Assessment of indigenous fungal biocatalysts towards valorization of delignified physico‐chemically pretreated corn cobs and sugarcane bagasse

Rastogi, Meenal; Shrivastava, Smriti

doi:10.1002/bbb.2435

Cited by 5 publications

(1 citation statement)

References 47 publications

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“…However, direct enzymatic digestion allows higher glucose concentrations without worrying about the production of inhibitors. In contrast to bagasse and corn cob, okara can be directly enzymatically digested to monosaccharides (Rastogi and Shrivastava 2022 ). In the former, it is necessary to remove lignin from the raw material or destroy its structure before cellulase can act effectively (Cai et al 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Production of acetoin and its derivative tetramethylpyrazine from okara hydrolysate with Bacillus subtilis

Liu

Guo

et al. 2023

AMB Expr

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Okara, a renewable biomass resource, is a promising fermentative raw material for the bio-production of value-added chemicals due to its abundance and low-costs. we developed a process for the enzymatic hydrolysis of okara, and then engineered Bacillus subtilis to utilize mixed sugars to produce acetoin in okara hydrolysis without the addition of a supplemental nitrogen source. Okara was initially hydrolyzed with cellulase, β-glucosidase, and pectinase to obtain okara hydrolysate containing mixed sugars (32.78 ± 0.23 g/L glucose, 1.43 ± 0.064 g/L arabinose, 7.74 ± 0.11 g/L galactose) and amino acids. In this study, Bacillus subtilis 168 was used as the acetoin-producing strain, and the key genes bdhA and acoA of the acetoin catabolism pathway were knocked out to improve the fermentation yield of acetoin. In order to utilize the galactose in the hydrolysate, the recombinant strain BS03 (Bacillus subtilis168∆bdhA∆acoA) was used to overexpress the arabinose transporter-encoding gene (araE) drive heterologous expression of the Leloir pathway gene (galKTE). The corn dry powder concentration was optimized to 29 g/L in the reducing sugar okara hydrolysate. The results show that the recombinant bacterium BS03 could still synthesize 11.79 g/L acetoin without using corn dry powder as a nitrogen source. Finally, using okara enzymatic hydrolysate as the carbon and nitrogen source, 11.11 g/L and 29.7 g/L acetoin were obtained by batch fermentation and fed-batch fermentation, respectively, which was further converted to 5.33 g/L and 13.37 g/L tetramethylpyrazine (TTMP) by reaction with an ammonium salt.

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Section: Discussionmentioning

confidence: 99%

Production of acetoin and its derivative tetramethylpyrazine from okara hydrolysate with Bacillus subtilis

Liu

Guo

et al. 2023

AMB Expr

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Adsorption of extracellular lipase in a packed-bed reactor: an alternative immobilization approach

Freitas,

Remonatto,

Miotti Junior

et al. 2024

Bioprocess Biosyst Eng

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Application of the β-Glucosidase from the Fungus Kretzschmaria zonata on Sugarcane Bagasse Hydrolysis

Canettieri,

Pimentel,

Almeida

et al. 2024

Bioenerg. Res.

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β-glucosidases for industrial applications are mainly obtained from lamentous fungi. Kretzschmaria zonata is a phytopathogenic fungus that produces an arsenal of enzymes with biotechnological potential and this work aimed to produce, to purify and to characterize a β-glucosidase from the fungus K. zonata for its application in supplementation of a commercial cocktail for sugarcane bagasse sacchari cation.The higher speci c activity of β-glucosidase was induced by corn cob, 1.085 U/mg of protein. At the end of all puri cation steps, a puri cation factor of 6.52 was reached, with an increase of speci c activity from 1.22 U/mg, in the crude extract, to 7.97 U/mg. Concerning pH stability, at pH 4, the pH of maximal βglucosidase activity, the enzyme was completely stable, with 100% of activity after 1 h of incubation, while it kept over than 50% of activity in the pH range from 2.2 to 6. The optimum temperature was 60°C and he half-life times were estimated as 307.8 and 10 min, for temperatures of 60 and 70°C, respectively.The β-glucosidase showed a reduction in relative activity in the presence of 10 mM of manganese sulfate, zinc sulfate, manganese chloride, SDS and glucose, maintaining 55, 56, 62, 70, 73% of the relative activity, respectively. The commercial cocktail supplemented with the K. zonata β-glucosidase enabled the release of 13.89 g/L of glucose and 5.34 g/L of xylose, an increase of 19.8 and 35.5% of glucose and xylose release, respectively, after sugarcane bagasse hydrolysis.

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Assessment of indigenous fungal biocatalysts towards valorization of delignified physico‐chemically pretreated corn cobs and sugarcane bagasse

Cited by 5 publications

References 47 publications

Production of acetoin and its derivative tetramethylpyrazine from okara hydrolysate with Bacillus subtilis

Production of acetoin and its derivative tetramethylpyrazine from okara hydrolysate with Bacillus subtilis

Adsorption of extracellular lipase in a packed-bed reactor: an alternative immobilization approach

Application of the β-Glucosidase from the Fungus Kretzschmaria zonata on Sugarcane Bagasse Hydrolysis

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