Assessment of oxidative status markers and NO bioavailability in hypertensive disorders of pregnancy

Gomes, Helen Fernanda Barros; Palei, Ana C.; Machado, Jackeline de Souza Rangel; Silva, L M da; Montenegro, Marcelo F.; Jordão, Alceu Afonso; Duarte, Geraldo; Tanus‐Santos, José Eduardo; Cavalli, Ricardo de Carvalho; Sandrim, Valéria C.

doi:10.1038/jhh.2012.58

Cited by 18 publications

(9 citation statements)

References 35 publications

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“…Previous studies of oxidative stress markers during pregnancy or at the time of delivery have used comparable measures such as lipid hydroperoxide and malondialdehyde concentration (Morris et al, 1998), total antioxidant capacity or biological antioxidant potential (Hsieh et al, 2012; Gomes et al, 2013; Watanabe et al, 2013). There is a substantial body of evidence showing that PE is a state of elevated placental oxidative stress (Burton and Jauniaux, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…As the pathology of endothelial dysfunction is a shared characteristic of the hypertensive disorders of pregnancy, it is not entirely surprising that a general measure of lipid peroxidation does not distinguish between the differing clinical states. Lipid peroxidation values begin to fall within 24 h of delivery (Little and Gladen, 1999; Gomes et al, 2013). However, the fact that lipid peroxidation appears still to be higher, at 6 week postpartum, in women who had essential hypertension, as compared to those who had normotensive pregnancies, suggests that a degree of underlying damage may have been present before pregnancy in these women.…”

Section: Discussionmentioning

confidence: 99%

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Oxidative stress markers in hypertensive states of pregnancy: preterm and term disease

et al. 2014

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Discussion continues as to whether de novo hypertension in pregnancy with significant proteinuria (pre-eclampsia; PE) and non-proteinuric new hypertension (gestational hypertension; GH) are parts of the same disease spectrum or represent different conditions. Non-pregnant hypertension, pregnancy and PE are all associated with oxidative stress. We have established a 6 weeks postpartum clinic for women who experienced a hypertensive pregnancy. We hypothesized that PE and GH could be distinguished by markers of oxidative stress; thiobarbituric acid reactive substances (TBARS) and antioxidants (ferric ion reducing ability of plasma; FRAP). Since the severity of PE and GH is greater pre-term, we also compared pre-term and term disease. Fifty-eight women had term PE, 23 pre-term PE, 60 had term GH and 6 pre-term GH, 11 pre-existing (essential) hypertension (EH) without PE. Limited data were available from normotensive pregnancies (n = 7) and non-pregnant controls (n = 14). There were no differences in postpartum TBARS or FRAP between hypertensive states; TBARS (P = 0.001) and FRAP (P = 0.009) were lower in plasma of non-pregnant controls compared to recently-pregnant women. Interestingly FRAP was higher in preterm than term GH (P = 0.013). In PE and GH, TBARS correlated with low density lipoprotein (LDL)-cholesterol (P = 0.036); this association strengthened with inclusion of EH (P = 0.011). The 10 year Framingham index for cardiovascular risk was positively associated with TBARS (P = 0.003). Oxidative stress profiles do not differ between hypertensive states but appear to distinguish between recently-pregnant and non-pregnant states. This suggests that pregnancy may alter vascular integrity with changes remaining 6 weeks postpartum. LDL-cholesterol is a known determinant of oxidative stress in cardiovascular disease and we have shown this association to be present in hypertensive pregnancy further emphasizing that such a pregnancy may be revealing a pre-existing cardiovascular risk.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Oxidative stress markers in hypertensive states of pregnancy: preterm and term disease

et al. 2014

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“…A major feature of hypertensive disorders includes oxidative stress [17] , which impairs NO bioavailability and is associated with lower nitrite levels as compared to normotensive controls [18–20] . While antihypertensive effects of sodium nitrite have been demonstrated in some animal models of hypertension [11,15,16,19–24] , no previous study has addressed the possible antihypertensive effects of oral sodium nitrite in the deoxycorticosterone-salt (DOCA-salt) hypertension model.…”

Section: Introductionmentioning

confidence: 99%

Consistent antioxidant and antihypertensive effects of oral sodium nitrite in DOCA-salt hypertension

et al. 2015

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Hypertension is a common disease that includes oxidative stress as a major feature, and oxidative stress impairs physiological nitric oxide (NO) activity promoting cardiovascular pathophysiological mechanisms. While inorganic nitrite and nitrate are now recognized as relevant sources of NO after their bioactivation by enzymatic and non-enzymatic pathways, thus lowering blood pressure, mounting evidence suggests that sodium nitrite also exerts antioxidant effects. Here we show for the first time that sodium nitrite exerts consistent systemic and vascular antioxidant and antihypertensive effects in the deoxycorticosterone-salt (DOCA-salt) hypertension model. This is particularly important because increased oxidative stress plays a major role in the DOCA-salt hypertension model, which is less dependent on activation of the renin-angiotensin system than other hypertension models. Indeed, antihypertensive effects of oral nitrite were associated with increased plasma nitrite and nitrate concentrations, and completely blunted hypertension-induced increases in plasma 8-isoprostane and lipid peroxide levels, in vascular reactive oxygen species, in vascular NADPH oxidase activity, and in vascular xanthine oxidoreductase activity. Together, these findings provide evidence that the oral administration of sodium nitrite consistently decreases the blood pressure in association with major antioxidant effects in experimental hypertension.

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“…Our group has already shown this antioxidant compensatory mechanism observed in hypertensive disorders of pregnancy, which matches this new finding. The NOx levels remain reduced in supernatants from cells incubated with PE to NT, also suggesting that other mechanisms may be involved in the NO bioavailability [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Glibenclamide Increases Nitric Oxide Levels and Decreases Oxidative Stress in an In Vitro Model of Preeclampsia

et al. 2022

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(1) Background: The bioavailability of nitric oxide (NO) and oxidative stress are important events related to the pathophysiology of preeclampsia (PE). In this present study, we aimed to evaluate the antioxidant effect of glibenclamide (GB) on the NO synthesis, oxidative stress, and antioxidant capacity in endothelial cells incubated with plasma from preeclamptic (PE) and normotensive pregnant women (NT). (2) Methods: Human umbilical vein endothelial cells (HUVECs) were incubated with a plasma pool from 10 NT and 10 PE pregnant women; NO/NOx quantification and ROS levels were assessed by a fluorescence compound; lipid peroxidation was evaluated employing thiobarbituric acid (TBA); and total antioxidant capacity was measured by ferric reduction ability power (FRAP) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). (3) Results: We found that endothelial cells incubated with plasma from PE showed lower NO and NOx levels compared with the NT group. However, GB treatment increased these levels, as well as the antioxidant capacity. Furthermore, a decrease was observed in ROS generation and lipid peroxidation (4) Conclusions: The GB treatment exerted a positive effect on the NO/NOx production by HUVEC incubated with plasma from NT and PE pregnant women, as well as in the reduction in oxidative stress and increase in the antioxidant capacity.

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Assessment of oxidative status markers and NO bioavailability in hypertensive disorders of pregnancy

Cited by 18 publications

References 35 publications

Oxidative stress markers in hypertensive states of pregnancy: preterm and term disease

Oxidative stress markers in hypertensive states of pregnancy: preterm and term disease

Consistent antioxidant and antihypertensive effects of oral sodium nitrite in DOCA-salt hypertension

Glibenclamide Increases Nitric Oxide Levels and Decreases Oxidative Stress in an In Vitro Model of Preeclampsia

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