Assessment of multi-step processes for an integral use of the biomass of the marine microalga Amphidinium carterae

López-Rodríguez, M.; Cerón-García, M.C.; López-Rosales, L.; González-López, Cynthia Victoria; Molina-Miras, A.; Ramírez-González, Abigail; Sánchez-Mirón, A.; Camacho, F. Garcı́a; Grima, E. Molina

doi:10.1016/j.biortech.2019.03.041

Cited by 16 publications

(3 citation statements)

References 21 publications

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“…However, Amphidinium also has other bioactive compounds with commercial interest, like polyunsaturated fatty acids and carotenoids [15]. The species Amphidinium carterae is the most used of the genus in studies for biomass production with photobioreactors for production of carotenoids, fatty acids, and amphidinols [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Effects of the Light Irradiance on the Growth and Lipid Content of Amphidinium carterae (Dinophyceae) for Biofuel Production

2022

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The irradiance level used to the microalgae cultures can modify the growth and proximate composition, however, this response is specie speci c. The dino agellate group had the potential to be used as a source to biofuel production. In this study was evaluated the effect of ve irradiance levels (50, 100, 150, 200, and 250 µmol photon m -2 s -1 ) on the growth rate, proximal composition, pigment content, and photosynthesis of Amphidinium carterae. The highest cell concentration was for the cultures at 150 µmol photon m -2 s -1 (130 × 10 3 cells mL -1 ), and the lowest values for 50 µmol photon m -2 s -1 (49 × 10 3 cells mL -1 ). The cultures maintained under the low irradiance (50 µmol photon m -2 s -1 ) had the highest values of total dry weight (TDW) (13418 pg cell -1 ), organic dry weight (ODW) (3836 pg cell -1 ), and inorganic dry weight (IDW) (9582 pg cell -1 ). The protein content as the general trend increases signi cantly concerning the irradiance level, with the higher values (87.47 pg cell -1 ) at high irradiance (250 µmol photon m -2 s -1 ).Carbohydrate content was different by the effect of irradiance, with the higher values (32.85 pg cell -1 ) at the low irradiance used (50 µmol photon m -2 s -1 ). Lipid content was modi ed by the effect of irradiance, with the highest values (534.74 pg cell -1 ) at the low irradiance used (50 µmol photon m -2 s -1 ). As a general trend, the high irradiances increased the photosynthesis curves. These ndings demonstrate that the strain of A. carterae used in this work can grow in high irradiances (100 to 250 µmol photon m -2 s -1 ) and increase signi cantly the lipid content on low irradiance used (50 µmol photon m -2 s -1 ).

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Section: Introductionmentioning

confidence: 99%

Effects of the Light Irradiance on the Growth and Lipid Content of Amphidinium carterae (Dinophyceae) for Biofuel Production

2022

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“…Amphidinium carterae is a cosmopolitan species that is widely distributed in temperate-to-tropical waters (Ismael et al 1999;Prabowo and Agusti 2019). Amphodinium carterae has become a common species frequently causing HAB outbreaks in recent years; therefore, it has received great interest and considerable attention (Abreu et al 2019;López-Rodríguez et al 2019;Martinez et al 2019;Seto et al 2019). Recently, A. carterae has been isolated from Kauauroa waters, New Zealand (Echigoya et al 2005), North Arabian Sea (Baig et al 2006), Bahamas (Meng et al 2010), the northern Ionian Sea (Pagliara and Caroppo 2012), China sea area (Kong et al 2016), the Gulf of Maine (Seto et al 2019), Florida Bay, and the Florida Keys (Accoroni et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Recombinase-aided amplification combined with lateral flow dipstick for the rapid detection of Amphidinium carterae

