Assessment of Pradosia huberi effects on the reproductive system of male rats

Abstract

Pradosia huberi is a species found in the Amazon region and used as an antiulcerogenic and gastroprotective agent; however, phytochemical analysis has revealed the presence of compounds with potential toxic effects on the reproductive system. For the evaluation of the toxicity of P. huberi on male fertility, male Wistar rats were divided into four groups: one control (distilled water p.o.) and three treated (hydroalcoholic extract of the stem bark of P. Huberi (PH-HAE) at doses of 1.22, 6.1, and 30.5 mg/kg p.o.) once daily, for 63 days. In the last week of treatment (from the 57th to the 63rd day), the rats were mated with untreated virgin females (n ¼ 30/group) and were killed on day 64. To investigate the toxic potential of PH-HAE on the reproductive system of rats the following parameters were evaluated: sperm production, genotoxicity, and general development. The production of gametes and their morphology did not differ between control and treated groups. Treatment with PH-HAE did not result in fewer vaginal plugs formed, indicating that the ability to mate was not impaired, but caused an increase of 14.3 and 10.8% in the preimplantation loss index, a reduction of 14.3 and 10.8% in the implantation index, and a reduction of 5.6 and 8.2% in the postimplantation loss index of female rats mated with rats treated with 6.1 and 30.5 mg/kg, respectively, indicating a possible toxic action of PH-HAE on the reproductive system of rats.

Carnosine Remedial Effect on Fertility of Male Rats Receiving Cyclophosphamide, Hydroxydaunomycin, Oncovin and Prednisone (CHOP)

“…Decreased body weight or weight gain may reflect anorexia or systemic toxicity (Silva et al, 2016). The decreased testes weight may originate from defect in spermatogenesis, which results in germ cells loss (Vaisheva et al, 2007).…”

“…Our observation that CHOP decreased body weight and sex organs weight is in line with other reports (Kim et al., 1999; Vaisheva et al., 2007; Ward et al., 1988). Decreased body weight or weight gain may reflect anorexia or systemic toxicity (Silva et al., 2016). The decreased testes weight may originate from defect in spermatogenesis, which results in germ cells loss (Vaisheva et al., 2007).…”