Assessment of Oxidative Stress Biomarkers in Rat Blood

Effect of transgenerational diabetes via maternal lineage in female rats

“…The procedures used for oxidative stress parameters followed the methodology described by Sinzato et al (2023) [ 17 ]. For this, all biomarkers were estimated in washed erythrocytes.…”

“…This procedure was repeated three times, and then the washed erythrocyte samples were divided into two aliquots: an aliquot was diluted (1:20) into purified water for the measurement of TBARS and –SH levels and another aliquot was diluted into stabilizing solution of 2.7 mM EDTA and 0.7 mM 2-mercaptoethanol (1:20) for H 2 O 2 , SOD, GSH-Px, and CAT measurements. The hemoglobin (Hb) concentration was determined using Drabkin's solution [ 17 ].…”