Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges

Tsikas, Dimitrios

doi:10.1016/j.ab.2016.10.021

Cited by 1,456 publications

(948 citation statements)

References 107 publications

Supporting

Mentioning

891

Contrasting

Unclassified

Order By: Relevance

“…This issue has been noted previously: even when a single batch of samples was prepared and distributed to several laboratories for analysis; in this study, malondialdehyde was found to have the lowest inter-laboratory variability compared to HNE and isoprostanes [84]. The analysis and levels of MDA and HNE in clinical samples have been discussed in an extensive recent review [83], and the same author also reported that sample storage time can significantly affect the concentrations [130]. In general, no or only a small (approximately 2-fold) increase in plasma aldehyde concentration is seen in disease, although some of these are statistically significant.…”

Section: Quantification Of Aldehydes In Human Plasma or Serummentioning

confidence: 54%

“…Methods for the detection of free alkanals, 2-alkenals, 2,4-alkadienals, 4-hydroxyalkenals and their quantification include spectrophotometric methods and gas or liquid chromatography coupled to mass spectrometry [82,83]. The simplest approaches depend on direct spectrophotometric measurements; for example, HNE absorbs in the UV range at 220 nm, and it can be detected free in samples after separation from other aldehydes by HPLC [65].…”

Section: Analysis Of Free Aldehydic Oxidation Products and Their Metamentioning

confidence: 99%

See 1 more Smart Citation

Chemistry and analysis of HNE and other prominent carbonyl-containing lipid oxidation compounds

Sousa

Pitt

Spickett

2017

Free Radical Biology and Medicine

145

105

View full text Add to dashboard Cite

The process of lipid oxidation generates a diverse array of small aldehydes and carbonyl-containing compounds, which may occur in free form or esterified within phospholipids and cholesterol esters. These aldehydes mostly result from fragmentation of fatty acyl chains following radical oxidation, and the products can be subdivided into alkanals, alkenals (usually D,β-unsaturated), γ-substituted alkenals and bis-aldehydes.Isolevuglandins are non-fragmented di-carbonyl compounds derived from H 2 -isoprostanes, and oxidation of the ω-3-fatty acid docosahexenoic acid yield analogous 22 carbon neuroketals. Non-radical oxidation by hypochlorous acid can generate D-chlorofatty aldehydes from plasmenyl phospholipids. Most of these compounds are reactive and have generally been considered as toxic products of a deleterious process. The reactivity is especially high for the D,β-unsaturated alkenals, such as acrolein and crotonaldehyde, and for γ-substituted alkenals, of which 4-hydroxy-2-nonenal and 4-oxo-2-nonenal are best Page | 2 known. Nevertheless, in recent years several previously neglected aldehydes have been investigated and also found to have significant reactivity and biological effects; notable examples are 4-hydroxy-2-hexenal and 4-hydroxy-dodecadienal. This has led to substantial interest in the biological effects of all of these lipid oxidation products and their roles in disease, including proposals that HNE is a second messenger or signalling molecule.However, it is becoming clear that many of the effects elicited by these compounds relate to their propensity for forming adducts with nucleophilic groups on proteins, DNA and specific phospholipids. This emphasizes the need for good analytical methods, not just for free lipid oxidation products but also for the resulting adducts with biomolecules. The most informative methods are those utilizing HPLC separations and mass spectrometry, although analysis of the wide variety of possible adducts is very challenging. Nevertheless, evidence for the occurrence of lipid-derived aldehyde adducts in biological and clinical samples is building, and offers an exciting area of future research. AbbreviationsAcr-dG, 1,N2-propanodeoxyguanosine; AGEs, advanced glycation end-product; ALEs, advanced lipoxidation end product; ApoB100, apolipoprotein B100; ApoE, apolipoprotein E; ARP, aldehyde reactive probe; AspN, endoproteinase AspN (flavastacin); βLG, β-lactoglobulin; BHZ, biotin hydrazide; BN-PAGE, blue native polyacrylamide gel

show abstract

Section: Quantification Of Aldehydes In Human Plasma or Serummentioning

confidence: 54%

Section: Analysis Of Free Aldehydic Oxidation Products and Their Metamentioning

confidence: 99%

Chemistry and analysis of HNE and other prominent carbonyl-containing lipid oxidation compounds

