Assessment of iron toxicity using a luminescent bacterial test with an Escherichia coli recombinant strain

Toxicity of Nine (Doped) Rare Earth Metal Oxides and Respective Individual Metals to Aquatic Microorganisms Vibrio fischeri and Tetrahymena thermophila

“…According to Storz and Imlay [ 50 ], the concentration of iron in the cells should be kept at low level as high concentrations of iron are toxic, mostly due to generation of reactive oxygen species (ROS). Sorokina et al [ 51 ] have reported 30-min EC 50 values for recombinant luminescent E. coli bacteria as 8.5 mg Fe 2+ /L and 1.3 mg Fe 3+ /L, i.e., quite similar to our data for V. fischeri (0.44 mg Fe 3+ /L) ( Table 2 ). Concerning toxicity of nickel, its toxic effect to microorganisms is considered to occur (i) due to binding of Ni to catalytic residues of enzymes and also inhibiting enzymes allosterically; (ii) replacing the essential metals from metalloproteins; and (iii) causing indirectly oxidative stress although Ni is considerably weak generator of oxidative damage when compared to iron or copper [ 52 ].…”

Cargo shuttling by electrochemical switching of core–shell microgels obtained by a facile one-shot polymerization

“…Although already the unloaded microgel P(NIPAM- co -VFc) is able to kill the model microbe S. aureus ATCC12600, which is attributed to the Fe( ii ) as previously reported in literature,102 loading with triclosan increases the killing efficiency. It is expected that the drug can diffuse from the microgel to a certain extent and kill bacteria based on the deformability and porosity of the microgel.…”

Comparative sensitivity of the luminescent Photobacterium phosphoreum, Escherichia coli, and Bacillus subtilis strains to toxic effects of carbon-based nanomaterials and metal nanoparticles

“…-10, 2010. Currently developers describe it as a lyophilized recombinant strain E. coli K12 TG1 (pF1) with a lux-CDABE cassette of the genes from V. fischeri MGU-6 (collection of the Lomonosov Moscow State University) stored under an inert gas (Sorokina et al, 2013). Application of this biosensor for the stated purpose was reported in a number of our previous works (Deryabin et al, 2011(Deryabin et al, , 2012.…”