Assessment of microRNA‐146a in generalized aggressive periodontitis and its association with disease severity

Abstract

Background and Objective MicroRNA‐146a (miR‐146a) is a small noncoding RNA that plays a critical role in the negative regulation of the innate immune response, and the dysregulation of miR‐146a has been associated with several inflammatory disorders. In generalized aggressive periodontitis (GAgP) the degree of clinical inflammation appears to be similar to that of chronic periodontitis, and, in this situation, age of onset and family history are important additional criteria for diagnosis. This study was performed to evaluate the level of miR‐146a expressed in gingival tissues of patients with GAgP and its association with disease severity. Material and Methods Gingival samples from 18 patients with GAgP and 10 healthy subjects were collected and the level of miR‐146a and its targets, including necrosis factor‐alpha, interleukin‐1beta, and interleukin‐6, were assessed using real‐time PCR. Clinical parameters, including probing depth and clinical attachment loss, were measured and their correlations with the level of miR‐146a were determined. Results Our results demonstrated an elevation in the level of miR‐146a expressed in patients with GAgP compared with healthy controls (P < .001), which was directly associated with disease severity (P < .05). Overexpression of miR‐146a was accompanied by a reduction in the levels of pro‐inflammatory cytokines. Conclusions Our findings suggest that there is an association between miR‐146a and GAgP and imply that miR‐146a may serve as an indicator of periodontal disease severity. However, further studies and additional information are required to confirm this relationship and the precise role of miR‐146a in the development and/or progression of periodontitis.

The Periodontal‐Cardiovascular alliance: Evaluation of miRNA‐146a in subgingival plaque samples of chronic periodontitis patients with and without coronary heart disease

“…In both CP+CHD group and CP group, miRNA levels were positively correlated with BOP, PPD and CAL. This is similar to a recent study where miRNA‐146a levels showed statistically significant associations with PPD and CAL . In vitro studies have shown that expression of miRNA‐146a is upregulated in the presence of inflammatory mediators such as bacterial LPS.…”

“…This is similar to a recent study where miRNA-146a levels showed statistically significant associations with PPD and CAL. 28 In vitro studies have shown that expression of miRNA-146a is upregulated in the presence of inflammatory mediators such as bacterial LPS. Because patients were suffering from periodontitis which is an inflammatory disease, we can assume that this may have a role in higher levels of miRNA-146a with increased disease severity.…”

Salivary Small Extracellular Vesicles Associated miRNAs in Periodontal Status—A Pilot Study

“…It has been reported that hsa-miR-140-5p and hsa-miR-628-5p in gingival crevicular fluid are potential diagnostic biomarkers for periodontitis [25]. Evidence demonstrated hsa-miR-146a-5p might serve as an indicator for periodontal disease severity in both gingival tissues [36] and saliva [37]. However, no report has described miRNA expression in salivary sEVs from healthy, gingivitis and periodontitis patients.…”

Identification of novel key lncRNAs involved in periodontitis by weighted gene co‐expression network analysis

“…Furthermore, miRNAs are thought to be involved in the progression and regulation of inflammatory responses, as several miRNAs (eg, miR‐126, miR‐132 and miR‐221) have been shown to be important regulators of inflammation . Interestingly, a previous study reported an association between miR‐146a and generalized aggressive periodontitis and suggested that miR‐146a might serve as an indicator of periodontal disease severity . Other studies have reported the effects of periodontitis on serum miRNA profiles in rat models and found that the serum levels of several miRNAs (miR‐207, miR‐495 and miR‐376b‐3p) may be valuable biomarkers for periodontitis …”

“…8 Interestingly, a previous study reported an association between miR-146a and generalized aggressive periodontitis and suggested that miR-146a might serve as an indicator of periodontal disease severity. 22,40 Other studies have reported the effects of periodontitis on serum miRNA profiles in rat models and found that the serum levels of several miRNAs (miR-207, miR-495 and miR-376b-3p) may be valuable biomarkers for periodontitis. 41 In summary, this study identified 3 periodontitis-specific ln-cRNAs via WGCNA performed with the Limma package.…”