Sousa

Pitt

Spickett

2017

Free Radical Biology and Medicine

145

105

View full text Add to dashboard Cite

show abstract

“…Combined treatment with α-and β-Naphthoflavone had the most marked effect in on TAC, which significantly increased TAC in comparison to the PC group (P<0.05). MDA is regarded as a typical and sensitive biochemical marker following cell exposure to ROS and its accumulation reflects the extent of OS and indirectly that of cellular antioxidant capacity (25). In the current study, MDA in cells subjected to H 2 O 2 (PC group) increased to ~2.5-fold higher compared with NC cells, reaching 13.24 µmol/mg protein (P<0.05, Fig.…”

Section: Resultsmentioning

confidence: 46%

α- and β-Naphthoflavone synergistically attenuate H2O2-induced neuron SH-SY5Y cell damage

Zhu

et al. 2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. Previous studies have demonstrated an association between neurological diseases and oxidative stress (OS).Naphthoflavone is a synthetic derivative of naturally occurring flavonoids that serves an important role in the treatment and prevention of OS-related diseases. The current study was designed to apply α-and β-Naphthoflavone individually and in combination to counteract the detrimental effects of OS on neurons in vitro. Neuronal SH-SY5Y cells were subjected to 20 µM H 2 O 2 , followed by exposure to 20 µM α-Naphthoflavone and/or 10 µM β-Naphthoflavone. Results indicated that α-and β-Naphthoflavone effectively antagonized the apoptosis-promoting effect of H 2 O 2 on neuronal SH-SY5Y cells, and that β-Naphthoflavone significantly (P<0.05) reversed H 2 O 2 -inhibited cell viability. Notably, co-treatment of α-and β-Naphthoflavone reversed the H 2 O 2 -induced apoptosis rate elevation and cell viability reduction. Further analysis demonstrated that H 2 O 2 inhibited the activities of antioxidant enzymes including catalase, superoxide dismutase and glutathione peroxidase, but this was reversed by the co-treatment with α-and β-Naphthoflavone and selectively enhanced by the treatment with α-or β-Naphthoflavone. H 2 O 2 -stimulated p38 mitogen-activated protein kinase activation was repressed following treatment with α-and/or β-Naphthoflavone, along with a decreased expression of the apoptosis-related factors and inhibited caspase-3 activation. In conclusion, co-treatment with α-and β-Naphthoflavone minimized H 2 O 2 -led neuron damage compared with treatment with α-or β-Naphthoflavone, suggesting a synergetic effect between α-and β-Naphthoflavone. This indicates that utilizing α-and β-Naphthoflavone together in the clinical setting may provide a novel therapeutic for neurological disease.

show abstract

“…11 Various electrochemical, separation and spectroscopic methods were applied for analysis of MDA in various biological matrices. Two more recent review articles 11,12 were summarized the progresses and some draw-backs of MDA analysis in biological samples. 11 Formation of an adduct with thiobarbitoric acid (TBA) and its spectroscopic, liquid chromatographic and electrophoretic determination is the most commonly used analytical procedure for determination of MDA in biological samples.…”

Section: Introductionmentioning

confidence: 99%

Effects of Analytical Procedures on the Repeatability of Malondialdehyde Determinations in Biological Samples

Azizi¹,

Khoubnasabjafari²,

Shahrisa³

et al. 2017

Pharm Sci

View full text Add to dashboard Cite

Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges

Cited by 1,456 publications

References 107 publications

Chemistry and analysis of HNE and other prominent carbonyl-containing lipid oxidation compounds

Chemistry and analysis of HNE and other prominent carbonyl-containing lipid oxidation compounds

α- and β-Naphthoflavone synergistically attenuate H2O2-induced neuron SH-SY5Y cell damage

Effects of Analytical Procedures on the Repeatability of Malondialdehyde Determinations in Biological Samples

Contact Info

Product

Resources

About