Assessment of molecular diversity in doubled haploid lines of Camelina (Camelina sativa), as a new emerging oil crop

Abstract

Camelina [Camelina sativa (L.) Crantz], an oilseed crop, belongs to Brassicaceae family. Two unique features of camelina in comparison with the main oil crops are adaptation to different environments and also its unique oil composition. Development of doubled haploid plants is one of the essential methods for the crop improvement. This research was conducted to evaluate the genetic variation of 81 Camelina doubled haploid (DH) lines obtained from fifteen crosses by ISSR markers. The total number of amplified bands was 243, of which 239 bands (98.3%) showed polymorphism. The percentage of polymorphic bands (PPB) varied between 93.75 and 100. The size of the bands ranged from 50 to 1,700 base pairs. The informative ISSRs were identified by estimating marker features: polymorphism information content (PIC), effective multiplex ratio (EMR), marker index (MI) and resolving power (RP). Three markers had higher RP values (9.88, 8.5 and 7.46) and were the most informative markers to identify the DH lines. Cluster analysis based on Complete algorithm divided the lines into five groups, indicating relatively clear configuration from the geographic distribution patterns of the parents of the doubled haploid lines. Principal coordinate analysis (PCoA) classified the 81 camelina DH lines into six groups. The lines grouping by these two methods was similar to each other. The ISSR markers detected high polymorphism to reveal genetic variation of camelina DH lines. The findings of this research, along with biochemical traits, can improve classical and molecular breeding programs of camelina.