Assessment of microsatellite instability in urine in the detection of transitional-cell carcinoma of the bladder

Mourah, Samia; Cussenot, Olivier; Vimont, Virginie; Desgrandchamps, François; Teillac, P.; Cochant-Priollet, B.; Duc, A. Le; Fiet, Jean; Soliman, Hany

doi:10.1002/(sici)1097-0215(19981218)79:6<629::aid-ijc13>3.0.co;2-1

Cited by 66 publications

(27 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The primer sequences used for PCR analyses (AB Applied Biosystems, Les Ulis, France) are available from authors upon request. For microsatellite analysis one primer of each marker pair was end‐labeled with 1 of the following fluorophores: 6‐FAM, NED® or HEX (AB Applied Biosystems) PCR was conducted essentially as described previously 15. Briefly, for each microsatellite marker, equal amounts of DNA (20 ng each of constitutional and urine‐extracted) were subjected simultaneously in 2 different PCR tubes to 30–35 cycles of amplification in a Hybaid Touch Down thermocycler (Hybaid, Teddington, UK), as follows: 95°C for 30 sec, followed by the appropriate annealing temperature (52–60°C) for 1 min, an extension step at 72°C for 1 min and a final extension step at 72°C for 5 min.…”

Section: Methodssupporting

confidence: 94%

“…DNA of Family 134702 (CEPH, Paris, France) was used as control. We used a diagnostic set of polymorphic microsatellite markers mapped to chromosomes 4 (D4S243, FGA), 6 (ACTBP2), 9 (D9S162, D9S171, D9S747, IFNA), 14 (MJD52), 16 (D16S310, D16S476), 18 (D18S51, MBP) and 21 (D21S1245) as described previously 15. The primer sequences used for PCR analyses (AB Applied Biosystems, Les Ulis, France) are available from authors upon request.…”

Section: Methodsmentioning

confidence: 99%

“…A non‐invasive diagnostic molecular method based on the detection of LOH or MSI in cells exfoliated in urine, by which 83–95% of bladder carcinomas were detected, has been reported previously 14, 15. The aim of our study was to evaluate the predictive potential of this method in the recurrence detection in a cohort of patients followed‐up after transurethral resection of superficial bladder tumors for up to 48 months.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Non‐invasive molecular detection of bladder cancer recurrence

Amira

Mourah

Rozet³

et al. 2002

Intl Journal of Cancer

Self Cite

View full text Add to dashboard Cite

Transitional cell carcinoma (TCC) is the most common bladder tumor and Ϸ90% of bladder TCC are superficial at initial diagnosis. High recurrence rate and possible progression to muscle invasive disease that is eventually indicated for radical cystectomy are established features of these tumors. Therefore, reliable predictors of tumor recurrence are of critical importance for management of superficial bladder TCC. Successful molecular diagnosis of bladder cancer by detecting genetic lesions: loss of heterozygosity (LOH) or microsatellite instability (MSI) in cells exfoliated in urine has been reported by several groups including ours. The aim of our study was to evaluate the predictive potential of microsatellite analysis of cells exfoliated in urine in the detection of superficial bladder TCC recurrence. We studied 47 Caucasian patients with confirmed superficial bladder TCC (37 pTa, 10 pT1) at initial diagnosis. Blood samples were obtained once from every patient whereas urine samples were collected before each cystoscopy (initial and follow-up). Matched DNAs from blood and urine were subjected to microsatellite analysis in a blinded fashion. The follow-up period ranged 12-48 months after tumor resection. Microsatellite analysis correctly identified 94% (44/47) of primary tumors and 92% (12/13) of tumor recurrences. Interestingly enough, 75% (9/12) of tumor recurrences were molecularly detected 1-9 months before cystoscopic evidence of recurrent disease. This study demonstrated clearly that not only urine microsatellite analysis reliably detected superficial bladder tumors, but also was a reliable test for detecting and predicting tumor recurrence in Caucasian patients. These results warrant multicenter randomized trials.

show abstract

Section: Methodssupporting

confidence: 94%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Non‐invasive molecular detection of bladder cancer recurrence

Amira

Mourah

Rozet³

et al. 2002

Intl Journal of Cancer

Self Cite

View full text Add to dashboard Cite

show abstract

“…This assay was negative in all 10 patients without evidence of cancer (Steiner et al, 1997). Subsequent studies from other investigators have confirmed the reliability of the PCR-based microsatellite analysis of the urine sediments, with 83% sensitivity and 100% specificity (Mourah et al, 1998). These studies support further study of microsatellite analysis of urine sediment DNA for the detection of primary and recurrent bladder cancer.…”

Section: Bladder Cancermentioning

confidence: 57%

Molecular detection approaches for smoking associated tumors

2002

View full text Add to dashboard Cite

Smoking is directly responsible for approximately 90% of lung cancers and is also strongly associated with cancers of the head and neck, esophagus and urinary bladder. Our growing understanding of the molecular changes that underlie cancer progression has contributed to the development of novel molecular approaches for the detection of cancer. In this study, we review a number of recent studies that have used molecular techniques to detect neoplastic DNA from lung, head and neck, esophagus and bladder cancer. The majority of these approaches are based on polymerase chain reaction (PCR) based assays. These PCR-based techniques can detect a few clonal cancer cells containing a specific DNA mutation, microsatellite alteration, or CpG island methylation among an excess background of normal cells. The ability to accurately detect a small number of malignant cells in a wide range of clinical specimens including sputum, saliva, bronchoalveolar lavage fluid, urine, serum, plasma, or tissue has significant implications for screening high-risk individuals (such as cigarette smokers) for cancer.

show abstract

“…Steiner et al [111] correctly diagnosed 20 of 21 patients being followed for BC recurrence using microsatellite analysis with 20 markers in a blinded fashion. Mowah et al [112] reported similar results by detecting microsatellite instability in 10 of 12 patients with BC.…”

Section: Microsatellite Instabilitymentioning

confidence: 67%

Schistosomiasis and Neoplasia

Yosry

2006

Infection and Inflammation: Impacts on Oncogenesis

View full text Add to dashboard Cite

Schistosomiasis is endemic in at least 75 tropical and subtropical countries where 600 million people are at risk of which over 200 million are infected. Three species, S. hematobium, S. mansoni and S. japonicum, account for the majority of human infections. There is sufficient evidence that S. hematobium, the predominant etiologic agent for urinary schistosomiasis, is carcinogenic to humans leading to squamous cell carcinoma of the urinary bladder, a relatively uncommon vesical cancer in nonendemic areas. There is limited evidence suggesting that S. japonicum is possibly carcinogenic to humans leading to colorectal cancer and is a risk factor for hepatocellular carcinoma formation. There is inadequate evidence for the carcinogenicity of S. mansoni in humans. S. mansoni may still be linked to hepatocellular carcinoma through potentiating the effects of hepatitis B virus and hepatitis C virus on the liver. In this article, the relationship between schistosomiasis and neoplasia will be reviewed.

show abstract

Assessment of microsatellite instability in urine in the detection of transitional-cell carcinoma of the bladder

Cited by 66 publications

References 18 publications

Non‐invasive molecular detection of bladder cancer recurrence

Non‐invasive molecular detection of bladder cancer recurrence

Molecular detection approaches for smoking associated tumors

Schistosomiasis and Neoplasia

Contact Info

Product

Resources

About