The study aim was to evaluate the antioxidant and antimicrobial activity of Muntingia calabura fruits extracts, cultivated in Colombia. The antioxidant activities of the extracts were also examined using diphenylpicrylhydrazyl (DPPH) method. The antibacterial activity of the extracts was evaluated through disc diffusion method. M. calabura fruits extracts were tested against foodborne pathogens such as Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, and Escherichia coli. Based on the minimal inhibitory concentration (MIC), the highest antimicrobial activity was found against S. aureus and B. cereus in comparison with P. aeruginosa and E. coli. It means that the inhibition degrees are different between Gram positive and Gram negative bacteria. With respect to the antioxidant activity, high values (IC50 = 83.17 ± 1.8) were obtained. These findings suggest the possibility of use of M. calabura for the active films development for the 882 Omar Fernando Cuadro Mogolln et al. food matrixes conservation. This study reports firstly a high degree of antibacterial and antioxidant activities of M. calabura fruit cultivated in Colombia against foodborne pathogens.