Assessment of n-alkanes, long-chain fatty alcohols and long-chain fatty acids as diet composition markers: The concentrations of these compounds in rangeland species from Sudan

Ali, H. A. M.; Mayes, R. W.; Hector, B. L.; Ørskov, E. R.

doi:10.1016/j.anifeedsci.2005.02.026

Cited by 40 publications

(53 citation statements)

References 23 publications

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“…The dominance of C 31 in the pasture species was also consistent with previous reports (Ali et al, 2005). This makes it easier to quantify oddchain herbage alkanes more accurately than even-chain alkanes, and hence their role as a diet composition marker appears indispensable.…”

Section: Alkane Concentrationssupporting

confidence: 89%

“…To make effective use of these markers in nutritional studies, however, it is important to document location-specific information on the n-alkane profiles of available herbage species (Ali et al, 2005). This is because environmental conditions and geographical locations could influence the pattern of the cuticular wax profile of plant species growing in different places (Samuels et al, 2008).…”

Section: Alkane Concentrationsmentioning

confidence: 99%

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Evaluation of n-alkanes and their carbon isotope enrichments (δ13C) as diet composition markers

Bezabih

Pellikaan

Tolera

et al. 2011

Animal

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Plant cuticular n-alkanes have been successfully used as markers to estimate diet composition and intake of grazing herbivores. However, additional markers may be required under grazing conditions in botanically diverse vegetation. This study was conducted to describe the n-alkane profiles and the carbon isotope enrichment of n-alkanes of common plant species from the Mid Rift Valley rangelands of Ethiopia, and evaluate their potential use as nutritional markers. A total of 23 plant species were collected and analysed for long-chain n-alkanes ranging from heptacosane to hexatriacontane (C 27 to C 36 ), as well as their carbon isotopic ratio ( 13 C/ 12 C). The analysis was conducted by gas chromatography/combustion isotope ratio mass spectrometry following saponification, extraction and purification. The isotopic composition of the n-alkanes is reported in the delta notation (d 13 C) relative to the Vienna Pee Dee Belemnite standard. The dominant n-alkanes in the species were C 31 (mean 6 s.d., 283 6 246 mg/ kg dry matter) and C 33 (149 6 98 mg/kg dry matter). The carbon isotopic enrichment of the n-alkanes ranged from 219.37% to 237.40%. Principal component analysis was used to examine interspecies differences based on n-alkane profiles and the carbon isotopic enrichments of individual n-alkanes. Large variability among the pasture species was observed. The first three principal components explained most of the interspecies variances. Comparison of the principal component scores using orthogonal procrustes rotation indicated that about 0.84 of the interspecies variances explained by the two types of data sets were independent of each other, suggesting that the use of a combination of the two markers can improve diet composition estimations. It was concluded that, while the n-alkane profile of the pasture species remains a useful marker for use in the study region, the d 13 C values of n-alkanes can provide additional information in discriminating diet components of grazing animals.

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Section: Alkane Concentrationssupporting

confidence: 89%

Section: Alkane Concentrationsmentioning

confidence: 99%

Evaluation of n-alkanes and their carbon isotope enrichments (δ13C) as diet composition markers

Bezabih

Pellikaan

Tolera

et al. 2011

Animal

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“…Previous studies indicated that alcohols had great potential, when combined with alkanes, to estimate the composition of complex diets, and acids also showed substantial between-species differences and high faecal recoveries (Bugalho et al, 2004;Ali et al, 2005b;Dove and Charmley, 2008). However, diet estimation using acids was less accurate (Ali et al, 2005b). Therefore, further indoor validation studies are needed before alcohols and acids can be used routinely in diet composition estimates, particularly in relation to the estimation of faecal recoveries and the evaluation of the accuracy of diet composition estimates (Bugalho et al, 2004;Ali et al, 2004 and2005b).…”

Section: Introductionmentioning

confidence: 99%

“…However, diet estimation using acids was less accurate (Ali et al, 2005b). Therefore, further indoor validation studies are needed before alcohols and acids can be used routinely in diet composition estimates, particularly in relation to the estimation of faecal recoveries and the evaluation of the accuracy of diet composition estimates (Bugalho et al, 2004;Ali et al, 2004 and2005b).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, long-chain alcohols (alcohols) and long-chain fatty acids (acids) have received the most attention (Kelman et al, 2003;Bugalho et al, 2004;Ali et al, 2004Ali et al, , 2005aAli et al, and 2005bDove and Charmley, 2008), as the alcohols and acids can be separated and quantified by an extension of the existing procedure used to analyse alkanes (Ali et al, 2004, Bugalho et al, 2004. Previous studies indicated that alcohols had great potential, when combined with alkanes, to estimate the composition of complex diets, and acids also showed substantial between-species differences and high faecal recoveries (Bugalho et al, 2004;Ali et al, 2005b;Dove and Charmley, 2008). However, diet estimation using acids was less accurate (Ali et al, 2005b).…”

