Assessment of lipid peroxidation and antioxidant status in rheumatoid arthritis and osteoarthritis patients

El-Barbary, Amal Mohamad; Khalek, Manal Aly Abdel; Elsalawy, Alaa M; Hazaa, Sahar

doi:10.1016/j.ejr.2011.07.002

Cited by 68 publications

(41 citation statements)

References 43 publications

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“…Furthermore, catalase activity significantly decreased in RA patients which is in line with the study performed by Sarban et al 12 and El-barbary et al 5 . A similar decrease of CAT activity was also detected in the synovial fluid of RA patients as reported by seven et al 13 .…”

Section: Discussionsupporting

confidence: 80%

“…Our data demonstrate that the activity of erythrocyte SOD is unchanged in patients with RA compared to healthy individuals which is in line with a previous study performed by Sarban et al 12 . However, it is undeniable that further studies done on patients with RA have shown a decreased erythrocyte SOD activity in their patient's cohort 5,13 . This lack of results reproducibility from one study to another could be explained by the intervention of environmental factors in the onset of the disease between one population to another and also by the size of the study cohort which without any doubt could play an important role in the interpretation of the results.…”

Section: Discussionmentioning

confidence: 99%

“…In fact, many studies have shown that the antioxidant system was impaired in patients [4][5] and oxidative stress markers were increased in patients with RA 6,7 . To the best of our knowledge, this is the first study performed in Tunisian patients with RA and the largest RA study population actually analysed for oxidative stress parameter correlations.…”

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confidence: 99%

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2016

IJPSR

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Rheumatoid arthritis is a chronic inflammatory disease with several triggers such as oxidative stress. The aim of this study was to assess the antioxidant enzyme activities (superoxide dismutase, glutathione peroxidase, glutathione reductase and catalase), the total antioxidant status, lipid peroxidation (malondialdehyde) and homocysteine levels in patients with rheumatoid arthritis. The study population consists of 172 patients with rheumatoid arthritis and 147 healthy controls. The glutathione peroxidase and the glutathione reductase erythrocyte activities were determined at 340 nm. The catalase activity was measured by colorimetric assay (Góth). The superoxide dismutase activity and the total antioxidant status levels were calculated using the colorimetric method at 505 nm and 600 nm respectively. Plasma malondialdehyde was determined by the spectrofluorometric method of Yagi. Serum homocysteine was measured by fluorescence polarization immunoassay. Glutathione peroxidase, glutathione reductase and catalase activities and total antioxidant status levels were significantly lower in patients compared to controls. However, malondialdehyde and homocysteine levels in patients were significantly higher than in healthy subjects. No significant difference in superoxide dismutase activity between patients and healthy subjects was noticed. Decreased antioxidant system and increased malondialdehyde and homocysteine in Tunisian rheumatoid arthritis patients confirm the implication of the oxidative stress in rheumatoid arthritis and suggest the role of homocysteine as a pro-oxidant factor.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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2016

IJPSR

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“…Available data only suggested a possible relationship between lipid peroxidation products, such as malondialdehyde, and disease activity in patients with RA. 2,[26][27][28] Based on our findings, we may assume that serum 4-HNE levels in RA patients are not directly correlated with inflammatory activation in patients with RA. Controversial results in the literature may stem from complex mechanism of action of lipid peroxidation involving more than one step in the pathogenetic process of inflammation.…”

Section: Discussionmentioning

confidence: 90%

Is 4-Hydroxynonenal a Predictive Parameter for the Development of Joint Erosion in Patients With Rheumatoid Arthritis?

et al. 2016

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Objectives: This study aims to evaluate serum 4-hydroxynonenal (4-HNE) levels and its clinical and radiological significance in patients with rheumatoid arthritis (RA). Patients and methods:The study included 40 patients (8 males, 32 females; mean age 51.4±11.2 years; range 24 to 72 years) with RA and 30 healthy controls (8 males, 32 females; mean age 53.0±11.7 years; range 24 to 72 years. Serum 4-HNE levels were measured using sandwich enzyme-linked immunosorbent assay method. Patients with disease activity score 28 ≤3.2 and >3.2 were allocated into low and high/moderate disease activity groups, respectively. Additionally, patients were divided into two groups as early RA (disease duration ≤2 years) and established RA (disease duration ≥2 years). Functional disability was evaluated using health assessment questionnaire. Radiographs were scored using the modified Larsen scoring. Results: Serum 4-HNE levels in patients with RA were significantly higher than controls (p=0.001). Serum 4-HNE levels did not correlate with laboratory or clinical parameters of disease activity including erythrocyte sedimentation rate, C-reactive protein, disease activity score 28, and health assessment questionnaire. Serum 4-HNE levels were higher in patients with established RA than patients with early RA (r=0.487, p=0.001). Besides, modified Larsen score which indicates structural damage correlated significantly with serum 4-HNE levels (p=0.001). Conclusion: These results indicate that serum 4-HNE levels may be used as an indicator for structural damage such as erosions in the early stage of RA; however, they are not efficient to monitor disease activity.

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“…The increased plasma MDA concentration in RA patients could be due to excessive generation of ROS which progresses in chronic inflammation causing tissue damage. 2,5,25,[29][30][31][32] The current study demonstrated that plasma MDA was positively correlated with titer of rheumatoid factor in the RA patients, while plasma TAP was negatively correlated with OSI and TPP. The established negative correlations observed in the current study suggest that RA patients are prone oxidative stress which leads to decreased total antioxidant capacity and increased lipid peroxidation products (MDA).…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress factors in Nigerians with rheumatoid arthritis

Yeldu

Arinola

2017

Int J Res Med Sci

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Background: Rheumatoid arthritis (RA) is a chronic progressive inflammatory autoimmune disorder characterized by symmetric erosive synovitis and sometimes with multi-system involvement. But the exact mechanism of the disease is not fully understood. In the light of above explanation, the present study measured the plasma levels of total peroxide potential (TPP), total antioxidant potential (TAP), malondialdehyde (MDA), oxidative stress index (OSI) and nitric oxide (NO) in relation to the titer of rheumatoid factor among RA patients compared with controls.Methods: This study included 28 rheumatoid arthritis patients and 28 apparently healthy subjects as controls who were matched for age (50-60 years), sex, and socioeconomic status. Rheumatoid factor was estimated using latex method as described by manufacturer. Anthropometric parameters and plasma levels of TPP, TAP, OSI, MDA and NO were determined using standard techniques.Results: The result indicated that with the exception of mean body weight which was significantly (p<0.001) higher among RA patients (90.61±2.02 years) as compared with controls (77.91±2.51 years), mean age, height and body mass index of RA patients (55.68±1.05kg, 1.65±0.01m and 33.40±0.83 kg/m2 respectively) were not significantly different compared with controls (54.07±1.04kg, 1.61±0.02m and 30.44±1.28 kg/m2 respectively). Plasma TPP, NO, OSI and MDA were significantly (p<0.01; p<0.001) higher while, plasma TAP is significantly lower among RA patients compared with controls. Plasma MDA was positively correlated with titer of rheumatoid factor in the RA patients.Conclusions: Our findings therefore may raise the concept that there are some yet unknown key events in the pathogenesis of RA determination of sex of the skull along with other parameters.

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Assessment of lipid peroxidation and antioxidant status in rheumatoid arthritis and osteoarthritis patients

Cited by 68 publications

References 43 publications

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Is 4-Hydroxynonenal a Predictive Parameter for the Development of Joint Erosion in Patients With Rheumatoid Arthritis?

Oxidative stress factors in Nigerians with rheumatoid arthritis

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