Assessment of Lead Toxicity Using Drosophila melanogaster as a Model

Mathew, Blessy Baby; Krishnamurthy, NB

doi:10.4172/2161-0495.1000380

Cited by 14 publications

(8 citation statements)

References 16 publications

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“…In the current study, short-term exposure of adult D. melanogaster to a sublethal concentration of CdO NPs adversely affected all studied life history traits of the fly including survival, developmental time and reproductive fitness. Such results are in line with previous studies revealing that exposure to Cd, either as salts (CdCl2) or as nanosized (CdO) can cause toxic effects in D. melanogaster as well as organisms (AL Naggar et al, 2018;Bixler and Schnee, 2018;Blum et al, 2012;Dabour et al, 2019;Hu et al, 2019;Mathew and NB, 2018). Inhalation of CdO NPs during pregnancy negatively affects fecundity and inhibits the development of fetal and postnatal offspring of mice (Blum et al, 2012).…”

Section: Discussionsupporting

confidence: 89%

“…The copyright holder for this preprint (which this version posted July 16, 2020. ; https://doi.org/10.1101/2020.07.15.204370 doi: bioRxiv preprint even at lesser concentrations for short durations (Bixler and Schnee, 2018; Mathew and NB, 2018), because it might negatively affect expressions of genes associated with reproduction of D. melanogaster and trigger the transcription of defense-related genes (Hu et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

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Consequences of a short-term exposure to a sub lethal concentration of CdO nanoparticles on key life history traits in the fruit fly (Drosophila melanogaster)

Kholy

Giesy

Naggar

2021

Journal of Hazardous Materials

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Nanoparticles of cadmium oxide (CdO NPs) are among the most common industrial metal oxide nanoparticles. Early adulthood (F0) fruit flies (D. melanogaster) were exposed for 7 days to a sub lethal concentration (0.03 mg CdO NPs/ml, which was 20% of the LC50), spiked into food media to test for long term-effects over time and beyond their direct exposure on key life history traits . Effects on survival, developmental time, eclosion rate, fecundity and negative geotaxis performance were assessed. Potential effects on ultrastructure of mid gut cells were also investigated by use of electron microscopy. All studied life history traits, as well as climbing behavior were adversely affected by exposure to CdO NPs. In non-exposed progeny (F1) of adult flies (F0), a blistered wing phenotype was also observed. Lysis of nuclear and rough endoplasmic reticulum (rER) membranes, mitochondrial swelling and lysis were among the most common cellular alterations observed in midgut cells of F0 flies exposed to CdO NPs.Genes encoding for metallothionein (MTn A-D) were significantly upregulated in both parent flies (F0) and their progeny (F1) after exposure of F0 flies to CdO NPs, compared to unexposed, control flies, a result which indicated potential, long-term effects. Taken together, these results suggest that short-term exposure to a sublethal concentration of CdO NPs is sufficient to cause long-lasting, harmful effects on fruit flies.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Consequences of a short-term exposure to a sub lethal concentration of CdO nanoparticles on key life history traits in the fruit fly (Drosophila melanogaster)

Kholy

Giesy

Naggar

2021

Journal of Hazardous Materials

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show abstract

“…In the current study, short-term exposure of adult D. melanogaster to a sublethal concentration of CdO NPs adversely affected all studied life history traits of the fly including survival, developmental time and reproductive fitness. Such results are in line with previous studies revealing that exposure to Cd, either as salts (CdCl2) or as nano-sized (CdO) can cause toxic effects in D. melanogaster as well as organisms (AL Naggar et al, 2018; Bixler and Schnee, 2018; Blum et al, 2012; Dabour et al, 2019; Hu et al, 2019; Mathew and NB, 2018). Inhalation of CdO NPs during pregnancy negatively affects fecundity and inhibits the development of fetal and postnatal offspring of mice (Blum et al, 2012).…”

