 (≈32%). For scaffold development, a 2.5 hydroxypropyl methylcellulose/chitosan ratio was obtained after optimization of a composite for extrusion in a 3D-bioprinter developed and constructed in our laboratory. Final Viol encapsulation efficiency in the printings was over 90%. Kinetic release of the biodye at pH = 7.4 from the mesh containing NLC-lipase showed roughly 20% Viol fast release than without the enzyme. However, both Viol kinetic releases displayed similar profiles at pH = 5.0, where the lipase is inactive. The kinetic release of Viol from the NLC-matrices was modeled and the best correlation was found with the Korsmeyer-Peppas model (R2 = 0.95) with n < 0.5 suggesting a Fickian release of Viol from the matrices. Scanning Electron Microscope (SEM) images of the NLC-meshes showed significant differences before and after Viol’s release. Also, the presence of lipase dramatically increased the gaps in the interchain mesh. XRD and Fourier Transform Infrared (FTIR) analyses of the NLC-meshes showed a decrease in the crystalline structure of the composites with the incorporation of the NLC, and the decrease of myristyl myristate in the mesh can be attributed to the lipase activity. TGA profiles of the NLC-meshes showed high thermal stability than the individual components. Cytotoxic studies in A549 and HCT-116 cancer cell lines revealed high anticancer activity of the matrix mediated by mucoadhesive chitosan, plus the biological synergistic activities of violacein and lipase.

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“…Violacein associated with lipid carriers has shown toxicity capable of controlling cancer cell growth (Rivero‐Berti et al . 2020).…”

Section: Introductionmentioning

confidence: 99%

Violacein and its antifungal activity: comments and potentialities

Castro

Portela

Fávaro

et al. 2022

Letters in Applied Microbiology

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Violacein is an important natural antimicrobial pigment that is mainly produced by Chromobacterium violaceum and Janthinobacterium lividum. It presents a significant range of effects against phytopathogenic and human fungi, besides being featured as having low toxicity, and by its important ecological role in protecting amphibian species and applications in dyed medical fabric. The hypothesis about violacein's action mechanisms against mucormycosis (Rhizopus arrhizus) and candidiasis (Candida auris) is herein discussed based on data available in the scientific literature.

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Assessment of in vitro cytotoxicity of imidazole ionic liquids and inclusion in targeted drug carriers containing violacein

Abstract: Violacein (Viol) is a pigment produced by several Gram-negative bacteria with many bioactivities, such as anticancer, virucide, and antiparasitic.

Cited by 19 publications

References 43 publications

Potential biocide roles of violacein

Potential biocide roles of violacein

Enzymatic Active Release of Violacein Present in Nanostructured Lipid Carrier by Lipase Encapsulated in 3D-Bioprinted Chitosan-Hydroxypropyl Methylcellulose Matrix With Anticancer Activity

Violacein and its antifungal activity: comments and potentialities

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