This study aimed to evaluate factors associated with employee motivation and retention in the emergency medical service (EMS) system. MATERIALS AND METHODS: All of the participants were enrolled from 6 EMS centers. Participants were healthcare providers aged 20-59 years old working in an emergency department. The reliability of employee motivation and retention in the EMS system questionnaire had a Cronbach's alpha of 0.93. RESULTS: A total of 141 participants were recruited to participate in this study. The average age was 34.75 ± 7.67 years old. 69.5% were male, and 30.5% were female, respectively. 80.1% of participants worked at the emergency department for less than 10 years, and 22.7% had health problems. Age, marital status, educations, occupation, working hours per week, health problems, and medical scheme were associated with employee motivation and retention in the EMS system. CONCLUSION: Age, marital status, educations, occupations, working hours per week, health problems, and medical schemes were related to employee motivation and retention in the EMS system.