Background: Birth asphyxia, which is defined as the failure to establish breathing following delivery and causes an estimated 900,000 deaths each year. This investigation tries to measure the efficiency of an instructional program on the knowledge of pediatric nurses concerning neonatal birth asphyxia management in Mosul Hospitals.The Methodology: A quasi-experimental one-group pretest and posttest study design was used at Mosul hospitals' pediatric units from 3rd October/2021 to 1st March/2022. A not-randomly purposive sample was used, the whole number of sample (32) nurses, drawn from five Mosul City hospitals. Nurses were given access to the program lectures and the questionnaire. Five lectures made up the program's five lectures, and then questionnaire remained divided into two parts. The initial part of the questionnaire asked about socio-demographic data, while the part second asked about the knowledge of nurses on the management of newborn birth asphyxia and included five components. The validity was examined by thirteen expert committees. The pilot study's dependability was used from 16-17/November/2021 by use of Cronbach's Alpha (0. 831) using SPSS version 26.The Result: Most of the sample was females at age (20-29) years-old, who graduated from the institute stage had less than five years of service, and not had training courses, and also reading resources related to neonatal birth asphyxia. There was the extremely substantial correlation between the pediatric nurse's knowledge of newborn birth asphyxia between the Pretest with Posttest results demonstrates the educational program's success at P. value ≤ 0.05.The Recommendations: Widespread training courses, also special programs, and necessity be designed and implemented in other Nineveh hospitals.