Assessment of Mouse Germinal Vesicle Stage Oocyte Quality by Evaluating the Cumulus Layer, Zona Pellucida, and Perivitelline Space

Zhou, Hongxia; Ma, Yuzhen; Liu, Ying-Lei; Ying, Chen; Zhou, Cheng-Jie; Wu, Sudong; Shen, Jiang-Peng; Liang, Cheng-Guang

doi:10.1371/journal.pone.0105812

Cited by 17 publications

(14 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is well known that optimal cumulus cell expansion is essential for normal oocyte maturation, oocyte cytoplasmic maturation in particular [45,46]. Impaired cytoplasmic maturation was associated with poor cumulus cell development [45,47]. The data in this study showed that cumulus cell expansion reached a maximum, with visible corona radiata, after 4RI biphasic IVM.…”

Section: Discussionmentioning

confidence: 51%

Effects of Short-Term Inhibition of Rho Kinase on Dromedary Camel Oocyte In Vitro Maturation

Tukur

Aljumaah

Swelum

et al. 2020

Animals

View full text Add to dashboard Cite

This is the first report on a biphasic in vitro maturation (IVM) approach with a meiotic inhibitor to improve dromedary camel IVM. Spontaneous meiotic resumption poses a major setback for in vitro matured oocytes. The overall objective of this study was to improve in vitro maturation of dromedary camel oocytes using ROCK inhibitor (Y-27632) in a biphasic IVM to prevent spontaneous meiotic resumption. In the first experiment, we cultured immature cumulus–oocyte complexes (COCs, n = 375) in a prematuration medium supplemented with ROCK inhibitor (RI) for 2 h, 4 h, 6 h, and 24 h before submission to normal in vitro maturation to complete 28 h. The control was cultured for 28 h in the absence of RI. In the first phase of experiment two, we cultured COCs (n = 480) in the presence or absence (control) of RI for 2 h, 4 h, 6 h, and 24 h, and conducted real-time relative quantitative PCR (qPCR) on selected mRNA transcripts. The same was done in the second phase, but qPCR was done after completion of normal IVM. Assessment of nuclear maturation showed that pre-IVM for 4 h yielded an increase in MII oocyte (54.67% vs. 26.6% of control; p < 0.05). As expected, the same group showed the highest degree (2) of cumulus expansion. In experiment 2, qPCR results showed significantly higher expression of ACTB and BCL2 in the RI group treated for 4 h when compared with the other groups. However, their relative quantification after biphasic IVM did not reveal any significant difference, except for the positive response of BCL2 and BAX/BCL2 ratio after 4 and 6 h biphasic IVM. In conclusion, RI prevents premature oocyte maturation and gave a significantly positive outcome during the 4 h treatment. This finding is a paradigm for future investigation on dromedary camel biphasic IVM and for improving the outcome of IVM in this species.

show abstract

Section: Discussionmentioning

confidence: 51%

Effects of Short-Term Inhibition of Rho Kinase on Dromedary Camel Oocyte In Vitro Maturation

Tukur

Aljumaah

Swelum

et al. 2020

Animals

View full text Add to dashboard Cite

show abstract

“…The formation of junctional complexes between the oocyte and granulosa cells begins during the transition of primordial follicles to primary-secondary follicles when the zona pellucida (ZP) glycoproteins are secreted and form a matrix between oocyte and granulosa cells 25 . Although a perivitelline space appears between the ZP and the oocyte cell membrane 26,27 , transzonal projections (TZPs) form between the oocyte and granulosa cells and are physiologically and functionally critical for follicular survival 28,29 . One of the components of TZPs is connexon, a hexamer of connexin37 (Gja4), which restricts gap junctional communication transport to factors with a molecular weight lower than 1,000 Da 30 .…”

