Assessment of leukemia inhibitory factor levels by uterine flushing at the time of egg retrieval does not adversely affect pregnancy rates with in vitro fertilization

Olivennes, F.; Lédée-Bataille, Nathalie; Samama, Marise; Kadoch, Jacques; Taupin, Jean‐Luc; Dubanchet, Sylvie; Chaouat, Gérard; Frydman, René

doi:10.1016/s0015-0282(02)04949-x

Cited by 42 publications

(21 citation statements)

References 35 publications

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“…8,10,28 In contrast, no decrease in pregnancy rates have been observed following transcervical aspiration of endometrial secretion fluids immediately before embryo transfer. 8,10,28 This technique can be applied during the window of implantation without disrupting the implantation process, 8,10,28 and is associated with less discomfort than endometrial suction microbiopsy. 9 Moreover, a single aspiration sample from 1 cm above the internal os and a single tissue biopsy was previously observed in hysterectomy specimens to be representative of other areas sampled within the uterine cavity (H. Beier, pers.…”

Section: Discussionmentioning

confidence: 99%

“…This may be because of the large range of the LIF concentrations observed in endometrial secretions. 10,16,20,44 Furthermore, there is evidence that LIF expression is not primarily modulated by steroid hormones, 45 but by transforming growth factor-b, tumour necrosis factor-a and interleukin 1b. 46 Although LIF has been shown to be crucial for implantation in mice, 36 its importance and regulation pathways in human implantation remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Aspiration and flushing of the endometrial cavity in the peri-implantation period of menstrual cycles has been performed without detrimental effect on pregnancy rates. [8][9][10] Analysis of endometrial secretions showed proteins originating from transudate of serum, leakage products of apoptotic epithelial cells, and glandular secretion. 11 The composition of the secretions varies during the menstrual cycle as a result of changing ovarian steroid levels.…”

Section: Introductionmentioning

confidence: 99%

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The feasibility of a less invasive method to assess endometrial maturation—comparison of simultaneously obtained uterine secretion and tissue biopsy

Gaast¹,

Macklon²,

Beier-Hellwig³

et al. 2008

BJOG

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Objective To compare the assessment of endometrial maturation parameters in endometrial secretion samples obtained by a novel minimally invasive technique with those assessed in tissue biopsies.Design Prospective study.Setting University Hospital.Population Healthy female volunteers attending a gynaecological outpatient clinic.Methods Endometrial secretion fluid and tissue sampling 5 days after a spontaneous ovulation assessed with ultrasound.Main outcome measures Progesterone (P) receptor, Ki-67 expression and the Noyes criteria were used to date endometrial biopsies. In the endometrial fluid samples, glycodelin A (GdA), leukaemia inhibitory factor (LIF) and P levels were analysed, and protein content and electrophoresis patterns were determined.Results All data were correlated to estradiol (E 2 ) and P serum concentrations. The dating according to histology and immunohistochemical staining patterns correlated significantly with GdA levels (r = 0.376, P = 0.048) in endometrial fluid samples as well with serum levels of E 2 (r = 0.568, P = 0.001) and P (r = 0.408, P = 0.023). No correlation was observed between tissue dating and LIF levels and protein content in endometrial fluid samples.Conclusions The measurement of GdA in endometrial secretion samples may provide a less invasive method for assessing endometrial maturation in potential conception cycles without disrupting implantation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The feasibility of a less invasive method to assess endometrial maturation—comparison of simultaneously obtained uterine secretion and tissue biopsy

Gaast¹,

Macklon²,

Beier-Hellwig³

et al. 2008

BJOG

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“…We then instill 1 ml of saline water immediately and gently aspirate it and repeat these two steps again. Innocuity of the method has been previously documented [12] . In contrast to previous studies, we did not discard the samples with endometrium.…”

Section: Uterine Flushingmentioning

confidence: 99%

Uterine Receptivity and Cytokines: New Concepts and New Applications

Lédée¹,

Dubanchet²,

Oger³

et al. 2007

Gynecol Obstet Invest

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The implantation process, currently thought to be the most critical step in achieving a successful early pregnancy, remains one of the most important unsolved processes in reproductive medicine. It depends on uterine-dependent and embryo-specific events, which need to be critically coordinated. Early embryo signaling following a maternal hormonal or cytokine-mediated preparation phase seems to be involved in stages immediately before, during and just after the apposition step to permit adequate proliferation of the stroma. Our objective is to develop guidelines and diagnostic tools pertinent to appreciate uterine receptivity. We will focus our attention on the uterine luminal environment at the time of oocyte retrieval and on the monitoring of the endometrium using three-dimensional ultrasound associated with digital technology and cytokine quantification by real-time PCR during the implantation window in an IVF/ICSI population. There is an accumulating body of data which strongly suggests that both implantation and uterine receptivity are controlled, primarily, though not exclusively, by locally acting growth factors and cytokines, some under steroid control. Some specific cytokines (IL-12, IL-15 and IL-18) in the luminal environment and in the endometrium allow a distinct pattern of abnormal uterine receptivity. The identification of these distinct patterns of abnormal uterine receptivity and of the mechanisms leading to the abnormal angiogenesis before implantation strongly suggest that no single therapeutic scheme can correct all cases of implantation failure and should be adapted for each patient especially in the case of unexplained infertility.

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“…Studies on IUI/IVF have demonstrated that in addition to the oocyte age (6), uterine age (7-9) also plays contributory role in age-related decline in fertility. Although not precisely explored, uterine factors like hexose, protein (10), fibrinolytic enzymes (11), chemotaxis (12), and others do contribute a fundamental role in the process of implantation (13,14).…”

mentioning

confidence: 99%

Role of an estrogen-upregulated 64.0-kDa uterine fluid glycoprotein in improving fertility in women

Das

Chattopadhyay

Ghosh

et al. 2007

Fertility and Sterility

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Assessment of leukemia inhibitory factor levels by uterine flushing at the time of egg retrieval does not adversely affect pregnancy rates with in vitro fertilization

Cited by 42 publications

References 35 publications

The feasibility of a less invasive method to assess endometrial maturation—comparison of simultaneously obtained uterine secretion and tissue biopsy

The feasibility of a less invasive method to assess endometrial maturation—comparison of simultaneously obtained uterine secretion and tissue biopsy

Uterine Receptivity and Cytokines: New Concepts and New Applications

Role of an estrogen-upregulated 64.0-kDa uterine fluid glycoprotein in improving fertility in women

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