“…LSCIs have been widely used in various clinical applications, such as diagnosis of keloids classification, [5][6][7][8][9] burn evaluation, [10][11][12][13] observation of microcirculation following treatment, [14][15][16][17] robot-assisted surgery, 18 intraoperative cerebral blood flow monitoring, [19][20][21] surgery monitoring, [22][23][24][25] reperfusion evaluation after tissue transplantation, 26,27 prediction of tissue necrosis risk, [28][29][30] and reperfusion evaluation after oral wound healing. 31,32 To ensure accurate measurement in the region of interest, the imaging region of interest (IROI) must be matched with the LSCI displayed on the monitor screen, which may be time-consuming due to potential spatial mismatching between the IROI and the monitor screen. To address the issue, previous studies have tried aligning the LSCI with a white light image on a monitor screen 19,33 or using a projector to overlay an LSCI on a surgical microscope.…”