Zhang

Liu

et al. 2021

J Appl Phycol

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In the context of global climate change, the frequency and duration of harmful algal blooms (HABs) due to eutrophic coastal waters have increased. HABs can cause marine ecological imbalance, massive socio-economic damage, and potential public health problems. Therefore, accurate and specific techniques that can rapidly detect harmful algae are critical to expedite early monitoring and prediction of HABs. Although some methods based on amplification have previously been described, limitations associated with limited popularity and high costs still exist. In this study, the partial sequence of nuclear ribosomal DNA from Amphinidium carterae was selected as the target region. The biotin-labeled recombinase-aided amplification (RAA) products and the 6-carboxyfluorescein-labeled probe were specifically conjugated, which formed double-labeled RAA products analyzed by lateral flow dipstick (LFD). A rapid RAA-LFD assay aimed at visually detecting A. carterae was established and optimized. The specificity and sensitivity of RAA-LFD were determined. The nucleic acid test with other control algal species yielded negative results, which indicated that RAA-LFD assay can specifically detect A. carterae. The detection limit of RAA-LFD assay for A. carterae was 8.49 ng μL −1 genomic DNA and 1 cell mL −1 . The whole detection process of RAA-LFD could be completed within 50 min. In conclusion, the proposed RAA-LFD assay may be promising for the efficient, specific, and rapid detection of A. carterae in monitoring efforts.

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“…Dinoflagellates contain high levels of omega-3 PUFAs, especially DHA. Their cultivation in suspension, however, suffers from low growth rates and is frequently hampered by their sensitivity to shear stress (Dixon and Syrett 1988;Gibson and Thomas 1995;Gallardo-Rodríguez et al 2009;López-Rosales et al 2015;Wang and Lan 2018), although continuing efforts are being made to address these issues using either open or closed cultivation systems (Fuentes-Grünewald et al 2016;Molina-Miras et al 2018;López-Rodríguez et al 2019). Cell immobilization using a twin-layer porous substrate photobioreactor (TL-PSBR) was recently applied successfully to the peridinin-producing marine dinoflagellate Symbiodinium voratum, which resulted in much higher growth rates compared to a suspension culture (Benstein et al 2014;Langenbach and Melkonian 2019).…”

Section: Introductionmentioning

confidence: 99%

Biofilm cultivation of marine dinoflagellates under different temperatures and nitrogen regimes enhances DHA productivity

et al. 2020

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Dinoflagellates contain large amounts of omega-3 fatty acids, including the nutritionally important docosahexaenoic acid (DHA). However, their cultivation in suspensions is characterized by low growth rates. Twin-layer porous substrate photobioreactors (TL-PSBRs) have been shown to support growth of different microalgal species, including the robust dinoflagellate Symbiodinium voratum. In the present study, the potential of cultivating marine autotrophic dinoflagellate species in a TL-PSBR for DHA production was explored. Based on initial screening experiments, two Symbiodinium species with high biomass and DHA productivities were selected: the symbiotic Symbiodinium microadriaticum CCAC 2475 B and the free-living Symbiodinium voratum CCAC 3869 B. The effects of three different temperatures (17, 22 and 27°C) and nitrogen regimes (nitrate, ammonium and nitrogen-free) on biomass growth, total lipid accumulation and fatty acid methyl esters (FAMEs) content, with emphasis on DHA, were evaluated. The two lower temperatures (17 and 22°C) enhanced growth and total lipid accumulation of S. microadriaticum CCAC 2475 B and S. voratum CCAC 3869 B. Cultivation at 22°C and nitrogen limitation led to a significant positive effect on DHA productivity. Symbiodinium. microadriaticum CCAC 2475 B reached a DHA productivity of 145.4 mg m −2 day −1 and DHA content in the dry biomass of 2% (w/w) after 4 days of nitrogen depletion. The results of the present study demonstrated that autotrophic dinoflagellates, when cultivated on a TL-PSBR, produce comparable amounts of lipids and fatty acids to other commercially used microalgal species including the valuable DHA.

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Assessment of multi-step processes for an integral use of the biomass of the marine microalga Amphidinium carterae

Cited by 16 publications

References 21 publications

Effects of the Light Irradiance on the Growth and Lipid Content of Amphidinium carterae (Dinophyceae) for Biofuel Production

Effects of the Light Irradiance on the Growth and Lipid Content of Amphidinium carterae (Dinophyceae) for Biofuel Production

Recombinase-aided amplification combined with lateral flow dipstick for the rapid detection of Amphidinium carterae

Biofilm cultivation of marine dinoflagellates under different temperatures and nitrogen regimes enhances DHA productivity

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