Assessment of limbus and central cornea in patients with keratolimbal allograft transplantation using in vivo laser scanning confocal microscopy: an observational study

Hong, Jiaxu; Zheng, Tianyu; Xu, Jianjiang; Deng, Sophie X.; Chen, Ling; Sun, Xinghuai; Le, Qihua; Li, Yimin

doi:10.1007/s00417-011-1616-x

Cited by 14 publications

(22 citation statements)

References 28 publications

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“…Seeing firsthand the health of the epithelial tissue at a cellular level can provide a better evaluation of the viability of the graft and the recovery of normal corneal epithelium. 28 …”

Section: Discussionmentioning

confidence: 99%

Limbal Basal Cell Density Decreases in Limbal Stem Cell Deficiency

Chan¹,

Chen²,

Rao³

et al. 2015

American Journal of Ophthalmology

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Purpose To investigate changes in limbal basal epithelial cell density in eyes with limbal stem cell deficiency (LSCD) using in vivo confocal laser scanning microscopy Design retrospective observational comparative study Methods A total of 43 eyes of 30 patients diagnosed with LSCD were included in the study. Ten eyes from normal subjects were included as control. Confocal imaging of the central cornea, and the superior, nasal, inferior and temporal limbus were collected using the Heidelberg Retina Tomograph III Rostock Corneal Module. Basal cell density in all locations was measured by two independent observers. Results The mean basal cell density of the normal group was 9264 ±598 cells/mm2 in the cornea and 7120 ±362 cells/mm2 in the limbus. In the LSCD group, the mean basal cell density in the cornea decreased 31.0% (6389 ±1820 cells/mm2, p<0.001) and in the limbus decreased 23.6% (5440 ±1123 cells/mm2, p<0.001) compared to that in the control. There was a trend of basal cell density decline in more advanced stage of LSCD. The basal cell density declined in the unaffected regions at a similar degree as that in the affected region in sectoral LSCD (p>0.05). The basal cell diameter increased by 24.6% in the cornea (14.7 μm) and by 15.7% in the limbus (15.5 μm) compared to the control. Conclusions Basal cell density in both central cornea and limbus decreases in LSCD. LSCs are affected globally and basal cell density could be used as a parameter to measure LSC function at the early stages of the disease process.

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“…Seeing firsthand the health of the epithelial tissue at a cellular level can provide a better evaluation of the viability of the graft and the recovery of normal corneal epithelium. 28 …”

Section: Discussionmentioning

confidence: 99%

Limbal Basal Cell Density Decreases in Limbal Stem Cell Deficiency

Chan¹,

Chen²,

Rao³

et al. 2015

American Journal of Ophthalmology

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Section: The Limbal Stem Cells and The Stem Cell Nichementioning

confidence: 99%

“…32 Moreover, IVCM has shown that loss of the POV correlates with loss of central and limbal basal epithelial cells and an increase in limbal dendritic cells. 32 The corneal or conjunctival epithelial transition zone and cellular phenotype as revealed by IVCM have also been shown in correlation with the presence or absence of the POV in LSCD. 33,34 In another series of LSCD patients, IVCM was used to examine the POV and limbal epithelium in early, intermediate, and late clinical stages of LSCD, where one could see that the POV were completely degraded at the early stage.…”

Section: The Limbal Stem Cells and The Stem Cell Nichementioning

confidence: 99%

Congenital Aniridia and the Ocular Surface

et al. 2016

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Aniridia is a congenital pan-ocular disorder caused by haplo-insufficiency of Pax6, a crucial gene for proper development of the eye. Aniridia affects a range of eye structures, including the cornea, iris, anterior chamber angle, lens, and fovea. The ocular surface in particular can be severely affected by a progressive pathology termed aniridiaassociated keratopathy (AAK), markedly contributing to impaired vision. The purpose of this review is to provide an update of the current knowledge of the genetic, clinical, micromorphological, and molecular aspects of AAK. We draw upon material presented in the literature and from our own observations in large aniridia cohorts. We summarize signs and symptoms of AAK, describe current options for management, and discuss the latest research 2 findings that may lead to better diagnosis and new treatment or prevention strategies for this debilitating ocular surface condition.

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“…The limbus contains radially oriented fibro vascular ridges, known as the palisades of Vogt (POV), that are interspaced by epithelial rete ridges or so called limbal epithelial crypts [8,9]. The structure of the POV is as unique as fingerprints, and their shape, size and spatial distribution vary over time with age, as well as in response to medication, surgery or congenital conditions [10][11][12][13]. The limbal epithelial crypts house stem cells, which play a key role in the regeneration of the corneal epithelium [6,7].…”

Section: Introductionmentioning

confidence: 99%

In-vivo imaging of the palisades of Vogt and the limbal crypts with sub-micrometer axial resolution optical coherence tomography

Bizheva¹,

Tan²,

MacLellan³

et al. 2017

Biomed. Opt. Express

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A research-grade OCT system was used to image in-vivo and without contact with the tissue, the cellular structure and microvasculature of the healthy human corneo-scleral limbus. The OCT system provided 0.95 µm axial and 4 µm (2 µm) lateral resolution in biological tissue depending on the magnification of the imaging objective. Cross-sectional OCT images acquired tangentially from the inferior limbus showed reflective, loop-like features that correspond to the fibrous folds of the palisades of Vogt (POV). The high OCT resolution allowed for visualization of individual cells inside the limbal crypts, capillaries extending from the inside of the POV's fibrous folds and connecting to a lateral grid of micro-vessels located in the connective tissue directly below the POV, as well as reflections from individual red blood cells inside the capillaries. Difference in the reflective properties of the POV was observed among subjects of various pigmentation levels of the POV. Morphological features observed in the high resolution OCT images correlated well with histology. The ability to visualize the limbal morphology and microvasculature in-vivo at cellular level can aid the diagnostics and treatment of limbal stem cell dysfunction and dystrophies. a novel anatomical structure and a putative limbal stem cell niche," Br.

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Assessment of limbus and central cornea in patients with keratolimbal allograft transplantation using in vivo laser scanning confocal microscopy: an observational study

Abstract: In vivo LSCM is a useful tool for monitoring the cellular changes in KLAL grafts, and has the potential to diagnose the failure of KLAL grafts at the cellular level.

Cited by 14 publications

References 28 publications

Limbal Basal Cell Density Decreases in Limbal Stem Cell Deficiency

Limbal Basal Cell Density Decreases in Limbal Stem Cell Deficiency

Congenital Aniridia and the Ocular Surface

In-vivo imaging of the palisades of Vogt and the limbal crypts with sub-micrometer axial resolution optical coherence tomography

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