Section: Introductionmentioning

confidence: 99%

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The potential use of long-chain alcohols and fatty acids as diet composition markers: factors influencing faecal recovery rates and diet composition estimates in sheep

Liu

Luo

Zhang

et al. 2009

Animal

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The limitations of the alkane technique in estimating the diet components of herbivores call for the introduction of new diet composition markers. Recently, long-chain alcohols (alcohols) and long-chain fatty acids (acids) have received the most attention and show great potential, when combined with alkanes, to estimate composition of complex diets. In the current study, faecal recoveries of alcohols and acids were determined in sheep in four different live weight groups fed three herbage species, either Leymus chinensis, L. dasystachys or Elymus sibiricum. Analysis of variance (ANOVA) was used to examine the effects of herbage species and live weight of sheep on faecal recoveries of individual alcohols and acids. Further, an indoor experiment with six sheep fed a diet of equal proportions, on dry matter (DM) basis, of three herbages was performed, allowing to assess the accuracy of alcohols and/or acids in combination with alkanes, to estimate diet composition. A one-sample t-test was carried out to test the accuracy of these estimates. Results of the first experiment indicated that the faecal recoveries of alcohols and acids were significantly affected by herbage species ( P , 0.05). While the effects were significant or near significant for the faecal recoveries of some alcohols (C24-ol, C30-ol and C26-ol) ( P < 0.05), no effect of live weight on faecal recoveries of acids was observed ( P . 0.05). Therefore, adjustments based on diet-specific faecal recoveries might improve diet composition estimates. This was illustrated by the results of the second experiment. The diet composition estimated from alcohols or all combinations of alcohols with other marker types, after diet-specific correction of faecal recoveries, did not significantly differ from the actual composition ( P . 0.05). However, using acids as additional markers resulted in poorer diet composition estimates. This study confirmed the utility of alcohols, combined with alkanes, as markers to estimate composition of complex diets. Although corrections based on mean faecal recoveries, average over animals and diets, resulted in some accuracy loss, results were still satisfactory and better than without recovery correction.

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Chemotaxonomic Differentiation of Pinus Species Based on n‐Alkane and Long‐Chain Alcohol Profiles of Needle Cuticular Waxes

Gaspar

Nunes

Rodrigues

et al. 2023

Chemistry & Biodiversity

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n‐Alkane and long‐chain alcohol (LCOH) profiles of needle cuticular waxes of nine provenances of three Pinus species (P. taeda. P. pinaster and P. pinea) were determined and their chemotaxonomic importance was studied. n‐Alkanes concentrations were very low in all Pinus spp. A lack of ability of these compounds to differentiate Pinus spp. and their provenances was observed. LCOH concentrations were much higher, being nonacosan‐10‐ol (10‐C29‐OH) the most abundant one in all Pinus spp. Total LCOH concentrations varied (P<0.001) between Pinus spp. with P. taeda presenting the highest (P<0.05) value and P. pinea the lowest one. Differences in LCOH profiles were large (P<0.001) and allowed separation of Pinus spp. in the Principal Component Analysis (PCA). Although a noticeable separation of individuals was not observed. cluster analysis on LCOH concentrations allowed a clear distinction between species, indicating their potential to be used as chemotaxonomic markers to differentiate provenances of different Pinus spp.

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Assessment of n-alkanes, long-chain fatty alcohols and long-chain fatty acids as diet composition markers: The concentrations of these compounds in rangeland species from Sudan

Cited by 40 publications

References 23 publications

Evaluation of n-alkanes and their carbon isotope enrichments (δ13C) as diet composition markers

Evaluation of n-alkanes and their carbon isotope enrichments (δ13C) as diet composition markers

The potential use of long-chain alcohols and fatty acids as diet composition markers: factors influencing faecal recovery rates and diet composition estimates in sheep

Chemotaxonomic Differentiation of Pinus Species Based on n‐Alkane and Long‐Chain Alcohol Profiles of Needle Cuticular Waxes

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