Section: Discussionmentioning

confidence: 99%

Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles

Kawai

Shimada

2020

Sci Rep

View full text Add to dashboard Cite

the mammalian ovarian follicle is comprised of the germ cell or oocyte surrounded by the somatic cells, the granulosa and theca cells. the ovarian stroma, including the collagen-rich matrix that supports the three-dimensional disk-like follicular structure, impacts the integrity of the ovarian follicle and is essential for follicular development. Maintaining follicular integrity during cryopreservation has remained a limiting factor in preserving ovarian tissues for transplantation because a significant proportion of developed follicles in the frozen-thawed ovaries undergo atresia after transplantation. In this study, we show for the first time that during vitrification of the mouse ovary, the attachment of the oocyte to the granulosa cells was impaired by the loss of the cadherin adhesion molecules. importantly, exposure to a high osmotic solution greatly decreased the ratio of oocyte diameter to the diameter of its follicle but did not alter the collagen-rich matrix surrounding the follicles. By treating ovaries briefly with collagenase before exposure to the hyper-osmotic solution the ratio of oocyte diameter to follicle diameter was maintained, and cadherin adhesion junctions were preserved. When frozen-thawed ovaries were transplanted to the bursa of recipient hosts, pretreatment with collagenase significantly increased serum levels of AMH, the number of intact follicles and the total number of viable offspring compared to frozen-thawed ovaries without collagenase pretreatment, even 6 months after transplantation. Thus, the collagenase pretreatment could provide a beneficial approach for maintaining the functions and viability of cryopreserved ovaries in other species and clinically relevant situations.The fertility of young women with malignancies is dramatically decreased by chemical anti-cancer therapies 1,2 . To preserve patient fertility, assisted reproductive technology (ART) is often utilized. When ART is used in infertile women to obtain an increased number of mature oocytes that will enable the best probability of achieving a live birth, controlled ovarian stimulation (COS) is commonly used 3 . However, gonadotropin treatment can lead to the stimulation of non-physiological levels of estrogens that increase the risk of malignant progression in patients with the luminal type of breast cancer 4 . In the case of childhood cancer patients, most ovarian follicles are only at an early stage of development and lack the ability to respond to gonadotropins to reach the ovulatory stage 5 . Therefore, general ART procedures requiring mature oocytes are not adaptable for fertility preservation in young cancer patients. However, cryopreservation of ovarian tissue containing immature oocytes in preantral follicles is an experimental option for patients before the start of chemical anti-cancer therapy 6 .A slow-freezing technique is commonly used for ovarian cryopreservation 7-9 . Ovarian tissues are treated with cryoprotectants such as DMSO, ethylene glycol or sucrose 10 . The ovarian tissues are cooled and then ...

show abstract

“…ZP thickness in individual preembryos has been proposed to be a marker of normal human preembryo development because a correlation was observed between ZP thickness and implantation of thawed as well as fresh preembryos [26,27]. A thinner portion of the zona pellucida is very likely necessary for appropriate hatching during the blastocyst stage.…”

Section: Discussionmentioning

confidence: 99%

High Serum FSH is Associated with Brown Oocyte Formation and a Lower Pregnacy Rate in Human IVF Parctice

Deng²,

Luo³

et al. 2016

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: To investigate whether brown zona pellucida (ZP) of oocytes affects the outcome of fertilization, embryo quality and pregnancy rate in in vitro fertilization-embryo transfer (IVF-ET). Methods: Based on the ZP color of their oocytes, a total number of 703 patients dated from 2012 to 2014 were divided into a normal egg group (group A) and a brown oocyte group (group B), with 629 and 74 cases, respectively. Clinical characteristics, gonadotropin (Gn) days, Gn dosage, serum hormone levels on the day of human chorionic gonadotropin (HCG) injection, ZP thickness (ZPT) of the eggs, fertilization rate, rescue intracytoplasmic sperm injection (rICSI) rate, good-quality embryo rate and pregnancy rate were compared between the two groups. Results: No significant differences were found in the duration and the causes of infertility, and their basal level of endocrine hormone before IVF-ET between normal egg group and brown egg group. The level of serum hormone including estradiol, progesterone and luteinizing hormone on the day of HCG injection were again similar. Moreover, there were no differences in number of mature oocytes, oocyte fertilization rates and rICSI rates after IVF between the two groups. However, we observed that the ZPT of brown oocytes (group B) was higher than that of normal oocytes (group A). Moreover, the Gn dosage and FSH levels on the day of HCG injection were significantly higher in group B than in group A and the good-quality embryo rate and pregnancy rate in group B were lower than those in group A. Conclusion: Compared with normal eggs, oocytes with a brown ZP were found to have a higher ZPT, lower embryo quality and lower pregnancy rate, which might be due to a high Gn dosage injection and high serum FSH levels during IVT-ET cycles.

show abstract

Assessment of Mouse Germinal Vesicle Stage Oocyte Quality by Evaluating the Cumulus Layer, Zona Pellucida, and Perivitelline Space

Cited by 17 publications

References 53 publications

Effects of Short-Term Inhibition of Rho Kinase on Dromedary Camel Oocyte In Vitro Maturation

Effects of Short-Term Inhibition of Rho Kinase on Dromedary Camel Oocyte In Vitro Maturation

Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles

High Serum FSH is Associated with Brown Oocyte Formation and a Lower Pregnacy Rate in Human IVF Parctice

Contact Info

Product

Resources

About