Assessment of motility of ejaculated, liquid-stored boar spermatozoa using computerized instruments

Tejerina, Francisco; Buranaamnuay, Kakanang; Saravia, F.; Wallgren, Margareta; Rodriguez‐Martinez, Heriberto

doi:10.1016/j.theriogenology.2008.01.027

Cited by 31 publications

(20 citation statements)

References 28 publications

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“…Moreover, the use of CASA also allows obtaining more objective, detailed and repeatable determination of semen motility characteristics of sperm cells in boar ejaculates (Tejerina et al. ; Contri et al. ).…”

Section: Accurate and Precise Semen Assessment Is Essential In Efficimentioning

confidence: 99%

Efficient Boar Semen Production and Genetic Contribution: The Impact of Low‐Dose Artificial Insemination on Fertility

Broekhuijse

Gaustad

Guillén³

et al. 2015

Reprod Domestic Animals

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Contents Diluting semen from high fertile breeding boars, and by that inseminating many sows, is the core business for artificial insemination (AI) companies worldwide. Knowledge about fertility results is the reason by which an AI company can lower the concentration of a dose. Efficient use of AI boars with high genetic merit by decreasing the number of sperm cells per insemination dose is important to maximize dissemination of the genetic progress made in the breeding nucleus. However, a potential decrease in fertility performance in the field should be weighed against the added value of improved genetics and, in general, is not tolerated in commercial production. This overview provides some important aspects that influence the impact of low‐dose AI on fertility: (i) the importance of monitoring field fertility, (ii) the need for accurate and precise semen assessment, (iii) the parameters that are taken into account, (iv) the application of information from genetic and genomic selection and (v) the optimization when using different AI techniques. Efficient semen production, processing and insemination in combination with increasing use of genetic and genomic applications result in maximum impact of genetic trend.

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Section: Accurate and Precise Semen Assessment Is Essential In Efficimentioning

confidence: 99%

Efficient Boar Semen Production and Genetic Contribution: The Impact of Low‐Dose Artificial Insemination on Fertility

Broekhuijse

Gaustad

Guillén³

et al. 2015

Reprod Domestic Animals

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show abstract

“…An average CoV across 10 samples, each measured 5 times, of 5.2% for total motility and 6.9% for progressive motility suggest a good level of precision for motility analysis results. In an evaluation of two CASA systems using boar semen at a concentration of 40 × 10 6 sperm/ml, one system (QualiSperm ® , Biophos, Geneva, Switzerland) was found to have a similar CoV (6.0%), while another (SM‐CMA ® , Mika Medical GmbH, Rosenheim, Germany) resulted in 12.4% (Tejerina, Buranaamnuay, Saravia, Wallgren, & Rodriguez‐Martinez, ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a portable device for assessment of motility in stallion semen

Buss

Aurich

2018

Reprod Domestic Animals

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Contents In horse breeding, quality assessment of semen before insemination is often requested. Non‐laboratory‐based techniques for objective analysis of sperm motility are thus of interest. The aim of this study was evaluating a portable device for semen analysis (Ongo sperm test) and its comparison with computer‐assisted semen analysis (CASA). Semen was collected from 10 stallions, diluted to 100, 50 and 25 × 106 sperm/ml and analysed for total (TM) and progressive motility (PM). The final sperm concentration influenced total motility analysed by Ongo (p < 0.05) which was higher at 100 × 106 sperm/ml when compared to 25 × 106 sperm/ml (p < 0.05) but not when compared to 50 × 106 sperm/ml (n.s.). Sperm concentration did not influence total motility when assessed by SpermVision (n.s.). Agreement between methods was evaluated by correlation analysis and Bland–Altman plot. Intra‐assay variation of Ongo was 5.2% ± 3.0 for TM and 6.9% ± 3.4 for PM. Correlation between Ongo and CASA was r = 0.79, 0.88 and 0.83 for 100, 50 and 25 × 106 sperm/ml for TM, and r = 0.87, 0.89 and 0.87 for PM, respectively (all p < 0.001). At the 100 and 25 mio/ml dilutions, the difference between the two systems deviated significantly from 0, while no such bias existed at the 50 mio/ml dilution (TM Ongo 85.0%, CASA 82.3%; PM Ongo 64.1%, CASA 66.1%). The 95% confidence interval was 19.9%, 18.9% and 19.2% ± mean for TM and 20.7%, 17.4% and 20.3% ± mean for 100, 50 and 25 × 106 sperm/ml, respectively. In conclusion, Ongo sperm test sperm motility data were strongly correlated with data obtained by CASA. In addition, at a concentration of 50 × 106 sperm/ml values measured with both systems were close to identical. At this concentration, which is recommended in equine AI, Ongo and CASA can be used interchangeably.

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“…However, they assessed sperm motility by subjective visual estimation, and no data showed about sperm kinetics. Tejerina et al (2008) discussed that sperm motility parameters analysed using CASA system provided a more objective assessment of sperm motility in mammals. CASA technology has several advantages as compared to manual counting, such as, fast analysis; increase consistence when the same setting is used, and high statistical power due to objective analysis of numerous sperm (Schleh and Leoni, 2013;Lammers et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

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Saedeghi¹,

Núñez²,

Soler³

et al. 2017

Turk. J. Fish. Aquat. Sci.

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In the present work, the effects of the activation media with different osmolality and storage conditions on sperm motility and kinetic parameters over time after activation, using CASA system (ISAS®) in Danio rerio were analysed. The effect of pooled and individual ejaculate samples was also studied. Spermatozoids activated with high osmolality medium showed higher values in motility parameters and kinematic parameters in comparison with low osmolality medium. Respect to the individual ejaculates or the pool, although significant differences were observed in some parameters, no relevant changes were observed in total motility neither in kinetic parameter. Total motility and velocity of sperm showed significant lower values after sperm storage for 24 h at 4 ºC.

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Assessment of motility of ejaculated, liquid-stored boar spermatozoa using computerized instruments

Cited by 31 publications

References 28 publications

Efficient Boar Semen Production and Genetic Contribution: The Impact of Low‐Dose Artificial Insemination on Fertility

Efficient Boar Semen Production and Genetic Contribution: The Impact of Low‐Dose Artificial Insemination on Fertility

Evaluation of a portable device for assessment of motility in stallion semen

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