Assessment of pesticide induced inhibition of Apis mellifera (honeybee) acetylcholinesterase by means of N-doped carbon dots/BSA nanocomposite modified electrochemical biosensor

Bilal, Sehrish; Sami, Amtul Jamil; Hayat, Akhtar; Rehman, Muhammad Fayyaz ur

doi:10.1016/j.bioelechem.2021.107999

Cited by 26 publications

(13 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Molecular Dynamics (MD) were performed using AMBER14 as a force field in a 20 Å water-filled space around the protein-ligand complexes obtained from molecular docking. The same condition was used as described earlier [61,63,64] and 100 ns MD simulations were obtained. Prism GraphPad ver 7.0 [65] was used to analyze the data.…”

Section: Molecular Docking and Molecular Dynamic Simulationsmentioning

confidence: 99%

2,3-Dihydroquinazolin-4(1H)-one as a New Class of Anti-Leishmanial Agents: A Combined Experimental and Computational Study

et al. 2021

View full text Add to dashboard Cite

Leishmaniasis is a neglected parasitic disease caused by various Leishmania species. The discovery of new protozoa drugs makes it easier to treat the disease; but, conventional clinical issues like drug resistance, cumulative toxicity, and target selectivity are also getting attention. So, there is always a need for new therapeutics to treat Leishmaniasis. Here, we have reported 2,3-dihydroquinazolin-4(1H)-one derivative as a new class of anti-leishmanial agents. Two derivatives, 3a (6,8-dinitro-2,2-disubstituted-2,3-dihydroquinazolin-4(1H)-ones) and 3b (2-(4-chloro-3-nitro-phenyl)-2-methyl-6,8-dinitro-2,3-dihydro-1H-quinazolin-4-one) were prepared that show promising in silico anti-leishmanial activities. Molecular docking was performed against the Leishmanial key proteins including Pyridoxal Kinase and Trypanothione Reductase. The stability of the ligand-protein complexes was further studied by 100 ns MD simulations and MM/PBSA calculations for both compounds. 3b has been shown to be a better anti-leishmanial candidate. In vitro studies also agree with the in-silico results where IC50 for 3a and 3b was 1.61 and 0.05 µg/mL, respectively.

show abstract

Section: Molecular Docking and Molecular Dynamic Simulationsmentioning

confidence: 99%

2,3-Dihydroquinazolin-4(1H)-one as a New Class of Anti-Leishmanial Agents: A Combined Experimental and Computational Study

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Carbon dots have been used to detect several metal ions such as Fe 3+ [13,14,24,[36][37][38][39][40][41][42][43], Hg 2+ [13,16,24,38,40,41,44], Cu 2+ [13, 16, 24, 38-41, 43, 45, 46], Ag + [13,39,40,43], Pb 2+ [14,38,39,40,43], Cr 6+ [13,16,24,41,47] and so on, relative humidity [13,48,49,50], temperature [37], molecules in liquid phase [13,23,51,52], gas molecules, etc. Fluorescence sensors [42,47,53,54], electrochemical sensors [54][55][56]…”

Section: Introductionmentioning

confidence: 99%

“…13, 16, 24, 41 and 47) and so on, relative humidity, 13,[48][49][50] temperature, 37 molecules in the liquid phase, 13,23,51,52 gas molecules, etc. Fluorescence sensors, 42,47,53,54 electrochemical sensors [54][55][56][57][58] and colorimetric sensors 43,46,54,59 are some of the widely explored CD-based sensor types. A fluorescence sensor uses variation in the fluorescence intensity/quenching of CDs on absorption and desorption of analytes, while colorimetric sensors use the shift in fluorescence peak of CDs as a sensing signal.…”

Section: Introductionmentioning

confidence: 99%

Recent development in carbon dot-based gas sensors

Swathilakshmi

Anandhan

2022

Sens. Diagn.

View full text Add to dashboard Cite

show abstract

“…The inhibition of AChE by various compounds was used in many analytical procedures like an assay of diazinon by AChE electrochemical biosensor [ 13 ], an assay of galantamine by a microcalorimetry AChE biosensor [ 14 ], a near-infrared light fluorometric biosensor for AChE activity assay [ 15 ], and screen-printed amperometric biosensor with immobilized AChE detecting phenylmethylsulfonylfluoride [ 16 ]. The authors of the cited papers chose performance for one inhibitor, but the devices are sensitive to multiple inhibitors.…”

Section: Introductionmentioning

confidence: 99%

A Butyrylcholinesterase Camera Biosensor Tested for Carbofuran and Paraoxon Assay

Pohanka

Zakova

2022

International Journal of Analytical Chemistry

View full text Add to dashboard Cite

Biosensors containing cholinesterase are analytical devices suitable for the assay of neurotoxic compounds. In the research on biosensors, a new platform has appeared some years ago. It is the digital photography and scoring of coloration (photogrammetry). In this paper, a colorimetric biosensor is constructed using 3D-printed multiwell pads treated with indoxylacetate as a chromogenic substrate and gold nanoparticles with the immobilized enzyme butyrylcholinesterase. A smartphone camera served for photogrammetry. The biosensor was tested for the assay of carbofuran and paraoxon ethyl as two types of covalently binding inhibitors: irreversible and pseudoirreversible. The biosensor exerted good sensitivity to the inhibitors and was able to detect carbofuran with a limit of detection for carbofuran 7.7 nmol/l and 17.6 nmol/l for paraoxon ethyl. A sample sized 25 μl was suitable for the assay lasting approximately 70 minutes. Up to 121 samples can be measured contemporary using one multiwell pad. The received data fully correlated with the standard spectrophotometry. The colorimetric biosensor exerts promising specifications and appears to be competitive to the other analytical procedures working on the principle of cholinesterase inhibition. Low-cost, simple, and portable design represent an advantage of the assay of the biosensor. Despite the overall simplicity, the biosensor can fully replace the standard spectroscopic methods.

show abstract

Assessment of pesticide induced inhibition of Apis mellifera (honeybee) acetylcholinesterase by means of N-doped carbon dots/BSA nanocomposite modified electrochemical biosensor

Cited by 26 publications

References 55 publications

2,3-Dihydroquinazolin-4(1H)-one as a New Class of Anti-Leishmanial Agents: A Combined Experimental and Computational Study

2,3-Dihydroquinazolin-4(1H)-one as a New Class of Anti-Leishmanial Agents: A Combined Experimental and Computational Study

Recent development in carbon dot-based gas sensors

A Butyrylcholinesterase Camera Biosensor Tested for Carbofuran and Paraoxon Assay

Contact Info

Product

Resources

About