Assessment of IL-1β and VEGF concentration in a rat model during orthodontic tooth movement and extracorporeal shock wave therapy

Hazan-Molina, Hagai; Reznick, Abraham Z.; Kaufman, Hanna; Aizenbud, Dror

doi:10.1016/j.archoralbio.2012.09.012

Cited by 25 publications

(25 citation statements)

References 45 publications

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“…This difference might be explained by the use of different cytokine detection methods: ELISA in the preliminary study and immunohistochemical staining in this study. The inability to detect cytokines other than IL‐1β in the gingival crevicular fluid in the preliminary study by ELISA and the contrast in the expression pattern between IL‐1β‐ and TRAP‐positive cells led us to search for a different and more sensitive detection method in a different medium. Immunohistochemical staining is a very sensitive and specific method for the detection of different proteins in tissue.…”

Section: Discussionmentioning

confidence: 85%

“…In a preliminary study , we observed a contrasting result regarding the IL‐1β concentration in the gingival crevicular fluid: it was statistically significantly higher on the second day in the Spring + ESWT group compared with the Spring group. Yet, the number of TRAP‐positive cells per area was reduced in the Spring + ESWT group compared with the Spring group, on the compressed side of the periodontal ligament, on day 3, as presented in this report.…”

Section: Discussionmentioning

confidence: 87%

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Periodontal cytokines profile under orthodontic force and extracorporeal shock wave stimuli in a rat model

Hazan-Molina

Reznick

Kaufman

et al. 2014

J of Periodontal Research

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 87%

Periodontal cytokines profile under orthodontic force and extracorporeal shock wave stimuli in a rat model

Hazan-Molina

Reznick

Kaufman

et al. 2014

J of Periodontal Research

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“…Then, injected AMG-9810 (10 nmol) in the parodontium as described above. The gingival crevicular fluid (GCF) was collected and detected IL-1β content follow by previously described [17]. IL-1β level was measured in duplicate using a commercial ELISA kit (R&D Systems, USA), and sample values were read from the standard curve set to 450 nm with a wavelength correction between 540 and 570 nm, and measurement units expressed as pg/ml.…”

Section: Elisa Assaymentioning

confidence: 99%

Blocking of TRPV-1 in the parodontium relieves orthodontic pain by inhibiting the expression of TRPV-1 in the trigeminal ganglion during experimental tooth movement in rats

Gao

Liu

Zhang

et al. 2016

Neuroscience Letters

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“…The groups were as follows: Glucose concentrations and body weight were measured once a week in all 60 rats. Five rats were randomly selected from each group for the collection of gingival crevicular fluid (GCF) from the mesio-buccal region of the maxillary right molar under anesthesia as previously described [22,23]. GCF was collected during the second, fourth and sixth weeks after induction of periodontitis.…”

Section: Production Of Experimental Rats With Dm and Pdmentioning

confidence: 99%

Study of TNF-α, IL-1βand LPS Levels in the Gingival Crevicular Fluid of a Rat Model of Diabetes Mellitus and Periodontitis

Zhu-lin

2013

Disease Markers

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OBJECTIVE: In this study, we sought to investigate the dynamic changes in the levels of TNF-α, IL-1β and LPS in the gingival crevicular fluid (GCF) in a rat model of diabetes mellitus (DM) and periodontitis (PD). Additionally, we evaluated alveolar bone loss and the histopathological response associated with experimental diabetes mellitus and experimental periodontitis.METHODS: DM and PD were induced together in 15 rats (group 1) by streptozotocin injection and ligature induction. Periodontitis alone was produced by ligature induction in 15 rats (group 2), diabetes alone was produced by streptozotocin injection in 15 rats (group 3), and fifteen systemically and periodontally healthy rats were used as controls (group 4). The gingival TNF-α, IL-1β and LPS levels were measured by using ELISA method. Periodontal destruction was assessed by measuring the alveolar bone loss. Periodontal inflammation was quantified by histopathological grading in H&E stained samples.RESULTS: Higher levels of TNF-α, IL1-β and LPS, increased alveolar bone loss and more serve histopathology were found in group 1 compared with group 2, group 3 and group 4 (p < 0.05). The quantities of TNF-α, IL1-β and LPS, the amount of alveolar bone loss and the severity of the histopathological finding were greater in group 2 than group 3 and group 4 (p < 0.05). Group 3 demonstrated higher levels of TNF-α, IL1-β and LPS, increased alveolar bone loss and more serve histopathology than group 4 (p < 0.05). Statistically significant differences were noted between all of the groups.CONCLUSIONS: These data indicate that DM may lead to enhanced TNF-α, IL1-β and LPS production in the periodontal tissues. The resorption values of alveolar bone and the histological inflammation were more severe in rats with periodontitis and diabetes mellitus than in those with periodontitis alone, diabetes mellitus alone and control rats. Our findings are consistent with the hypothesis that hyperglycemia contributes to the heightened inflammatory response associated with periodontitis.

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Assessment of IL-1β and VEGF concentration in a rat model during orthodontic tooth movement and extracorporeal shock wave therapy

Cited by 25 publications

References 45 publications

Periodontal cytokines profile under orthodontic force and extracorporeal shock wave stimuli in a rat model

Periodontal cytokines profile under orthodontic force and extracorporeal shock wave stimuli in a rat model

Blocking of TRPV-1 in the parodontium relieves orthodontic pain by inhibiting the expression of TRPV-1 in the trigeminal ganglion during experimental tooth movement in rats

Study of TNF-α, IL-1βand LPS Levels in the Gingival Crevicular Fluid of a Rat Model of Diabetes Mellitus and Periodontitis

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