Assessment of Keratinase and Other Hydrolytic Enzymes in Thermophilic Bacteria Isolated from Geothermal Areas in Patagonia Argentina

Cavello, Ivana Alejandra; Urbieta, María Sofía; Segretin, Ana Belén; Giaveno, Alejandra; Cavalitto, Sebastián Fernando; Donati, Edgardo R.

doi:10.1080/01490451.2017.1339144

Cited by 23 publications

(18 citation statements)

References 57 publications

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“…Moreover, protease activities of Paenibacillus species were screened in this study. However, there are a few studies about protease of Paenibacillus isolated from thermophilic area [30][31][32][33]. Besides, there is no study about protease of Paenibacillus lactis.…”

Section: Discussionmentioning

confidence: 99%

“…Production abilities of hydrolytic enzymes including keratinases, proteases, esterases, amylases, cellulases, inulinases, pectinases and xylanases from thermophilic isolates were investigated in study. 83% of screened isolates containing Paenibacillus dendritiformis was reported to have protease activity [32]. Panosyan investigated production abilities of hydrolytic enzymes from thermophilic bacilli including Paenibacillus genus.…”

Section: Discussionmentioning

confidence: 99%

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Thermophilic Prokaryotic Diversity of Eynal Geothermal Spring and Protease Production Potentials of Isolates

Erdönmez¹,

Gedikli

Yaman³

et al. 2020

Eskişehir Technical University Journal of Science and Technology a - Applied Sciences and Engineering

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Thermophilic microorganisms are quite attractive for the study of biodiversity and evolutionary process as well as biotechnological applications. These organisms provide significant advantages for industrial and biotechnological processes occurring fast and efficiently at high temperatures. Possible potential also is getting increase thanks to isolation of new strains, determination of new metabolites and their pathway. Within the scope of this study, thermophilic bacterial community was investigated with a combination of classical microbiology and molecular biology approaches including fluorescent in situ hybridization, amplified ribosomal DNA restriction analysis, and polymerase chain reaction of 16S rRNA gene. Archaea and Bacteria domain were screened by Fluorescence in situ Hybridization technique. At the end of culture-dependent methodology, Paenibacillus lactis E3.1 (MK573857), Brevibacillus borstelensis E3.2 (MK573871), Paenibacillus naphthalenovorans E2.2 (MK573627), Paenibacillus sp. E3.5 (MK573870) were obtained. Furthermore, these isolates were screened with regards to protease production capabilities. At the end of screening studies, the highest protease activity (300 U/mg) was observed for Paenibacillus lactis E3.1.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Thermophilic Prokaryotic Diversity of Eynal Geothermal Spring and Protease Production Potentials of Isolates

Erdönmez¹,

Gedikli

Yaman³

et al. 2020

Eskişehir Technical University Journal of Science and Technology a - Applied Sciences and Engineering

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“…On the basis of phylogenetic analysis, the strains 1S and 4S were closely related to type strain B. subtilis subsp. inaquosorum (CP013984) with 99.92 and 100% respectively 29,46 . The sequencing results revealed that the genus Bacillus was predominant rather than Geobacillus and Aeribacillus in sand dunes of Thar Desert.…”

Section: Journal Of Pure and Applied Microbiologymentioning

confidence: 99%

Characterization, Phylogenetic Analysis and Potential Applications of Heterotrophic Bacteria Inhabit Sand Dunes of Thar Desert, India

Neelam¹,

Agrawal²,

Tomer³

et al. 2018

J Pure Appl Microbiol

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Microorganisms inhabit extreme environments are novel in terms of phylogeny and also valuable source of extracellular hydrolytic enzymes. The present study focused on isolation and characterization of moderately thermophilic heterotrophic bacteria from Thar Desert using culture dependent method and assessment of their potential for production of industrially important enzymes. Screening of bacteria from sand dune samples collected from arid region of India led to isolation of total eight moderately thermophilic bacterial strains. All strains were characterized morphologically, biochemically and 16S rRNA gene sequencing analysis. The phylogenetic analysis of strains revealed that these strains affiliated to genera Bacillus (5 strains), Geobacillus (2 strains) and Aeribacillus (1 strain), which were able to grow between 25-60 °C. The present study documented the occurrence of species Geobacillus thermodenitificans, Bacillus paralicheniformis and Aeribacillus pallidus in sand dunes of Thar Desert, India. These moderately thermophilic strains showed an ability to produce one or more extracellular enzymes like amylase, protease, lipase and asparaginase. Thus, the strains isolated from Thar Desert could be potential candidates for industrial applications. The present study showed that bacterial strains exist in the same ecological niche might have ability to produce distinct enzymes.

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“…Patagonia in the southwest of Argentina presents two volcanic geothermal areas-Copahue and Domuyo. The Copahue geothermal area, predominantly acidic, has been extensively studied as for its prokaryotic biodiversity [2][3][4] and the biotechnological applications of some of their species [5][6][7]. On the other hand, the Domuyo geothermal area remains almost unexplored in both aspects.…”

Section: Introductionmentioning

confidence: 99%

Bacillus cytotoxicus Isolated from a Pristine Natural Geothermal Area Reveals High Keratinolytic Activity

et al. 2020

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Geothermal areas are the niches of a rich microbial diversity that is not only part of the intangible patrimony of a country but also the source of many microbial species with potential biotechnological applications. Particularly, microbial species in geothermal areas in Argentina have been scarcely explored regarding their possible biotechnological uses. The purpose of this work was to explore the proteolytic and keratinolytic enzymatic potential of microorganisms that inhabit in the Domuyo geothermal area in the Neuquén Province. To this end, we did enrichment cultures from two high-temperature natural samples in mineral media only supplemented with whole chicken feathers. After the isolation and the phylogenetic and morphologic characterization of different colonies, we obtained a collection of Bacillus cytotoxicus isolates, a species with no previous report of keratinolytic activity and only reported in rehydrated meals connected with food poisoning outbreaks. Its natural habitat has been unknown up to now. We characterized the proteolytic and keratinolytic capacities of the B. cytotoxicus isolates in different conditions, which proved to be remarkably high compared with those of other similar species. Thus, our work represents the first report of the isolation as well as the keratinolytic capacity characterization of strains of B. cytotixicus obtained from a natural environment.

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Assessment of Keratinase and Other Hydrolytic Enzymes in Thermophilic Bacteria Isolated from Geothermal Areas in Patagonia Argentina

Cited by 23 publications

References 57 publications

Thermophilic Prokaryotic Diversity of Eynal Geothermal Spring and Protease Production Potentials of Isolates

Thermophilic Prokaryotic Diversity of Eynal Geothermal Spring and Protease Production Potentials of Isolates

Characterization, Phylogenetic Analysis and Potential Applications of Heterotrophic Bacteria Inhabit Sand Dunes of Thar Desert, India

Bacillus cytotoxicus Isolated from a Pristine Natural Geothermal Area Reveals High Keratinolytic Activity

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