Introduction: Adequate nutrition knowledge and dietary intake are important for enhancing athletic performance. Athletes are more likely to be tired and perform poorly during sports when they do not get enough diet. To the best of our knowledge, this study is first of its kind to measure the association of nutritional knowledge, practice, supplement use, and nutrient intake with athletic performance among Nepalese Taekwondo (TKD) players.Methodology:We conducted an analytical cross-sectional study among 293 Taekwondo players of Kathmandu Metropolitan City, between August 2019 and January 2020. A two-stage cluster random sampling technique was used to select the desired number of respondents for the study. Face-to-face interviews were carried out using semi-structured questionnaires. Nutrition knowledge (NK), nutrition practice (NP), supplement use, nutrient intake using 24-hour dietary recall, and anthropometric measurements were taken. A handgrip dynamometer was used to assess the handgrip strength (HGS), as an indirect measure of athletic performance. Univariate and bivariate analyses were employed to find out the association of predictor and outcome variables. Data were analyzed using STATA/MP version 14.1.Results: Out of 293 participants, more than half (56.31% and 55.63%) had poor nutrition knowledge and nutrition practice scores respectively. Total energy intake (2368 Kcal) and protein (13.28%), fat (15.85%), calcium (416 mg), and iron (7 mg) respectively were significantly lower than corresponding Recommended Dietary Allowances (RDA) except carbohydrates intake (71.41%). Nutritional knowledge (r = 0.117), height (r = 0.538), weight (r = 0.651), BMI (r = 0.347), fat (r = 0.075), and energy (0.127) intake showed significant positive correlation with handgrip strength of athletes. The athletic performance were positively associated with training hours per day (β = 0.41, 95% CI: 0.09–0.91), BMI (β = 0.35, 95% CI: 0.09–0.61), NK score (β = 0.13, 95% CI: 0.01–0.25), and energy intake (β = 0.13, 95% CI: 0.12 = 0.14).Conclusions: The results suggested that nutritional knowledge and nutrient intake both were poor among TKD athletes. Height, weight, BMI, nutritional knowledge, energy, and fat intake were positive correlation with athletic performance. Further well-constructed longitudinal research is warranted to identify the robust relationship between nutritional knowledge, practice, different supplement use, and nutrient intake among athletes.