Section: Discussionsupporting

confidence: 92%

“…Here, the flies exposed to CdO NPs by oral intake and long-lasting effects beyond direct exposure times were further investigated by assessing the fecundity of both parents (F 0 ) flies and its non-treated progeny (F 1 ) flies where, the fecundity of F 1 flies were significantly reduced (−50%) compared with fecundity of unexposed F 1 flies. These findings also match the results of previous studies, which indicated that the presence of Cd in the environment may impair the fitness of D. melanogaster adulthood, even at lesser concentrations for short durations (Bixler and Schnee, 2018; Mathew and NB, 2018), because it might negatively affect expressions of genes associated with reproduction of D. melanogaster and trigger the transcription of defense-related genes (Hu et al, 2019).…”

Section: Discussionsupporting

confidence: 91%

Consequences of a short-term exposure to a sub lethal concentration of CdO nanoparticles on key life history traits in the fruit fly (Drosophila melanogaster)

El-Kholy

Giesy

Naggar

2020

Preprint

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Nanoparticles of cadmium oxide (CdO NPs) are among the most common industrial metal oxide nanoparticles. Early adulthood (F0) fruit flies (D. melanogaster) were exposed for 7 days to a sub lethal concentration (0.03 mg CdO NPs/ml, which was 20% of the LC50), spiked into food media to test for long term-effects over time and beyond their direct exposure on key life history traits. Effects on survival, developmental time, eclosion rate, fecundity and negative geotaxis performance were assessed. Potential effects on ultrastructure of mid gut cells were also investigated by use of electron microscopy. All studied life history traits, as well as climbing behavior were adversely affected by exposure to CdO NPs. In non-exposed progeny (F1) of adult flies (F0), a blistered wing phenotype was also observed. Lysis of nuclear and rough endoplasmic reticulum (rER) membranes, mitochondrial swelling and lysis were among the most common cellular alterations observed in midgut cells of F0 flies exposed to CdO NPs. Genes encoding for metallothionein (MTn A-D) were significantly upregulated in both parent flies (F0) and their progeny (F1) after exposure of F0 flies to CdO NPs, compared to unexposed, control flies, a result which indicated potential, long-term effects. Taken together, these results suggest that short-term exposure to a sublethal concentration of CdO NPs is sufficient to cause long-lasting, harmful effects on fruit flies.Graphical Abstract

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“…Lead (Pb) is one of the most widely spread heavy metals in nature; it has a significant effect on different life-history traits of Drosophila species [11][12][13]. Increased concentrations of Pb were observed to significantly affect D. melanogaster in their developmental stages, causing a decrease in the percentage of hatching [14]. It remains to be seen to what extent the interaction between microorganisms and their host is part of an adaptive response to lead pollution.…”

Section: Introductionmentioning

confidence: 99%

Altered diversity of bacterial communities in two Drosophila species under laboratory conditions and lead exposure

Beribaka

Dimkić

Jelić

et al. 2021

ARCH BIOL SCI BELGRA

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The composition of microbiota affects different traits of Drosophila throughout its life cycle and represents an important part of the adaptive response to environmental changes, such as increased levels of accumulated heavy metals in their habitats. We investigated the effects of lead added to the feeding substrate on the microbiota diversity in the fruit flies, Drosophila melanogaster and Drosophila subobscura. We compared the bacterial diversity of wild-caught flies and their progeny reared under standard laboratory conditions. We analyzed the shifts in bacterial diversity in the experimental groups of flies reared for 13 generations on standard and lead(II) acetate (Pb(CH3COO)2), Pb acetate-saturated substrates. Identification of the main bacterial genera was performed by next-generation sequencing (NGS) of the V3-V4 variable regions of the 16S rRNA gene. Results indicate significant species-specific differences in bacterial composition between natural and laboratory populations, and between the substrates. Diversity increased in both species under prolonged exposure to lead-polluted substrate, suggesting a potential adaptive response to an environmental stress.

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Assessment of Lead Toxicity Using Drosophila melanogaster as a Model

Cited by 14 publications

References 16 publications

Consequences of a short-term exposure to a sub lethal concentration of CdO nanoparticles on key life history traits in the fruit fly (Drosophila melanogaster)

Consequences of a short-term exposure to a sub lethal concentration of CdO nanoparticles on key life history traits in the fruit fly (Drosophila melanogaster)

Consequences of a short-term exposure to a sub lethal concentration of CdO nanoparticles on key life history traits in the fruit fly (Drosophila melanogaster)

Altered diversity of bacterial communities in two Drosophila species under laboratory conditions and lead exposure

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