Assessment of osteogenesis for 3D-printed polycaprolactone/hydroxyapatite composite scaffold with enhanced exposure of hydroxyapatite using rat calvarial defect model

Cho, Young‐Sam; Quan, Meiling; Lee, Se-Hwan; Hong, Myoung Wha; Kim, Young‐Yul

doi:10.1016/j.compscitech.2019.107844

Cited by 54 publications

(26 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alternatively, enhancing the exposure of HA to the surface of the scaffold could potentially improve the ion exchange dynamics between scaffolds and medium and, therefore, improve the osteogenic potential of the nHA scaffolds. To do this, ME-AM scaffolds surface erosion using NaOH, [77, 80, 81] as well as bare polymeric scaffolds coating with HA by ultrasonication, [82] or pre-calcification by immersion in an SBF solution, [83] have been proposed. While these studies commonly suggest the correlation of osteogenic genes upregulation or increased matrix mineralization to the enhanced HA exposure, we believe that careful investigations are still required, as using such surface optimization methods have also shown to dramatically change the surface roughness of the scaffolds, making it hard to decouple the effect of HA bioactivity and surface roughness, as the latter is known to affect osteogenic differentiation significantly.…”

Section: Discussionmentioning

confidence: 99%

Effect of Highly Loaded Nanohydroxyapatite Composite Scaffolds Prepared via Melt Extrusion Additive Manufacturing on the Osteogenic Differentiation of Human Mesenchymal Stromal Cells

Cámara-Torres

Sinha

Sánchez³

et al. 2021

Preprint

View full text Add to dashboard Cite

The field of bone tissue engineering seeks to mimic the bone extracellular matrix composition, balancing the organic and inorganic components. In this regard, additive manufacturing (AM) of highly loaded polymer-calcium phosphate (CaP) composites holds great promise towards the design of bioactive scaffolds. Yet, the biological performance of such scaffolds is still poorly characterized. In this study, melt extrusion AM (ME-AM) was used to fabricate poly(ethylene oxide terephthalate)/poly(butylene terephthalate) (PEOT/PBT)-nanohydroxyapatite (nHA) scaffolds with up to 45 wt% nHA, which presented significantly enhanced compressive mechanical properties, to evaluate their in vitro osteogenic potential as a function of nHA content. While osteogenic gene upregulation and matrix mineralization were observed on all scaffold types when cultured in osteogenic media, human mesenchymal stromal cells did not present an explicitly clear osteogenic phenotype, within the evaluated timeframe, in basic media cultures (i.e. without osteogenic factors). Yet, due to the adsorption of calcium and inorganic phosphate ions from cell culture media and simulated body fluid, the formation of a CaP layer was observed on PEOT/PBT-nHA 45 wt% scaffolds, which is hypothesized to account for their osteoinductivity in the long term in vitro, and osteoconductivity in vivo.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Highly Loaded Nanohydroxyapatite Composite Scaffolds Prepared via Melt Extrusion Additive Manufacturing on the Osteogenic Differentiation of Human Mesenchymal Stromal Cells

Cámara-Torres

Sinha

Sánchez³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…As an illustration, 3D-printed HAp ceramics obtained by digital light processing showed the ability to improve the growth and proliferation of MC3 T3-E1 osteoblast precursor cell line as well as facilitate the cell adhesion and migration in vitro (Zeng et al, 2018). Furthermore, in vivo experimental studies have proven that 3Dprinted scaffolds made of HAp and its composites could act as excellent bone replacements, thanks to proper osteoconductivity (Cho et al, 2019b;Sha et al, 2019). It should be mentioned that 3D-printed HAp scaffolds are also found as competent vehicles to deliver osteogenic growth factors like bone morphogenetic protein-2 for improving in vivo bone regeneration (Chen G. et al, 2019).…”

