Assessment of pain‐related behavior and pro‐inflammatory cytokine levels in the rat rotator cuff tear model

Yamazaki, Hironori; Ochiai, Nobuyasu; Kenmoku, Tomonori; Ohtori, Seiji; Sasho, Takahisa; Miyagi, Masayuki; Ishikawa, Tetsuhiro; Kamoda, Hiroto; Orita, Sumihisa; Sasaki, Yuu; Yamaguchi, Takeshi; Kijima, Takehiro; Takahashi, Kazuhisa

doi:10.1002/jor.22486

Cited by 25 publications

(22 citation statements)

References 22 publications

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“…Second, hip pain was suggested by immunohistochemical determination of CGRP expression. Further study is needed to determine pain-related behavior using other methods such as evaluation of weight bearing in walking animals [32][33]. Third, dose dependency could not be found, since only NGF50 and NGF100 groups were documented.…”

Section: Discussionmentioning

confidence: 91%

A novel rat model of hip pain by intra-articular injection of nerve growth factor-characteristics of sensory innervation and inflammatory arthritis

Omae

Nakamura

Ohtori

et al. 2015

Modern Rheumatology

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Section: Discussionmentioning

confidence: 91%

A novel rat model of hip pain by intra-articular injection of nerve growth factor-characteristics of sensory innervation and inflammatory arthritis

Omae

Nakamura

Ohtori

et al. 2015

Modern Rheumatology

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“…Pain‐related behavior is useful to evaluate pain in animal models. Gait disturbance is widely accepted as a pain‐related behavior . However, gait disturbance has not been examined in a rat model of hip OA induced by MIA.…”

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confidence: 99%

Pain-related behavior and the characteristics of dorsal-root ganglia in a rat model of hip osteoarthritis induced by mono-iodoacetate

et al. 2017

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The principal aim of this study was to clarify the time course of pain-related behavior and pain-related sensory innervation in a rat model of hip osteoarthritis (OA) induced by intra-articular injection of mono-iodoacetate (MIA). Using 6-week-old male Sprague Dawley rats, 25 μl of sterile saline of 1% Fluoro-Gold solution (FG) (control group; n = 30) and 25 μl of sterile saline of 1% FG with 2 mg of MIA (MIA group; n = 30) was injected into the right hip joints. Gait function was evaluated using a CatWalk system after 7, 14, 28, 42, and 56 days (n = 5, respectively). Neurons in the dorsal root ganglion (DRG) between L1 and L5 were immunostained for calcitonin gene-related peptide (CGRP) and activating transcription factor-3 (ATF3). Gait analysis revealed the mean six parameters of hind paws at all time points were significantly lower in the MIA group (p = 0.05). The number of CGRP-immunoreactive (-IR) DRG neurons was significantly increased on days 7, 14, 28, and 42 peaking at 14 days in the MIA group. By contrast, expression of ATF3-IR in FG-labeled DRG neurons was significantly increased on days 42 and 57. The FG-labeled DRG neurons were distributed between L1 and L5, mainly at the L4 level. Pain-related behavior indicated by gait disturbance was observed in a MIA model of hip OA in rat. Early elevation of CGRP expression and late expression of ATF-3 were demonstrated in DRG neurons, possibly reflecting inflammatory pain and neuropathic pain in hip OA. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1424-1430, 2017.

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“…Therefore, rats were considered to be a suitable animal for the development of a RCT experimental model. However, few studies have evaluated pain relief in an animal RCT model, and there are currently few systems capable of quantifying rat forelimb function …”

Section: Discussionmentioning

confidence: 99%

“…Yamazaki et al reported that rotator cuff repair not only improved shoulder function, but also reduced inflammatory cytokines. In addition, they also showed that suppression of inflammation improved shoulder function.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of hyaluronic acid or steroid injections for the treatment of a rat model of rotator cuff injury

Yamaguchi

Ochiai

Sasaki

et al. 2015

Journal Orthopaedic Research

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This study evaluated dorsal root ganglia from C3-C7, analyzed gait, and compared the expression of calcitonin generelated peptide (CGRP) which was a marker of inflammatory pain in a rat rotator cuff tear model in which the supraspinatus and infraspinatus tendons were detached; comparisons were made to a sham group in which only the tendons were exposed. Fluorogold was injected into the glenohumeral joint 21 days after surgery in both groups, and saline, steroids, or hyaluronic acid was injected into the glenohumeral joint in the rotator cuff tear group 26 days after surgery. The proportions of CGRP-immunoreactive neurons were higher and the gait parameters were impaired in the rotator cuff tear group compared to in the sham group. However, the CGRP expression was reduced and the gait was improved with steroid or hyaluronic acid injection compared to saline, suggesting that both hyaluronic acid and steroid injections suppressed of inflammation which thought to be provided pain relief. While there were no significant differences, the suppression of CGRP expression and the improved gait after hyaluronic acid and steroid injections suggested that both methods were effective for rat rotator cuff tear model. Keywords: rotator cuff tear model; pain relief; hyaluronic acid; dorsal root ganglion; CatWalkThe main symptoms of a rotator cuff tear (RCT) are shoulder pain and limited range of motion. Conservative treatment for RCT consists of medication, fomentation, or injections into the subacromial bursa (SAB) or glenohumeral joint (GHJ). In addition, physical rehabilitation has also been used. Although both steroid 1-3 and hyaluronic acid (HA) injections have been commonly used as a conservative treatment for RCT, it remains unknown which method is more effective for pain relief 2,4-6 Moreover, repeated steroid injections may result in local tissue degradation, including tendon tearing and steroid arthropathy. 1 Although the cause of pain in shoulder pathology has not been fully clarified, calcitonin gene-related peptide (CGRP) was found to be highly expressed in both the GHJ and SAB in a rat contracture shoulder model. These results indicate that up-regulation of CGRP might be closely related to the cause of pain in frozen shoulder; 7 the same mechanism of up-regulation of CGRP has also been hypothesized to be related to RCT of inflammatory pain. For both the GHJ and SAB, sensory information is transmitted from free nerve endings through the dorsal root ganglia (DRG) at C3-C7 to the spinal cord and brain. 8 For investigation of inflammation-related pain, evaluation at the level of the DRG is important especially the expression of CGRP.The purpose of the present study was (i) to evaluate DRG neurons innervating the GHJ and SAB using immunohistochemistry for CGRP in a rat RCT model and (ii) to determine whether steroid or HA injection would provide more effective in rotator cuff tear model. MATERIALS AND METHODSAll animal protocols were approved by the Ethics Committee of Chiba University and conducted in accorda...

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Assessment of pain‐related behavior and pro‐inflammatory cytokine levels in the rat rotator cuff tear model

Cited by 25 publications

References 22 publications

A novel rat model of hip pain by intra-articular injection of nerve growth factor-characteristics of sensory innervation and inflammatory arthritis

A novel rat model of hip pain by intra-articular injection of nerve growth factor-characteristics of sensory innervation and inflammatory arthritis

Pain-related behavior and the characteristics of dorsal-root ganglia in a rat model of hip osteoarthritis induced by mono-iodoacetate

Efficacy of hyaluronic acid or steroid injections for the treatment of a rat model of rotator cuff injury

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