Section: Potential and Significance In Hard Tissue Regeneration And Dmentioning

confidence: 99%

Additive Manufacturing Methods for Producing Hydroxyapatite and Hydroxyapatite-Based Composite Scaffolds: A Review

et al. 2019

View full text Add to dashboard Cite

Hydroxyapatite (HAp) has been considered for decades an ideal biomaterial for bone repair due to its compositional and crystallographic similarity to bioapatites in hard tissues. However, fabrication of porous HAp acting as a template (scaffold) for supporting bone regeneration and growth has been a challenge to biomaterials scientists. The introduction of additive manufacturing technologies, which provide the advantages of a relatively fast, precise, controllable, and potentially scalable fabrication process, has opened new horizons in the field of bioceramic scaffolds. This review focuses on three-dimensional-printed HAp scaffolds and related composite systems, where the calcium phosphate phase is combined with other ceramics or polymers improving the mechanical properties and/or imparting special extra-functionalities. The main applications of three-dimensional-printed HAp scaffolds in bone tissue engineering are presented and discussed; furthermore, this review also emphasizes the most recent achievements toward the development and testing of multifunctional HAp-based systems combining multiple properties for advanced therapy (e.g., bone regeneration, antibacterial effect, angiogenesis, and cancer treatment).

show abstract

“…A scaffold should be biocompatible and biodegradable, and provide suitable mechanical properties, pore sizes, and well-interconnected pores [ 7 , 8 , 9 ]. Many studies using 3D-printing techniques, including stereolithography apparatus (SLA) [ 10 ], selective laser sintering (SLS) [ 11 ], and melting extrusion (3D plotting) [ 12 ], have been proposed for fabricating a scaffold satisfying the abovementioned requirements. The 3D-printing technique is known to enable customized 3D scaffolds with suitable pore size, porosity, and interconnected pore networks using biocompatible and biodegradable materials.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Polycaprolactone/Nano Hydroxyapatite (PCL/nHA) 3D Scaffold with Enhanced In Vitro Cell Response via Design for Additive Manufacturing (DfAM)

Cho

Gwak

2021

Polymers

Self Cite

View full text Add to dashboard Cite

In this study, we investigated the dual-pore kagome-structure design of a 3D-printed scaffold with enhanced in vitro cell response and compared the mechanical properties with 3D-printed scaffolds with conventional or offset patterns. The compressive modulus of the 3D-printed scaffold with the proposed design was found to resemble that of the 3D-printed scaffold with a conventional pattern at similar pore sizes despite higher porosity. Furthermore, the compressive modulus of the proposed scaffold surpassed that of the 3D-printed scaffold with conventional and offset patterns at similar porosities owing to the structural characteristics of the kagome structure. Regarding the in vitro cell response, cell adhesion, cell growth, and ALP concentration of the proposed scaffold for 14 days was superior to those of the control group scaffolds. Consequently, we found that the mechanical properties and in vitro cell response of the 3D-printed scaffold could be improved by kagome and dual-pore structures through DfAM. Moreover, we revealed that the dual-pore structure is effective for the in vitro cell response compared to the structures possessing conventional and offset patterns.

show abstract

Assessment of osteogenesis for 3D-printed polycaprolactone/hydroxyapatite composite scaffold with enhanced exposure of hydroxyapatite using rat calvarial defect model

Cited by 54 publications

References 37 publications

Effect of Highly Loaded Nanohydroxyapatite Composite Scaffolds Prepared via Melt Extrusion Additive Manufacturing on the Osteogenic Differentiation of Human Mesenchymal Stromal Cells

Effect of Highly Loaded Nanohydroxyapatite Composite Scaffolds Prepared via Melt Extrusion Additive Manufacturing on the Osteogenic Differentiation of Human Mesenchymal Stromal Cells

Additive Manufacturing Methods for Producing Hydroxyapatite and Hydroxyapatite-Based Composite Scaffolds: A Review

Fabrication of Polycaprolactone/Nano Hydroxyapatite (PCL/nHA) 3D Scaffold with Enhanced In Vitro Cell Response via Design for Additive Manufacturing (DfAM)

Contact Info

Product

Resources

About